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Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.

Song W, Yuan J, Zhang Z, Li L, Hu L - Exp Ther Med (2014)

Bottom Line: In patients with SLE, the levels of GCL activity and GSH decreased, while TRX and GSSG levels increased when compared with those in the healthy controls.GSH concentrations and GCL activity levels negatively correlated with the SLE disease activity index and erythrocyte sedimentation rate.Furthermore, patients with SLE and nephritis had lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis.

View Article: PubMed Central - PubMed

Affiliation: Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China ; Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China.

ABSTRACT
Reductions in glutathione (GSH) levels have been shown to be associated with aging and the pathogenesis of a variety of diseases, including systemic lupus erythematosus (SLE). Glutamate cysteine ligase (GCL) catalyzes the first and rate-limiting step of GSH synthesis. In order to appraise the correlation between oxidative stress and the severity and activity of SLE, GSH, oxidized GSH (GSSG) and thioredoxin (TRX) concentrations and the enzymatic activity levels of GCL in peripheral blood mononuclear cells (PBMCs) from patients with SLE and healthy controls were studied. In patients with SLE, the levels of GCL activity and GSH decreased, while TRX and GSSG levels increased when compared with those in the healthy controls. GSH concentrations and GCL activity levels negatively correlated with the SLE disease activity index and erythrocyte sedimentation rate. Furthermore, patients with SLE and nephritis had lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis. Therefore, the results of the present study indicate that insufficient levels of GSH and GCL activity in PBMCs may contribute to the pathogenesis of SLE.

No MeSH data available.


Related in: MedlinePlus

GSH levels in PBMCs from patients with SLE (n=30) and controls (n=30). Horizontal lines indicate mean values (SLE patients, 274.9; controls, 413.6 nmol/mg protein). There was a significant reduction in GSH levels in the SLE patients compared with the controls (P<0.0001). GSH, glutathione; PBMCs, peripheral blood mononuclear cells; SLE, systemic lupus erythematosus.
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f1-etm-08-01-0195: GSH levels in PBMCs from patients with SLE (n=30) and controls (n=30). Horizontal lines indicate mean values (SLE patients, 274.9; controls, 413.6 nmol/mg protein). There was a significant reduction in GSH levels in the SLE patients compared with the controls (P<0.0001). GSH, glutathione; PBMCs, peripheral blood mononuclear cells; SLE, systemic lupus erythematosus.

Mentions: In order to explore the role of oxidative stress in SLE, GSH and GSSG concentrations and the redox state (GSH/GSSG), were examined in PBMCs from 30 patients with SLE and 30 gender- and age-matched healthy controls. GSH levels considerably decreased in PBMCs from the patients with SLE (274.90±17.08 nmol/mg protein) compared with those in the healthy controls (413.63±20.79 nmol/mg protein; P<0.0001; Fig. 1). By contrast, GSSG levels significantly increased (124.95±4.27 nmol/mg protein; P<0.01) in patients with SLE compared with those in the healthy controls (68.94±1.89 nmol/mg protein; Table II). The reduction in GSH and increase in GSSG concentrations resulted in a decreased redox state (GSH/GSSG) in patients with SLE (2.27±0.43; P<0.001) compared with that in the healthy controls (6.11±1.07; Table II). Furthermore, the levels of the antioxidant GSH were markedly decreased in patients with SLE and LN (243.33±16.73 nmol/mg protein) compared with those in SLE patients without LN (322.25±30.58 nmol/mg protein; P<0.02; Fig. 2). The level of GSSG significantly increased (121.06±8.32 nmol/mg protein; P<0.05) and the redox state markedly decreased (2.01±0.58; P<0.05) in patients with SLE and LN compared with those in SLE patients without LN (Table II).


Altered glutamate cysteine ligase activity in peripheral blood mononuclear cells from patients with systemic lupus erythematosus.

Song W, Yuan J, Zhang Z, Li L, Hu L - Exp Ther Med (2014)

GSH levels in PBMCs from patients with SLE (n=30) and controls (n=30). Horizontal lines indicate mean values (SLE patients, 274.9; controls, 413.6 nmol/mg protein). There was a significant reduction in GSH levels in the SLE patients compared with the controls (P<0.0001). GSH, glutathione; PBMCs, peripheral blood mononuclear cells; SLE, systemic lupus erythematosus.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061188&req=5

f1-etm-08-01-0195: GSH levels in PBMCs from patients with SLE (n=30) and controls (n=30). Horizontal lines indicate mean values (SLE patients, 274.9; controls, 413.6 nmol/mg protein). There was a significant reduction in GSH levels in the SLE patients compared with the controls (P<0.0001). GSH, glutathione; PBMCs, peripheral blood mononuclear cells; SLE, systemic lupus erythematosus.
Mentions: In order to explore the role of oxidative stress in SLE, GSH and GSSG concentrations and the redox state (GSH/GSSG), were examined in PBMCs from 30 patients with SLE and 30 gender- and age-matched healthy controls. GSH levels considerably decreased in PBMCs from the patients with SLE (274.90±17.08 nmol/mg protein) compared with those in the healthy controls (413.63±20.79 nmol/mg protein; P<0.0001; Fig. 1). By contrast, GSSG levels significantly increased (124.95±4.27 nmol/mg protein; P<0.01) in patients with SLE compared with those in the healthy controls (68.94±1.89 nmol/mg protein; Table II). The reduction in GSH and increase in GSSG concentrations resulted in a decreased redox state (GSH/GSSG) in patients with SLE (2.27±0.43; P<0.001) compared with that in the healthy controls (6.11±1.07; Table II). Furthermore, the levels of the antioxidant GSH were markedly decreased in patients with SLE and LN (243.33±16.73 nmol/mg protein) compared with those in SLE patients without LN (322.25±30.58 nmol/mg protein; P<0.02; Fig. 2). The level of GSSG significantly increased (121.06±8.32 nmol/mg protein; P<0.05) and the redox state markedly decreased (2.01±0.58; P<0.05) in patients with SLE and LN compared with those in SLE patients without LN (Table II).

Bottom Line: In patients with SLE, the levels of GCL activity and GSH decreased, while TRX and GSSG levels increased when compared with those in the healthy controls.GSH concentrations and GCL activity levels negatively correlated with the SLE disease activity index and erythrocyte sedimentation rate.Furthermore, patients with SLE and nephritis had lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis.

View Article: PubMed Central - PubMed

Affiliation: Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430030, P.R. China ; Qingdao Municipal Hospital, Qingdao, Shandong 266071, P.R. China.

ABSTRACT
Reductions in glutathione (GSH) levels have been shown to be associated with aging and the pathogenesis of a variety of diseases, including systemic lupus erythematosus (SLE). Glutamate cysteine ligase (GCL) catalyzes the first and rate-limiting step of GSH synthesis. In order to appraise the correlation between oxidative stress and the severity and activity of SLE, GSH, oxidized GSH (GSSG) and thioredoxin (TRX) concentrations and the enzymatic activity levels of GCL in peripheral blood mononuclear cells (PBMCs) from patients with SLE and healthy controls were studied. In patients with SLE, the levels of GCL activity and GSH decreased, while TRX and GSSG levels increased when compared with those in the healthy controls. GSH concentrations and GCL activity levels negatively correlated with the SLE disease activity index and erythrocyte sedimentation rate. Furthermore, patients with SLE and nephritis had lower levels of GSH and GCL activity and higher levels of TRX and GSSG compared with those in SLE patients without nephritis. Therefore, the results of the present study indicate that insufficient levels of GSH and GCL activity in PBMCs may contribute to the pathogenesis of SLE.

No MeSH data available.


Related in: MedlinePlus