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Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms.

Lu Q, Luo S, Wen Y - Exp Ther Med (2014)

Bottom Line: The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined.Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted.In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical and Molecular Biology, School of Basic Courses, Guangdong Phamaceutical University, Guangzhou, Guangdong 510006, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of ligustilide (LIG) on angiotensin II (Ang II)-induced hypertrophy in neonatal rat myocardial cells and the expression levels of p53, Bcl-2 and Bax. Myocardial cells were isolated and purified from the ventricles of neonate Sprague-Dawley rats (age, 1-3 days) using a differential adhesion method. The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined. Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted. LIG was shown to significantly suppress the Ang II-induced hypertrophy of neonatal rat myocardial cells in a dose-dependent manner. In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax. Therefore, LIG can prevent the hypertrophy of cardiomyocytes induced by Ang II, which may be associated with the inhibitory effect that LIG exhibits on cardiomyocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus

Effects of LIG on the expression levels of Bcl-2 in cardiomyocytes (magnification, ×400; DAB & hematoxylin stained) in the (A) control at 1 day, (B) Ang II (1 μg/ml) at 1 day, (C) Ang II (1 μg/ml) + LIG (25 μg/ml) at 1 day, (D) Ang II (1 μg/ml) + LIG (50 μg/ml) at 1 day and (E) Ang II (1 μg/ml) + LIG (100 μg/ml) at 1 day. LIG, ligustilide; Ang II, angiotensin II.
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f6-etm-08-01-0169: Effects of LIG on the expression levels of Bcl-2 in cardiomyocytes (magnification, ×400; DAB & hematoxylin stained) in the (A) control at 1 day, (B) Ang II (1 μg/ml) at 1 day, (C) Ang II (1 μg/ml) + LIG (25 μg/ml) at 1 day, (D) Ang II (1 μg/ml) + LIG (50 μg/ml) at 1 day and (E) Ang II (1 μg/ml) + LIG (100 μg/ml) at 1 day. LIG, ligustilide; Ang II, angiotensin II.

Mentions: Expression levels of p53, Bcl-2 and Bax in the primary rat myocardial cells were detected by immunocytochemistry analysis. The expression of p53 and Bax in the myocardial cells was markedly induced with Ang II treatment, which was observed as black staining in the nuclei. However, the expression of these proteins was inhibited with various doses of LIG, with administration of 100 μg/ml LIG restoring the expression levels of p53 and Bax almost to the levels in the normal myocardial cells (Figs. 4 and 5). By contrast, the expression of Bcl-2 was significantly suppressed with Ang II treatment, although this was restored by various doses of LIG. With increasing doses of LIG, the expression levels of Bcl-2 gradually increased, as shown by the black staining (Fig. 6).


Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms.

Lu Q, Luo S, Wen Y - Exp Ther Med (2014)

Effects of LIG on the expression levels of Bcl-2 in cardiomyocytes (magnification, ×400; DAB & hematoxylin stained) in the (A) control at 1 day, (B) Ang II (1 μg/ml) at 1 day, (C) Ang II (1 μg/ml) + LIG (25 μg/ml) at 1 day, (D) Ang II (1 μg/ml) + LIG (50 μg/ml) at 1 day and (E) Ang II (1 μg/ml) + LIG (100 μg/ml) at 1 day. LIG, ligustilide; Ang II, angiotensin II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061187&req=5

f6-etm-08-01-0169: Effects of LIG on the expression levels of Bcl-2 in cardiomyocytes (magnification, ×400; DAB & hematoxylin stained) in the (A) control at 1 day, (B) Ang II (1 μg/ml) at 1 day, (C) Ang II (1 μg/ml) + LIG (25 μg/ml) at 1 day, (D) Ang II (1 μg/ml) + LIG (50 μg/ml) at 1 day and (E) Ang II (1 μg/ml) + LIG (100 μg/ml) at 1 day. LIG, ligustilide; Ang II, angiotensin II.
Mentions: Expression levels of p53, Bcl-2 and Bax in the primary rat myocardial cells were detected by immunocytochemistry analysis. The expression of p53 and Bax in the myocardial cells was markedly induced with Ang II treatment, which was observed as black staining in the nuclei. However, the expression of these proteins was inhibited with various doses of LIG, with administration of 100 μg/ml LIG restoring the expression levels of p53 and Bax almost to the levels in the normal myocardial cells (Figs. 4 and 5). By contrast, the expression of Bcl-2 was significantly suppressed with Ang II treatment, although this was restored by various doses of LIG. With increasing doses of LIG, the expression levels of Bcl-2 gradually increased, as shown by the black staining (Fig. 6).

Bottom Line: The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined.Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted.In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical and Molecular Biology, School of Basic Courses, Guangdong Phamaceutical University, Guangzhou, Guangdong 510006, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of ligustilide (LIG) on angiotensin II (Ang II)-induced hypertrophy in neonatal rat myocardial cells and the expression levels of p53, Bcl-2 and Bax. Myocardial cells were isolated and purified from the ventricles of neonate Sprague-Dawley rats (age, 1-3 days) using a differential adhesion method. The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined. Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted. LIG was shown to significantly suppress the Ang II-induced hypertrophy of neonatal rat myocardial cells in a dose-dependent manner. In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax. Therefore, LIG can prevent the hypertrophy of cardiomyocytes induced by Ang II, which may be associated with the inhibitory effect that LIG exhibits on cardiomyocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus