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Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms.

Lu Q, Luo S, Wen Y - Exp Ther Med (2014)

Bottom Line: The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined.Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted.In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical and Molecular Biology, School of Basic Courses, Guangdong Phamaceutical University, Guangzhou, Guangdong 510006, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of ligustilide (LIG) on angiotensin II (Ang II)-induced hypertrophy in neonatal rat myocardial cells and the expression levels of p53, Bcl-2 and Bax. Myocardial cells were isolated and purified from the ventricles of neonate Sprague-Dawley rats (age, 1-3 days) using a differential adhesion method. The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined. Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted. LIG was shown to significantly suppress the Ang II-induced hypertrophy of neonatal rat myocardial cells in a dose-dependent manner. In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax. Therefore, LIG can prevent the hypertrophy of cardiomyocytes induced by Ang II, which may be associated with the inhibitory effect that LIG exhibits on cardiomyocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus

Effects of LIG on the cardiomyocyte apoptotic rate. A, control; B, Ang II (1 μg/ml); C, Ang II (1 μg/ml) + LIG (25 μg/ml); D, Ang II (1 μg/ml) + LIG (50 μg/ml); E, Ang II (1 μg/ml) + LIG (100 μg/ml) *P<0.05, vs. control; #P<0.05, vs. Ang II group B. LIG, ligustilide; Ang II, angiotensin II.
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f3-etm-08-01-0169: Effects of LIG on the cardiomyocyte apoptotic rate. A, control; B, Ang II (1 μg/ml); C, Ang II (1 μg/ml) + LIG (25 μg/ml); D, Ang II (1 μg/ml) + LIG (50 μg/ml); E, Ang II (1 μg/ml) + LIG (100 μg/ml) *P<0.05, vs. control; #P<0.05, vs. Ang II group B. LIG, ligustilide; Ang II, angiotensin II.

Mentions: In order to determine whether LIG also affects the Ang II-induced apoptosis of myocardial cells, the apoptotic rates of cells treated with Ang II and/or various doses of LIG were determined. The apoptotic rate of myocardial cells induced by Ang II was markedly higher when compared with the control group (P<0.05). However, the apoptotic rate was significantly reduced with LIG administration (P<0.05; Fig. 3).


Effect of ligustilide on Ang II-induced hypertrophy in cardiomyocytes and the potential mechanisms.

Lu Q, Luo S, Wen Y - Exp Ther Med (2014)

Effects of LIG on the cardiomyocyte apoptotic rate. A, control; B, Ang II (1 μg/ml); C, Ang II (1 μg/ml) + LIG (25 μg/ml); D, Ang II (1 μg/ml) + LIG (50 μg/ml); E, Ang II (1 μg/ml) + LIG (100 μg/ml) *P<0.05, vs. control; #P<0.05, vs. Ang II group B. LIG, ligustilide; Ang II, angiotensin II.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061187&req=5

f3-etm-08-01-0169: Effects of LIG on the cardiomyocyte apoptotic rate. A, control; B, Ang II (1 μg/ml); C, Ang II (1 μg/ml) + LIG (25 μg/ml); D, Ang II (1 μg/ml) + LIG (50 μg/ml); E, Ang II (1 μg/ml) + LIG (100 μg/ml) *P<0.05, vs. control; #P<0.05, vs. Ang II group B. LIG, ligustilide; Ang II, angiotensin II.
Mentions: In order to determine whether LIG also affects the Ang II-induced apoptosis of myocardial cells, the apoptotic rates of cells treated with Ang II and/or various doses of LIG were determined. The apoptotic rate of myocardial cells induced by Ang II was markedly higher when compared with the control group (P<0.05). However, the apoptotic rate was significantly reduced with LIG administration (P<0.05; Fig. 3).

Bottom Line: The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined.Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted.In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax.

View Article: PubMed Central - PubMed

Affiliation: Department of Biochemical and Molecular Biology, School of Basic Courses, Guangdong Phamaceutical University, Guangzhou, Guangdong 510006, P.R. China.

ABSTRACT
The aim of the present study was to investigate the effect of ligustilide (LIG) on angiotensin II (Ang II)-induced hypertrophy in neonatal rat myocardial cells and the expression levels of p53, Bcl-2 and Bax. Myocardial cells were isolated and purified from the ventricles of neonate Sprague-Dawley rats (age, 1-3 days) using a differential adhesion method. The cells were then were stimulated by Ang II and LIG for 1-3 days, following which the cell surface area, intracellular protein concentration, rate of apoptosis and the expression levels of p53, Bcl-2 and Bax were determined. Following stimulation with Ang II, the cell surface area of the neonatal rat myocardial cells increased significantly and the cell morphology was distorted. LIG was shown to significantly suppress the Ang II-induced hypertrophy of neonatal rat myocardial cells in a dose-dependent manner. In addition, administration of LIG restored the expression levels of p53, Bcl-2 and Bax. Therefore, LIG can prevent the hypertrophy of cardiomyocytes induced by Ang II, which may be associated with the inhibitory effect that LIG exhibits on cardiomyocyte apoptosis.

No MeSH data available.


Related in: MedlinePlus