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Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus

mRNA expression of IL-6 in the liver tissue. BDL in the control group significantly upregulated the mRNA expression of proinflammatory cytokine IL-6 in the liver tissue. Histone preconditioning (HPC group) significantly ameliorated OJ liver injury induced by BDL, as indicated by the decreased mRNA expression levels of IL-6 in the liver tissue. *P<0.05, compared with the control group. IL-6, interleukin-6; BDL, bile duct ligation; OJ, obstructive jaundice.
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f4-etm-08-01-0015: mRNA expression of IL-6 in the liver tissue. BDL in the control group significantly upregulated the mRNA expression of proinflammatory cytokine IL-6 in the liver tissue. Histone preconditioning (HPC group) significantly ameliorated OJ liver injury induced by BDL, as indicated by the decreased mRNA expression levels of IL-6 in the liver tissue. *P<0.05, compared with the control group. IL-6, interleukin-6; BDL, bile duct ligation; OJ, obstructive jaundice.

Mentions: Using qPCR, it was demonstrated that the mRNA expression levels of IL-6 were significantly upregulated by BDL in the control group compared with the sham group (P<0.05; Fig. 4), which is consistent with previous studies (21). However, compared with the control group, histone preconditioning (HPC group) significantly downregulated the mRNA expression levels of IL-6 (P<0.05; Fig. 4). In addition, BDL in the control group significantly upregulated the mRNA expression levels of TLR-4 and TLR-9 (P<0.05; Fig. 5) compared with the sham group, which is consistent with previous studies (4). In the present study it was demonstrated that histone preconditioning significantly ameliorated the upregulation of the mRNA expression levels of TLR-4 and TLR-9. Animals preconditioned with histones expressed significantly lower mRNA levels of TLR-4 and TLR-9 (P<0.05; Fig. 5) compared with animals in the control group.


Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

mRNA expression of IL-6 in the liver tissue. BDL in the control group significantly upregulated the mRNA expression of proinflammatory cytokine IL-6 in the liver tissue. Histone preconditioning (HPC group) significantly ameliorated OJ liver injury induced by BDL, as indicated by the decreased mRNA expression levels of IL-6 in the liver tissue. *P<0.05, compared with the control group. IL-6, interleukin-6; BDL, bile duct ligation; OJ, obstructive jaundice.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061184&req=5

f4-etm-08-01-0015: mRNA expression of IL-6 in the liver tissue. BDL in the control group significantly upregulated the mRNA expression of proinflammatory cytokine IL-6 in the liver tissue. Histone preconditioning (HPC group) significantly ameliorated OJ liver injury induced by BDL, as indicated by the decreased mRNA expression levels of IL-6 in the liver tissue. *P<0.05, compared with the control group. IL-6, interleukin-6; BDL, bile duct ligation; OJ, obstructive jaundice.
Mentions: Using qPCR, it was demonstrated that the mRNA expression levels of IL-6 were significantly upregulated by BDL in the control group compared with the sham group (P<0.05; Fig. 4), which is consistent with previous studies (21). However, compared with the control group, histone preconditioning (HPC group) significantly downregulated the mRNA expression levels of IL-6 (P<0.05; Fig. 4). In addition, BDL in the control group significantly upregulated the mRNA expression levels of TLR-4 and TLR-9 (P<0.05; Fig. 5) compared with the sham group, which is consistent with previous studies (4). In the present study it was demonstrated that histone preconditioning significantly ameliorated the upregulation of the mRNA expression levels of TLR-4 and TLR-9. Animals preconditioned with histones expressed significantly lower mRNA levels of TLR-4 and TLR-9 (P<0.05; Fig. 5) compared with animals in the control group.

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus