Limits...
Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus

Alterations in serum TB and DB 14 days after BDL and the effect of histone preconditioning. Serum levels of TB and DB in the control group were significantly higher compared with the sham group 14 days subsequent to BDL. Histone preconditioning (HPC group) did not significantly alter the serum levels of TB and DB. *P<0.05, compared with the control using Student’s t-test. TB, total bilirubin; DB, direct bilirubin; BDL, bile duct ligation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4061184&req=5

f2-etm-08-01-0015: Alterations in serum TB and DB 14 days after BDL and the effect of histone preconditioning. Serum levels of TB and DB in the control group were significantly higher compared with the sham group 14 days subsequent to BDL. Histone preconditioning (HPC group) did not significantly alter the serum levels of TB and DB. *P<0.05, compared with the control using Student’s t-test. TB, total bilirubin; DB, direct bilirubin; BDL, bile duct ligation.

Mentions: BDL in the control group resulted in significantly elevated serum levels of TB and DB (Fig. 2) compared with the sham group, which suggests that the experimental OJ model was successfully induced. The serum levels of TB and DB in rats preconditioned with histone proteins prior to being subjected to BDL were not significantly different from those in the control animals (P>0.05), indicating that the degree of cholestasis was similar in the two experimental groups (Fig. 2). OJ liver injury induced by BDL was prominent in the control group, as shown by the significantly increased serum levels of ALT (Fig. 3) compared with the sham group (P<0.05). In accordance with the results of histopathological studies, histone preconditioning significantly ameliorated OJ-induced liver injury induced by BDL. The serum levels of ALT were significantly lower than that of the control group (P<0.05; Fig. 3).


Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

Alterations in serum TB and DB 14 days after BDL and the effect of histone preconditioning. Serum levels of TB and DB in the control group were significantly higher compared with the sham group 14 days subsequent to BDL. Histone preconditioning (HPC group) did not significantly alter the serum levels of TB and DB. *P<0.05, compared with the control using Student’s t-test. TB, total bilirubin; DB, direct bilirubin; BDL, bile duct ligation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061184&req=5

f2-etm-08-01-0015: Alterations in serum TB and DB 14 days after BDL and the effect of histone preconditioning. Serum levels of TB and DB in the control group were significantly higher compared with the sham group 14 days subsequent to BDL. Histone preconditioning (HPC group) did not significantly alter the serum levels of TB and DB. *P<0.05, compared with the control using Student’s t-test. TB, total bilirubin; DB, direct bilirubin; BDL, bile duct ligation.
Mentions: BDL in the control group resulted in significantly elevated serum levels of TB and DB (Fig. 2) compared with the sham group, which suggests that the experimental OJ model was successfully induced. The serum levels of TB and DB in rats preconditioned with histone proteins prior to being subjected to BDL were not significantly different from those in the control animals (P>0.05), indicating that the degree of cholestasis was similar in the two experimental groups (Fig. 2). OJ liver injury induced by BDL was prominent in the control group, as shown by the significantly increased serum levels of ALT (Fig. 3) compared with the sham group (P<0.05). In accordance with the results of histopathological studies, histone preconditioning significantly ameliorated OJ-induced liver injury induced by BDL. The serum levels of ALT were significantly lower than that of the control group (P<0.05; Fig. 3).

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus