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Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus

Effects of histone preconditioning on obstructive jaundice-induced liver injury indicated by hematoxylin and eosin staining. (A) In sham rats no histological alterations were observed (sham group). (B) Bile duct ligation in the control group resulted in severe liver injury, demonstrated by an increase in neutrophil infiltration into the liver, as well as an increase in (C) ductal proliferation and (D) severe necroinflammation. Histone preconditioning significantly ameliorated liver injury, shown by the (E) reduction in neutrophil infiltration into the liver and (F) and a decrease in the severity of necroinflammation.
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f1-etm-08-01-0015: Effects of histone preconditioning on obstructive jaundice-induced liver injury indicated by hematoxylin and eosin staining. (A) In sham rats no histological alterations were observed (sham group). (B) Bile duct ligation in the control group resulted in severe liver injury, demonstrated by an increase in neutrophil infiltration into the liver, as well as an increase in (C) ductal proliferation and (D) severe necroinflammation. Histone preconditioning significantly ameliorated liver injury, shown by the (E) reduction in neutrophil infiltration into the liver and (F) and a decrease in the severity of necroinflammation.

Mentions: No histological alterations were observed in animals in the sham group. Following euthanasia on day 14 after BDL, severe liver injury was observed in the control group, as indicated by an increase in neutrophil infiltration into the liver tissue, as well as an increase in ductal proliferation and significantly higher modified HAI scores (P<0.05) compared with the sham group. (Fig. 1; Table I).


Histone preconditioning protects against obstructive jaundice-induced liver injury in rats.

Zhou YX, Ni Y, Liu YB, Liu X - Exp Ther Med (2014)

Effects of histone preconditioning on obstructive jaundice-induced liver injury indicated by hematoxylin and eosin staining. (A) In sham rats no histological alterations were observed (sham group). (B) Bile duct ligation in the control group resulted in severe liver injury, demonstrated by an increase in neutrophil infiltration into the liver, as well as an increase in (C) ductal proliferation and (D) severe necroinflammation. Histone preconditioning significantly ameliorated liver injury, shown by the (E) reduction in neutrophil infiltration into the liver and (F) and a decrease in the severity of necroinflammation.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061184&req=5

f1-etm-08-01-0015: Effects of histone preconditioning on obstructive jaundice-induced liver injury indicated by hematoxylin and eosin staining. (A) In sham rats no histological alterations were observed (sham group). (B) Bile duct ligation in the control group resulted in severe liver injury, demonstrated by an increase in neutrophil infiltration into the liver, as well as an increase in (C) ductal proliferation and (D) severe necroinflammation. Histone preconditioning significantly ameliorated liver injury, shown by the (E) reduction in neutrophil infiltration into the liver and (F) and a decrease in the severity of necroinflammation.
Mentions: No histological alterations were observed in animals in the sham group. Following euthanasia on day 14 after BDL, severe liver injury was observed in the control group, as indicated by an increase in neutrophil infiltration into the liver tissue, as well as an increase in ductal proliferation and significantly higher modified HAI scores (P<0.05) compared with the sham group. (Fig. 1; Table I).

Bottom Line: Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules.The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed.However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations.

View Article: PubMed Central - PubMed

Affiliation: Department of Hepatobiliary Surgery, The First Affiliated Hospital of Shenzhen University, Shenzhen, Guangdong 518035, P.R. China.

ABSTRACT
A major consequence of obstructive jaundice (OJ) in clinical practice is the development of severe liver injury, and at present, no effective treatments have been developed to protect against it. Preconditioning with damage-associated molecular pattern (DAMP) molecules has been demonstrated to protect multiple organs from injury, and histones have been recently identified as DAMP molecules. The aim of the present study was to investigate the protective effect of histone preconditioning against OJ-induced liver injury in rats and the involvement of Toll-like receptors. Rats were administered histone proteins (200 μg/kg; 1 ml) or physiological saline (1 ml) intraperitoneally 24 h prior to being subjected to bile duct ligation (BDL). The serum levels of liver enzymes and bilirubin, as well as the histopathology were analyzed. The mRNA expression of interleukin-6 (IL-6) in the liver tissue was analyzed using quantitative polymerase chain reaction. BDL in the control group caused severe OJ-induced liver injury, as indicated by the significantly elevated levels of liver enzymes and mRNA levels of IL-6, and confirmed by histopathological alterations. However, histone preconditioning significantly ameliorated the OJ-induced liver injury caused by BDL, as shown by an improvement in the levels of liver enzymes, a suppression of IL-6 production, as well as histopathological alterations. Therefore, these results suggested that histone preconditioning is able to protect against OJ-induced liver injury in rats.

No MeSH data available.


Related in: MedlinePlus