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Indole-3-carbinol inhibits nasopharyngeal carcinoma cell growth in vivo and in vitro through inhibition of the PI3K/Akt pathway.

Mao CG, Tao ZZ, Chen Z, Chen C, Chen SM, Wan LJ - Exp Ther Med (2014)

Bottom Line: The results demonstrated that with increasing I3C concentrations, cell proliferation decreased and apoptosis increased significantly.In addition, the expression levels of PI3K/Akt pathway-associated proteins decreased.Therefore, the results of the present study indicated that I3C inhibited the growth of NPC cells and induced apoptosis effectively in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Indole-3-carbinol (I3C) is an active component of cruciferous vegetables and has been shown to markedly inhibit the growth of a variety of tumors. However, the role of I3C in nasopharyngeal carcinoma (NPC) remains unclear. Thus, the aim of the present study was to investigate the inhibition of NPC cells by I3C in vitro and in vivo. The human CNE2 NPC cell line was treated with various concentrations (0, 100, 200 and 300 μM) of I3C and analysis of cell proliferation after 0, 24, 48 and 72 h, apoptosis after 48 h and expression levels of phosphatidylinositol 3-kinase (PI3K)/Akt pathway-associated proteins in vitro was performed. BALB/c nude mice were divided into the following groups: Prevention, treatment and control. In vivo, all the nude mice were inoculated with CNE2 NPC cells and the mice in the prevention and treatment groups were administered a diet containing 0.5% I3C prior to and following inoculation, respectively. The tumoricidal effect of I3C was investigated in the nude mice. After eight weeks, the expression levels of PI3K/Akt pathway-associated proteins were analyzed in the tumors from the nude mice in each group. The results demonstrated that with increasing I3C concentrations, cell proliferation decreased and apoptosis increased significantly. In addition, the expression levels of PI3K/Akt pathway-associated proteins decreased. In the animal experiments, the prevention and treatment groups developed smaller tumors and the expression levels of PI3K/Akt pathway-associated proteins were reduced when compared with those in the control group. In addition, very few changes to the heart, liver and kidney tissues were observed with hematoxylin and eosin staining in all the groups. Therefore, the results of the present study indicated that I3C inhibited the growth of NPC cells and induced apoptosis effectively in vivo and in vitro. The underlying mechanism may be that I3C suppresses the PI3K/Akt pathway.

No MeSH data available.


Related in: MedlinePlus

I3C inhibition of tumor growth via the PI3K/Akt pathway in a CNE2 xenograft tumor model. Eight weeks following tumor implantation, the expression levels of PI3K/Akt pathway-associated proteins were analyzed by western blot analysis in the tumor tissues of the protective, treatment and control groups. GAPDH served as an internal reference control. PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; I3C, indole-3-carbinol.
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f4-etm-08-01-0207: I3C inhibition of tumor growth via the PI3K/Akt pathway in a CNE2 xenograft tumor model. Eight weeks following tumor implantation, the expression levels of PI3K/Akt pathway-associated proteins were analyzed by western blot analysis in the tumor tissues of the protective, treatment and control groups. GAPDH served as an internal reference control. PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; I3C, indole-3-carbinol.

Mentions: Expression levels of proteins associated with the PI3K/Akt pathway and downstream signaling pathways were analyzed in the transplanted tumors. Pretreatment and treatment with I3C was found to be associated with reductions in the expression levels of PI3K p110α, PI3K p85, p-Akt and p-GSK3-β signaling proteins. The lowest expression levels of signaling proteins were detected in the pretreatment group, which was followed by the treatment group. The highest expression levels of signaling proteins were identified in the control group (Fig. 4).


Indole-3-carbinol inhibits nasopharyngeal carcinoma cell growth in vivo and in vitro through inhibition of the PI3K/Akt pathway.

Mao CG, Tao ZZ, Chen Z, Chen C, Chen SM, Wan LJ - Exp Ther Med (2014)

I3C inhibition of tumor growth via the PI3K/Akt pathway in a CNE2 xenograft tumor model. Eight weeks following tumor implantation, the expression levels of PI3K/Akt pathway-associated proteins were analyzed by western blot analysis in the tumor tissues of the protective, treatment and control groups. GAPDH served as an internal reference control. PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; I3C, indole-3-carbinol.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061183&req=5

f4-etm-08-01-0207: I3C inhibition of tumor growth via the PI3K/Akt pathway in a CNE2 xenograft tumor model. Eight weeks following tumor implantation, the expression levels of PI3K/Akt pathway-associated proteins were analyzed by western blot analysis in the tumor tissues of the protective, treatment and control groups. GAPDH served as an internal reference control. PI3K, phosphatidylinositol 3-kinase; Akt, protein kinase B; I3C, indole-3-carbinol.
Mentions: Expression levels of proteins associated with the PI3K/Akt pathway and downstream signaling pathways were analyzed in the transplanted tumors. Pretreatment and treatment with I3C was found to be associated with reductions in the expression levels of PI3K p110α, PI3K p85, p-Akt and p-GSK3-β signaling proteins. The lowest expression levels of signaling proteins were detected in the pretreatment group, which was followed by the treatment group. The highest expression levels of signaling proteins were identified in the control group (Fig. 4).

Bottom Line: The results demonstrated that with increasing I3C concentrations, cell proliferation decreased and apoptosis increased significantly.In addition, the expression levels of PI3K/Akt pathway-associated proteins decreased.Therefore, the results of the present study indicated that I3C inhibited the growth of NPC cells and induced apoptosis effectively in vivo and in vitro.

View Article: PubMed Central - PubMed

Affiliation: Department of Otolaryngology-Head and Neck Surgery, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, P.R. China.

ABSTRACT
Indole-3-carbinol (I3C) is an active component of cruciferous vegetables and has been shown to markedly inhibit the growth of a variety of tumors. However, the role of I3C in nasopharyngeal carcinoma (NPC) remains unclear. Thus, the aim of the present study was to investigate the inhibition of NPC cells by I3C in vitro and in vivo. The human CNE2 NPC cell line was treated with various concentrations (0, 100, 200 and 300 μM) of I3C and analysis of cell proliferation after 0, 24, 48 and 72 h, apoptosis after 48 h and expression levels of phosphatidylinositol 3-kinase (PI3K)/Akt pathway-associated proteins in vitro was performed. BALB/c nude mice were divided into the following groups: Prevention, treatment and control. In vivo, all the nude mice were inoculated with CNE2 NPC cells and the mice in the prevention and treatment groups were administered a diet containing 0.5% I3C prior to and following inoculation, respectively. The tumoricidal effect of I3C was investigated in the nude mice. After eight weeks, the expression levels of PI3K/Akt pathway-associated proteins were analyzed in the tumors from the nude mice in each group. The results demonstrated that with increasing I3C concentrations, cell proliferation decreased and apoptosis increased significantly. In addition, the expression levels of PI3K/Akt pathway-associated proteins decreased. In the animal experiments, the prevention and treatment groups developed smaller tumors and the expression levels of PI3K/Akt pathway-associated proteins were reduced when compared with those in the control group. In addition, very few changes to the heart, liver and kidney tissues were observed with hematoxylin and eosin staining in all the groups. Therefore, the results of the present study indicated that I3C inhibited the growth of NPC cells and induced apoptosis effectively in vivo and in vitro. The underlying mechanism may be that I3C suppresses the PI3K/Akt pathway.

No MeSH data available.


Related in: MedlinePlus