Limits...
Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.

Feng Z, Hao W, Lin X, Fan D, Zhou J - Onco Targets Ther (2014)

Bottom Line: The effects of total flavonoids on tumor growth in vivo were examined.Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China ; Department of Oncology, Guang An Men Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

ABSTRACT

Objective: To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice.

Materials and methods: Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.

Results: Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs.

Conclusion: The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

No MeSH data available.


Related in: MedlinePlus

(A) Effects of flavonoids from Radix Tetrastigmae on tumor weight.Notes: The weights of tumors in mice were measured at day 14 after Lewis lung carcinoma tumor inoculation and flavonoid administration. (B) Inhibitory activities of flavonoids in tumor weight increase are shown. (C) Similar to increases in tumor weight, the inhibitory activities of flavonoids in tumor volume increase were also presented. The cellular structures of tumor cells from mice at day 14 after 5 mg/kg flavonoid administration were examined by electron microscopy (E), and compared with those from mice without flavonoid administration (D). Arrow bar in (E) indicates the bubble-like structures in tumor cells induced by flavonoid treatment. All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4061169&req=5

f3-ott-7-947: (A) Effects of flavonoids from Radix Tetrastigmae on tumor weight.Notes: The weights of tumors in mice were measured at day 14 after Lewis lung carcinoma tumor inoculation and flavonoid administration. (B) Inhibitory activities of flavonoids in tumor weight increase are shown. (C) Similar to increases in tumor weight, the inhibitory activities of flavonoids in tumor volume increase were also presented. The cellular structures of tumor cells from mice at day 14 after 5 mg/kg flavonoid administration were examined by electron microscopy (E), and compared with those from mice without flavonoid administration (D). Arrow bar in (E) indicates the bubble-like structures in tumor cells induced by flavonoid treatment. All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.

Mentions: At day 14, the administration of total flavonoids from Radix Tetrastigmae at a high dose significantly suppressed the increase of tumor weights in mice (Figure 3A and B). Interestingly, the administration of total flavonoids from Radix Tetrastigmae at a low dose at day 14 also significantly inhibited the increase of tumor volumes in mice (Figure 3C). As compared with tumor cells from mice without flavonoid administration (Figure 3D), analysis by transmission electron microscopy indicated that the treatment by a low dose of total flavonoids induced the formation of irregular nucleus and bubble-like structures in the cytoplasm and loss of normal nucleoli (Figure 3E), suggesting that flavonoid administration led to apoptosis of tumor cells in mice. Thus, it appeared that flavonoids from Radix Tetrastigmae suppressed tumor growth in vivo.


Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.

Feng Z, Hao W, Lin X, Fan D, Zhou J - Onco Targets Ther (2014)

(A) Effects of flavonoids from Radix Tetrastigmae on tumor weight.Notes: The weights of tumors in mice were measured at day 14 after Lewis lung carcinoma tumor inoculation and flavonoid administration. (B) Inhibitory activities of flavonoids in tumor weight increase are shown. (C) Similar to increases in tumor weight, the inhibitory activities of flavonoids in tumor volume increase were also presented. The cellular structures of tumor cells from mice at day 14 after 5 mg/kg flavonoid administration were examined by electron microscopy (E), and compared with those from mice without flavonoid administration (D). Arrow bar in (E) indicates the bubble-like structures in tumor cells induced by flavonoid treatment. All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061169&req=5

f3-ott-7-947: (A) Effects of flavonoids from Radix Tetrastigmae on tumor weight.Notes: The weights of tumors in mice were measured at day 14 after Lewis lung carcinoma tumor inoculation and flavonoid administration. (B) Inhibitory activities of flavonoids in tumor weight increase are shown. (C) Similar to increases in tumor weight, the inhibitory activities of flavonoids in tumor volume increase were also presented. The cellular structures of tumor cells from mice at day 14 after 5 mg/kg flavonoid administration were examined by electron microscopy (E), and compared with those from mice without flavonoid administration (D). Arrow bar in (E) indicates the bubble-like structures in tumor cells induced by flavonoid treatment. All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
Mentions: At day 14, the administration of total flavonoids from Radix Tetrastigmae at a high dose significantly suppressed the increase of tumor weights in mice (Figure 3A and B). Interestingly, the administration of total flavonoids from Radix Tetrastigmae at a low dose at day 14 also significantly inhibited the increase of tumor volumes in mice (Figure 3C). As compared with tumor cells from mice without flavonoid administration (Figure 3D), analysis by transmission electron microscopy indicated that the treatment by a low dose of total flavonoids induced the formation of irregular nucleus and bubble-like structures in the cytoplasm and loss of normal nucleoli (Figure 3E), suggesting that flavonoid administration led to apoptosis of tumor cells in mice. Thus, it appeared that flavonoids from Radix Tetrastigmae suppressed tumor growth in vivo.

Bottom Line: The effects of total flavonoids on tumor growth in vivo were examined.Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China ; Department of Oncology, Guang An Men Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

ABSTRACT

Objective: To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice.

Materials and methods: Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.

Results: Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs.

Conclusion: The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

No MeSH data available.


Related in: MedlinePlus