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Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.

Feng Z, Hao W, Lin X, Fan D, Zhou J - Onco Targets Ther (2014)

Bottom Line: The effects of total flavonoids on tumor growth in vivo were examined.Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China ; Department of Oncology, Guang An Men Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

ABSTRACT

Objective: To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice.

Materials and methods: Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.

Results: Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs.

Conclusion: The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

No MeSH data available.


Related in: MedlinePlus

Effects of flavonoids from Radix Tetrastigmae on mouse body weight.Notes: The body weights of mice were determined before Lewis lung carcinoma tumor inoculation and flavonoid administration (A) and at day 14 after tumor inoculation and treatment (B). All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
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f1-ott-7-947: Effects of flavonoids from Radix Tetrastigmae on mouse body weight.Notes: The body weights of mice were determined before Lewis lung carcinoma tumor inoculation and flavonoid administration (A) and at day 14 after tumor inoculation and treatment (B). All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.

Mentions: All groups of mice had similar baseline body weights (Figure 1A). At the end point, the group of mice receiving the LLC cell inoculation but without flavonoid treatment had significantly decreased body weights compared with the control group (Figure 1B), suggesting tumor growth suppressed the increase of mouse body weights. Administration of total flavonoids from Radix Tetrastigmae at higher doses significantly rescued the body weight gain in mice inoculated with LLC cells (Figure 1B). The results indicated that total flavonoids from Radix Tetrastigmae improved the overall health of the mice by suppressing tumor growth in mice.


Antitumor activity of total flavonoids from Tetrastigma hemsleyanum Diels et Gilg is associated with the inhibition of regulatory T cells in mice.

Feng Z, Hao W, Lin X, Fan D, Zhou J - Onco Targets Ther (2014)

Effects of flavonoids from Radix Tetrastigmae on mouse body weight.Notes: The body weights of mice were determined before Lewis lung carcinoma tumor inoculation and flavonoid administration (A) and at day 14 after tumor inoculation and treatment (B). All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061169&req=5

f1-ott-7-947: Effects of flavonoids from Radix Tetrastigmae on mouse body weight.Notes: The body weights of mice were determined before Lewis lung carcinoma tumor inoculation and flavonoid administration (A) and at day 14 after tumor inoculation and treatment (B). All P-values were calculated from comparison between the tumor inoculation group without flavonoid administration and other groups.
Mentions: All groups of mice had similar baseline body weights (Figure 1A). At the end point, the group of mice receiving the LLC cell inoculation but without flavonoid treatment had significantly decreased body weights compared with the control group (Figure 1B), suggesting tumor growth suppressed the increase of mouse body weights. Administration of total flavonoids from Radix Tetrastigmae at higher doses significantly rescued the body weight gain in mice inoculated with LLC cells (Figure 1B). The results indicated that total flavonoids from Radix Tetrastigmae improved the overall health of the mice by suppressing tumor growth in mice.

Bottom Line: The effects of total flavonoids on tumor growth in vivo were examined.Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Tongde Hospital of Zhejiang Province, Hangzhou, Zhejiang, People's Republic of China ; Department of Oncology, Guang An Men Hospital, China Academy of Chinese Medical Sciences, Beijing, People's Republic of China.

ABSTRACT

Objective: To determine the antitumor activity of Radix tetrastigmae flavonoids and their inhibitory effect on regulatory T cells (Tregs) in mice.

Materials and methods: Total flavonoids were isolated from Radix tetrastigmae, the root of Tetrastigma hemsleyanum Diels et Gilg, and administered to C57BL/6 mice by oral gavage after inoculation with Lewis lung carcinoma (LLC) cells. The effects of total flavonoids on tumor growth in vivo were examined. Flow cytometry was used to study the effects on Tregs, and enzyme-linked immunosorbent assay was used to analyze the changes in the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 after tumor inoculation and flavonoid administration.

Results: Total flavonoids from T. hemsleyanum Diels et Gilg significantly inhibited tumor growth in C57BL/6 mice inoculated with LLCs. These flavonoids dramatically suppressed regulatory T-cell development in tumor-bearing mice. Further studies revealed that total flavonoids significantly decreased the serum levels of transforming growth factor β, prostaglandin E2, and cyclooxygenase 2 in tumor-bearing mice, which may be responsible for the inhibition of Tregs.

Conclusion: The antitumor activity of total flavonoids from T. hemsleyanum Diels et Gilg is associated with the inhibition of Tregs in a mouse tumor model. Total flavonoids from T. hemsleyanum Diels et Gilg may be used as antitumor agents in cancer prevention and treatment.

No MeSH data available.


Related in: MedlinePlus