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Oral administration of heat-inactivated Lactobacillus plantarum K37 modulated airway hyperresponsiveness in ovalbumin-sensitized BALB/c mice.

Liu YW, Liao TW, Chen YH, Chiang YC, Tsai YC - PLoS ONE (2014)

Bottom Line: The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF).The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13.K37 also improved cell infiltration in lung sections.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.

ABSTRACT
This study aimed to investigate the anti-allergic effects of Lactobacillus plantarum K37 (K37) on airway hyperresponsiveness (AHR) and systemic allergic responses in ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Heat-inactivated K37 (105, 107, and 109 CFU/mouse, day) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on AHR, immunoglobulin (Ig) and cytokine production. The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF). The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13. K37 also improved cell infiltration in lung sections. Our results demonstrated that oral administration of K37 alleviated effectively the allergic responses in vivo. Thus, K37 can be a good source material and a promising candidate for prophylactic and therapeutic treatments of allergic diseases, like asthma.

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Related in: MedlinePlus

Effect of oral administration of K37 on cytokines production in BALF of OVA-sensitized mice.The concentration of (A) IL-2, (B) IL-12, (C) IFN-γ, (D) IL-4, (E) IL-5, (F) IL-13, (G) TNF-α, (H) IL-6, and (I) eotaxin in the BALF were determined by ELISA. Each value represents the mean ± SD, n = 8. A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
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pone-0100105-g006: Effect of oral administration of K37 on cytokines production in BALF of OVA-sensitized mice.The concentration of (A) IL-2, (B) IL-12, (C) IFN-γ, (D) IL-4, (E) IL-5, (F) IL-13, (G) TNF-α, (H) IL-6, and (I) eotaxin in the BALF were determined by ELISA. Each value represents the mean ± SD, n = 8. A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).

Mentions: The cytokine production profile was employed to evaluate the effects of K37 on T-cell responses. The concentrations of Th1 cytokines, IL-2, IL-12, and IFN-γ, and Th2 cytokines, IL-4, IL-5, and IL-13, in BALF (Figure 6) and in spleen cell cultures (Figure 7) were measured using the ELISA method. As shown in Figure 6A–C, levels of Th1 cytokines, IL-2, IL-12, and IFN-γ, in BALF were elevated dose-dependently in K37 groups compared with the OVA group. In the K37-H group, the levels of IL-2 and IL-12 were significantly higher than those in the OVA group (Figure 6A and 6C, P<0.05). The IFN-γ levels were significantly increased in both K37-M (P<0.05) and K37-H groups (P<0.01). Levels of Th2 cytokines in BALF, including IL-4, IL-5, and IL-13, were also measured. The levels of IL-4 in BALF of K37 groups (Figure 6D) were significantly decreased compared with that in the OVA group (K37-L, P<0.05; K37-M and K37-H, P<0.01). In both K37-M and K37-H groups, the levels of IL-5 were significantly lower than that in the OVA group (Figure 6C; K37-M, P<0.05 and K37-H, P<0.01). However, only in the K37-H group was the level of IL-13 significantly diminished (Figure 6F).


Oral administration of heat-inactivated Lactobacillus plantarum K37 modulated airway hyperresponsiveness in ovalbumin-sensitized BALB/c mice.

Liu YW, Liao TW, Chen YH, Chiang YC, Tsai YC - PLoS ONE (2014)

Effect of oral administration of K37 on cytokines production in BALF of OVA-sensitized mice.The concentration of (A) IL-2, (B) IL-12, (C) IFN-γ, (D) IL-4, (E) IL-5, (F) IL-13, (G) TNF-α, (H) IL-6, and (I) eotaxin in the BALF were determined by ELISA. Each value represents the mean ± SD, n = 8. A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061068&req=5

pone-0100105-g006: Effect of oral administration of K37 on cytokines production in BALF of OVA-sensitized mice.The concentration of (A) IL-2, (B) IL-12, (C) IFN-γ, (D) IL-4, (E) IL-5, (F) IL-13, (G) TNF-α, (H) IL-6, and (I) eotaxin in the BALF were determined by ELISA. Each value represents the mean ± SD, n = 8. A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
Mentions: The cytokine production profile was employed to evaluate the effects of K37 on T-cell responses. The concentrations of Th1 cytokines, IL-2, IL-12, and IFN-γ, and Th2 cytokines, IL-4, IL-5, and IL-13, in BALF (Figure 6) and in spleen cell cultures (Figure 7) were measured using the ELISA method. As shown in Figure 6A–C, levels of Th1 cytokines, IL-2, IL-12, and IFN-γ, in BALF were elevated dose-dependently in K37 groups compared with the OVA group. In the K37-H group, the levels of IL-2 and IL-12 were significantly higher than those in the OVA group (Figure 6A and 6C, P<0.05). The IFN-γ levels were significantly increased in both K37-M (P<0.05) and K37-H groups (P<0.01). Levels of Th2 cytokines in BALF, including IL-4, IL-5, and IL-13, were also measured. The levels of IL-4 in BALF of K37 groups (Figure 6D) were significantly decreased compared with that in the OVA group (K37-L, P<0.05; K37-M and K37-H, P<0.01). In both K37-M and K37-H groups, the levels of IL-5 were significantly lower than that in the OVA group (Figure 6C; K37-M, P<0.05 and K37-H, P<0.01). However, only in the K37-H group was the level of IL-13 significantly diminished (Figure 6F).

Bottom Line: The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF).The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13.K37 also improved cell infiltration in lung sections.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.

ABSTRACT
This study aimed to investigate the anti-allergic effects of Lactobacillus plantarum K37 (K37) on airway hyperresponsiveness (AHR) and systemic allergic responses in ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Heat-inactivated K37 (105, 107, and 109 CFU/mouse, day) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on AHR, immunoglobulin (Ig) and cytokine production. The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF). The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13. K37 also improved cell infiltration in lung sections. Our results demonstrated that oral administration of K37 alleviated effectively the allergic responses in vivo. Thus, K37 can be a good source material and a promising candidate for prophylactic and therapeutic treatments of allergic diseases, like asthma.

Show MeSH
Related in: MedlinePlus