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Oral administration of heat-inactivated Lactobacillus plantarum K37 modulated airway hyperresponsiveness in ovalbumin-sensitized BALB/c mice.

Liu YW, Liao TW, Chen YH, Chiang YC, Tsai YC - PLoS ONE (2014)

Bottom Line: The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF).The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13.K37 also improved cell infiltration in lung sections.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.

ABSTRACT
This study aimed to investigate the anti-allergic effects of Lactobacillus plantarum K37 (K37) on airway hyperresponsiveness (AHR) and systemic allergic responses in ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Heat-inactivated K37 (105, 107, and 109 CFU/mouse, day) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on AHR, immunoglobulin (Ig) and cytokine production. The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF). The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13. K37 also improved cell infiltration in lung sections. Our results demonstrated that oral administration of K37 alleviated effectively the allergic responses in vivo. Thus, K37 can be a good source material and a promising candidate for prophylactic and therapeutic treatments of allergic diseases, like asthma.

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Related in: MedlinePlus

Effect of oral administration of K37 on lung tissue inflammatory cell infiltration in OVA-sensitized mice after OVA challenge.(A) Total counts of cells in BALF from healthy control (CON), allergy control (OVA), and K37 (K37-L, K37-M, and K37-H) groups of mice. (B) Cell population of macrophage, eosinophil, neutrophil and lymphocyte in BALF were analysed and are expressed as the mean ± SD, (n = 8). A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
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pone-0100105-g004: Effect of oral administration of K37 on lung tissue inflammatory cell infiltration in OVA-sensitized mice after OVA challenge.(A) Total counts of cells in BALF from healthy control (CON), allergy control (OVA), and K37 (K37-L, K37-M, and K37-H) groups of mice. (B) Cell population of macrophage, eosinophil, neutrophil and lymphocyte in BALF were analysed and are expressed as the mean ± SD, (n = 8). A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).

Mentions: The numbers of macrophages, eosinophils, neutrophils and lymphocytes were counted to obtain the cell population of BALF for evaluating the effects of K37 on lung inflamamtion. The influx of inflammatory cells into lungs was examined. As shown in Figure 4A, the number of cells in both K37-M and K37-H groups were significantly lower when compared with that in the OVA group (P<0.05). The cell population of BALF was further analyzed (Figure 4B). In the OVA group, the percentage of eosinophils and neutrophils were significantly increased while the percentage of macrohages was decreased compared with the CON group. As for the K37-H group, the population of macrophage is elevated (P<0.01) while eosinophil infiltration is lowered (P<0.05) when compared with the OVA group.


Oral administration of heat-inactivated Lactobacillus plantarum K37 modulated airway hyperresponsiveness in ovalbumin-sensitized BALB/c mice.

Liu YW, Liao TW, Chen YH, Chiang YC, Tsai YC - PLoS ONE (2014)

Effect of oral administration of K37 on lung tissue inflammatory cell infiltration in OVA-sensitized mice after OVA challenge.(A) Total counts of cells in BALF from healthy control (CON), allergy control (OVA), and K37 (K37-L, K37-M, and K37-H) groups of mice. (B) Cell population of macrophage, eosinophil, neutrophil and lymphocyte in BALF were analysed and are expressed as the mean ± SD, (n = 8). A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061068&req=5

pone-0100105-g004: Effect of oral administration of K37 on lung tissue inflammatory cell infiltration in OVA-sensitized mice after OVA challenge.(A) Total counts of cells in BALF from healthy control (CON), allergy control (OVA), and K37 (K37-L, K37-M, and K37-H) groups of mice. (B) Cell population of macrophage, eosinophil, neutrophil and lymphocyte in BALF were analysed and are expressed as the mean ± SD, (n = 8). A difference between K37 groups and OVA group was considered statistically significant when P<0.05 (*) and P<0.01 (**).
Mentions: The numbers of macrophages, eosinophils, neutrophils and lymphocytes were counted to obtain the cell population of BALF for evaluating the effects of K37 on lung inflamamtion. The influx of inflammatory cells into lungs was examined. As shown in Figure 4A, the number of cells in both K37-M and K37-H groups were significantly lower when compared with that in the OVA group (P<0.05). The cell population of BALF was further analyzed (Figure 4B). In the OVA group, the percentage of eosinophils and neutrophils were significantly increased while the percentage of macrohages was decreased compared with the CON group. As for the K37-H group, the population of macrophage is elevated (P<0.01) while eosinophil infiltration is lowered (P<0.05) when compared with the OVA group.

Bottom Line: The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF).The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13.K37 also improved cell infiltration in lung sections.

View Article: PubMed Central - PubMed

Affiliation: Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Taipei, Taiwan, ROC.

ABSTRACT
This study aimed to investigate the anti-allergic effects of Lactobacillus plantarum K37 (K37) on airway hyperresponsiveness (AHR) and systemic allergic responses in ovalbumin (OVA)-sensitized and -challenged BALB/c mice. Heat-inactivated K37 (105, 107, and 109 CFU/mouse, day) were orally administered to OVA-sensitized BALB/c mice to investigate their effects on AHR, immunoglobulin (Ig) and cytokine production. The results showed that K37 dose-dependently lowered the serum levels of IgE, OVA-specific IgE and OVA-specific IgG1, ameliorated AHR induced by methacholine and suppressed eosinophil infiltration in bronchoalveolar lavage fluid (BALF). The cytokine production in spleen cells culture and BALF showed that K37 drove the immune responses toward T-helper cell type 1 (Th1) responses, elevated levels of IL-2 and IFN-γ, and reduced of IL-4, IL-5 and IL-13. K37 also improved cell infiltration in lung sections. Our results demonstrated that oral administration of K37 alleviated effectively the allergic responses in vivo. Thus, K37 can be a good source material and a promising candidate for prophylactic and therapeutic treatments of allergic diseases, like asthma.

Show MeSH
Related in: MedlinePlus