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Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

Meester I, Rosas-Taraco AG, Salinas-Carmona MC - PLoS ONE (2014)

Bottom Line: CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice.Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red.In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Nuevo León, México.

ABSTRACT
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

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Related in: MedlinePlus

Transferred CFSE-labeled macrophages (Mφ) and dendritic cells (DC) become lipid-laden.Macrophages (Mφ; in A) and dendritic cells (DC; in B) transferred at day 30 of a N. brasiliensis infection are 7 days later localized in the fibrotic ring (FR) of a microabscess (MA). Non-adherent spleen control cells (NACC; in C) transferred at day 30 of a N. brasiliensis infection are 7 days later localized outside of the FR and MA. Nile Red staining of lipid droplets is observed in transferred Mφ (D) and DC (E). Transferred cells are stained green by CFSE, lipid droplets are stained red by Nile Red, and nuclei are stained blue with DAPI.
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pone-0100064-g005: Transferred CFSE-labeled macrophages (Mφ) and dendritic cells (DC) become lipid-laden.Macrophages (Mφ; in A) and dendritic cells (DC; in B) transferred at day 30 of a N. brasiliensis infection are 7 days later localized in the fibrotic ring (FR) of a microabscess (MA). Non-adherent spleen control cells (NACC; in C) transferred at day 30 of a N. brasiliensis infection are 7 days later localized outside of the FR and MA. Nile Red staining of lipid droplets is observed in transferred Mφ (D) and DC (E). Transferred cells are stained green by CFSE, lipid droplets are stained red by Nile Red, and nuclei are stained blue with DAPI.

Mentions: The in vitro study revealed that BMDM and BMDC became foamy cells as both accumulated lipids into their cytoplasm after infection with N. brasiliensis, whereas uninfected BMDM and BMDC did not (fig. 4). CFSE-labeled BMDM and BMDC, transferred into 30-days lesions of experimental actinomycetoma induced by N. brasiliensis, could be traced 7 days later interspersed into the fibrotic ring of multilocular microabscesses, the typical site of foamy cells. The lipid droplet fluorophore Nile Red co-localized within the transferred cells, either BMDM or BMDC, as well as in recipient’s own cells that had accumulated lipid droplets (fig. 5). On the other hand, non-adherent control cells were localized out of fibrotic area and did not accumulate lipid droplets. Thus, we demonstrated that macrophages and DC can be differentiated into foamy cells, both in vitro and in vivo.


Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

Meester I, Rosas-Taraco AG, Salinas-Carmona MC - PLoS ONE (2014)

Transferred CFSE-labeled macrophages (Mφ) and dendritic cells (DC) become lipid-laden.Macrophages (Mφ; in A) and dendritic cells (DC; in B) transferred at day 30 of a N. brasiliensis infection are 7 days later localized in the fibrotic ring (FR) of a microabscess (MA). Non-adherent spleen control cells (NACC; in C) transferred at day 30 of a N. brasiliensis infection are 7 days later localized outside of the FR and MA. Nile Red staining of lipid droplets is observed in transferred Mφ (D) and DC (E). Transferred cells are stained green by CFSE, lipid droplets are stained red by Nile Red, and nuclei are stained blue with DAPI.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061056&req=5

pone-0100064-g005: Transferred CFSE-labeled macrophages (Mφ) and dendritic cells (DC) become lipid-laden.Macrophages (Mφ; in A) and dendritic cells (DC; in B) transferred at day 30 of a N. brasiliensis infection are 7 days later localized in the fibrotic ring (FR) of a microabscess (MA). Non-adherent spleen control cells (NACC; in C) transferred at day 30 of a N. brasiliensis infection are 7 days later localized outside of the FR and MA. Nile Red staining of lipid droplets is observed in transferred Mφ (D) and DC (E). Transferred cells are stained green by CFSE, lipid droplets are stained red by Nile Red, and nuclei are stained blue with DAPI.
Mentions: The in vitro study revealed that BMDM and BMDC became foamy cells as both accumulated lipids into their cytoplasm after infection with N. brasiliensis, whereas uninfected BMDM and BMDC did not (fig. 4). CFSE-labeled BMDM and BMDC, transferred into 30-days lesions of experimental actinomycetoma induced by N. brasiliensis, could be traced 7 days later interspersed into the fibrotic ring of multilocular microabscesses, the typical site of foamy cells. The lipid droplet fluorophore Nile Red co-localized within the transferred cells, either BMDM or BMDC, as well as in recipient’s own cells that had accumulated lipid droplets (fig. 5). On the other hand, non-adherent control cells were localized out of fibrotic area and did not accumulate lipid droplets. Thus, we demonstrated that macrophages and DC can be differentiated into foamy cells, both in vitro and in vivo.

Bottom Line: CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice.Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red.In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Nuevo León, México.

ABSTRACT
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

Show MeSH
Related in: MedlinePlus