Limits...
Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

Meester I, Rosas-Taraco AG, Salinas-Carmona MC - PLoS ONE (2014)

Bottom Line: CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice.Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red.In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Nuevo León, México.

ABSTRACT
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

Show MeSH

Related in: MedlinePlus

CFSE-labeling does not alter phenotype.A) BMDM and BMDC were fluorescent-labeled with 2 µM CFSE. B) Representative dot plots (1 out of 3 independent experiments) show that control (CTR) and CFSE-labeled macrophages (CFSE) had similar CD11b and CD11c expression profiles.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4061056&req=5

pone-0100064-g002: CFSE-labeling does not alter phenotype.A) BMDM and BMDC were fluorescent-labeled with 2 µM CFSE. B) Representative dot plots (1 out of 3 independent experiments) show that control (CTR) and CFSE-labeled macrophages (CFSE) had similar CD11b and CD11c expression profiles.

Mentions: Although CFSE labeling (fig. 2a) affected BMDM and BMDC morphology at the short term with cells rounding up during the first hours after labeling, the next day, viable cells had recovered typical morphology and adherence. The immunophenotype pattern was hardly altered between control and CFSE-labeled BMDM or BMDC (fig. 2b) and considered acceptable. Similarly, functionality was unaffected as verified by phagocytosis studies of harvested and reseeded CFSE-labeled and control BMDM, which had phagocytosis indices of 96.0% (±2.96) and 97.6% (±2.14), respectively; meanwhile CFSE-labeled and control BMDC had phagocytosis indices of 78.6% (±4.1) and 77.6% (±5.8), respectively (fig. 3).


Nocardia brasiliensis induces formation of foamy macrophages and dendritic cells in vitro and in vivo.

Meester I, Rosas-Taraco AG, Salinas-Carmona MC - PLoS ONE (2014)

CFSE-labeling does not alter phenotype.A) BMDM and BMDC were fluorescent-labeled with 2 µM CFSE. B) Representative dot plots (1 out of 3 independent experiments) show that control (CTR) and CFSE-labeled macrophages (CFSE) had similar CD11b and CD11c expression profiles.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061056&req=5

pone-0100064-g002: CFSE-labeling does not alter phenotype.A) BMDM and BMDC were fluorescent-labeled with 2 µM CFSE. B) Representative dot plots (1 out of 3 independent experiments) show that control (CTR) and CFSE-labeled macrophages (CFSE) had similar CD11b and CD11c expression profiles.
Mentions: Although CFSE labeling (fig. 2a) affected BMDM and BMDC morphology at the short term with cells rounding up during the first hours after labeling, the next day, viable cells had recovered typical morphology and adherence. The immunophenotype pattern was hardly altered between control and CFSE-labeled BMDM or BMDC (fig. 2b) and considered acceptable. Similarly, functionality was unaffected as verified by phagocytosis studies of harvested and reseeded CFSE-labeled and control BMDM, which had phagocytosis indices of 96.0% (±2.96) and 97.6% (±2.14), respectively; meanwhile CFSE-labeled and control BMDC had phagocytosis indices of 78.6% (±4.1) and 77.6% (±5.8), respectively (fig. 3).

Bottom Line: CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice.Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red.In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

View Article: PubMed Central - PubMed

Affiliation: Department of Immunology, Faculty of Medicine, Universidad Autónoma de Nuevo León, Nuevo León, México.

ABSTRACT
Foamy cells have been described in various infectious diseases, for example in actinomycetoma induced by Nocardia brasiliensis. These cells are generally considered to be macrophages, although they present dendritic cell (DC)-specific surface markers. In this study, we determined and confirmed the lineage of possible precursors of foamy cells in vitro and in vivo using an experimental actinomycetoma model in BALB/c mice. Bone marrow-derived macrophages (BMDM) or DC (BMDC) were infected in vitro with N. brasiliensis or labeled with carboxyfluorescein diacetate succinimidyl ester (CFSE). Both, macrophages and DC, differentiated into foamy cells after in vitro infection. CFSE-labeled BMDM or BMDC were tested for phagocytosis and CD11c/CD11b receptors markers expression before being transferred into the actinomycetoma lesion site of infected mice. In vivo studies showed that BMDM and BMDC were traced at the site where foamy cells are present in the experimental actinomycetoma. Interestingly, many of the transferred BMDM and BMDC were stained with the lipid-droplet fluorophore Nile Red. In conclusion, macrophages and DC cells can be differentiated into foamy cells in vitro and in vivo during N. brasiliensis infection.

Show MeSH
Related in: MedlinePlus