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Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.

Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L - PLoS ONE (2014)

Bottom Line: According to their urinary albumin-to-creatinine ratio (uACR), 107 patients with type 2 diabetes (eGFR >60 ml/min) were divided into normal albuminuria group (DN0 group, 38 cases), microalbuminuria group (DN1 group, 38 cases), and macroalbuminuria group (DN2 group, 31 cases), compared with matched healthy normal control group (NC group, 30 cases).Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting.Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

ABSTRACT

Aims: To investigate if nuclear NF-κB p65 expression in ex vivo isolated peripheral blood mononuclear cells correlates with urinary MCP-1 or RANTES and the severity of type 2 diabetic nephropathy.

Methods: According to their urinary albumin-to-creatinine ratio (uACR), 107 patients with type 2 diabetes (eGFR >60 ml/min) were divided into normal albuminuria group (DN0 group, 38 cases), microalbuminuria group (DN1 group, 38 cases), and macroalbuminuria group (DN2 group, 31 cases), compared with matched healthy normal control group (NC group, 30 cases). Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting. Real-time quantitative polymerase chain reaction was used to detect NF-κB p65 mRNA expression and ELISA assay was used to detect the levels of urinary MCP-1 and RANTES.

Results: Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group. In particular, the increase of nuclear NF-κB p65 protein and NF-κB p65 mRNA expressions, urinary MCP-1/Cr and RANTES/Cr all correlated with the severity of type 2 diabetic nephropathy as indicated by the increase in uACR. Pearson correlation analysis indicated that both urinary MCP-1/Cr and RANTES/Cr were positively correlated with nuclear NF-κB p65 protein or NF-κB p65 mRNA levels. Stepwise multiple regression analysis showed that nuclear NF-κB p65 protein or NF-κB p65 mRNA was an independent variable for urinary MCP-1/Cr, and MCP-1/Cr and RANTES/Cr were two independent variables for uACR.

Conclusion: Our research demonstrates that nuclear NF-κB p65 protein and mRNA expressions in ex vivo isolated peripheral blood mononuclear cells well correlate with urinary MCP-1/Cr, RANTES/Cr and the severity of type 2 diabetic nephropathy.

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Nuclear NF-κB p65 protein level in peripheral blood mononuclear cells determined by western blotting.Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.
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pone-0099633-g001: Nuclear NF-κB p65 protein level in peripheral blood mononuclear cells determined by western blotting.Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.

Mentions: As shown in the western blotting results (Figure 1), the nuclear NF-κB p65 expression of peripheral blood mononuclear cell in NC group was the lowest, with gradual increase in DN0, DN1 and DN2 groups, respectively, and with DN2 group being the highest. All differences were statistically significant compared with NC group. Notably, NF-κB p65 protein expression was significantly higher in DN2 and DN1 groups than that in DN0 group (Figure 1).


Nuclear NF-κB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.

Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L - PLoS ONE (2014)

Nuclear NF-κB p65 protein level in peripheral blood mononuclear cells determined by western blotting.Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4061032&req=5

pone-0099633-g001: Nuclear NF-κB p65 protein level in peripheral blood mononuclear cells determined by western blotting.Note: Compared with NC group **: P<0.01; compared with DN0 group ##: P<0.01.
Mentions: As shown in the western blotting results (Figure 1), the nuclear NF-κB p65 expression of peripheral blood mononuclear cell in NC group was the lowest, with gradual increase in DN0, DN1 and DN2 groups, respectively, and with DN2 group being the highest. All differences were statistically significant compared with NC group. Notably, NF-κB p65 protein expression was significantly higher in DN2 and DN1 groups than that in DN0 group (Figure 1).

Bottom Line: According to their urinary albumin-to-creatinine ratio (uACR), 107 patients with type 2 diabetes (eGFR >60 ml/min) were divided into normal albuminuria group (DN0 group, 38 cases), microalbuminuria group (DN1 group, 38 cases), and macroalbuminuria group (DN2 group, 31 cases), compared with matched healthy normal control group (NC group, 30 cases).Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting.Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group.

View Article: PubMed Central - PubMed

Affiliation: Department of Nephrology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.

ABSTRACT

Aims: To investigate if nuclear NF-κB p65 expression in ex vivo isolated peripheral blood mononuclear cells correlates with urinary MCP-1 or RANTES and the severity of type 2 diabetic nephropathy.

Methods: According to their urinary albumin-to-creatinine ratio (uACR), 107 patients with type 2 diabetes (eGFR >60 ml/min) were divided into normal albuminuria group (DN0 group, 38 cases), microalbuminuria group (DN1 group, 38 cases), and macroalbuminuria group (DN2 group, 31 cases), compared with matched healthy normal control group (NC group, 30 cases). Nuclear NF-κB p65 protein expression levels in peripheral blood mononuclear cells were detected by western blotting. Real-time quantitative polymerase chain reaction was used to detect NF-κB p65 mRNA expression and ELISA assay was used to detect the levels of urinary MCP-1 and RANTES.

Results: Nuclear NF-κB p65 protein and NF-κB p65 mRNA expression levels in peripheral blood mononuclear cells, urinary MCP-1/Cr and RANTES/Cr were all significantly higher in all diabetes groups as compared with NC group. In particular, the increase of nuclear NF-κB p65 protein and NF-κB p65 mRNA expressions, urinary MCP-1/Cr and RANTES/Cr all correlated with the severity of type 2 diabetic nephropathy as indicated by the increase in uACR. Pearson correlation analysis indicated that both urinary MCP-1/Cr and RANTES/Cr were positively correlated with nuclear NF-κB p65 protein or NF-κB p65 mRNA levels. Stepwise multiple regression analysis showed that nuclear NF-κB p65 protein or NF-κB p65 mRNA was an independent variable for urinary MCP-1/Cr, and MCP-1/Cr and RANTES/Cr were two independent variables for uACR.

Conclusion: Our research demonstrates that nuclear NF-κB p65 protein and mRNA expressions in ex vivo isolated peripheral blood mononuclear cells well correlate with urinary MCP-1/Cr, RANTES/Cr and the severity of type 2 diabetic nephropathy.

Show MeSH
Related in: MedlinePlus