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Decreased expression of C10orf10 and its prognostic significance in human breast cancer.

Deng J, Dong Y, Li C, Zuo W, Meng G, Xu C, Li J - PLoS ONE (2014)

Bottom Line: Here, we identified C10orf10 as a novel differentially expression gene using expression microarray screening.Kaplan-Meier analysis indicated that patients with low C10orf10 expression showed a poorer prognosis both in mRNA (n = 1115, P = 0.0013) and protein (n = 100, P = 0.003) levels.Further analysis revealed that low expression of C10orf10 was an unfavorable factor for the prognosis of the patients who were luminal A, luminal B, Her2+ subtypes, at histological grade 2, lymph node negative and ER positive.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing, China; Cadre's Sanatorium, Third Military Medical University, Chongqing, China.

ABSTRACT
Breast cancer is a common malignant tumor, which severely threatens the health of women with an increasing incidence in many countries. Here, we identified C10orf10 as a novel differentially expression gene using expression microarray screening. The expression analysis indicated that C10orf10 was frequently decreased in human breast cancers compared to noncancerous breast tissues (81/95, P = 0.0063). Kaplan-Meier analysis indicated that patients with low C10orf10 expression showed a poorer prognosis both in mRNA (n = 1115, P = 0.0013) and protein (n = 100, P = 0.003) levels. Univariate and multivariate analysis showed that the C10orf10 expression was an independent prognostic factor for overall survival of breast cancer patients. Further analysis revealed that low expression of C10orf10 was an unfavorable factor for the prognosis of the patients who were luminal A, luminal B, Her2+ subtypes, at histological grade 2, lymph node negative and ER positive. Our data provided the first evidence that C10orf10 expression was frequently decreased in breast cancer tissues, and low expression of C10orf10 may be an important prognostic factor for poorer survival time of breast cancer patients.

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Low C10orf10 expression is correlated with shorter survival in BC patients.(A) Survival analysis of C10orf10 protein expression in 100 BC patients by Kaplan-Meier survival curve. Tissue array analysis was performed in 100 cases of patients with the survival information. The patients with low expression had a poorer overall survival than those with high expression; HR, hazard ratio; low, staining negative and weak; high, staining moderate and strong. (B) Survival analysis of C10orf10 expression in 100 BC patients by multivariate Cox regression. The C10orf10 expression is an independent prognostic factor. (C) Kaplan-Meir survival analysis of C10orf10 mRNA in 1115 BC patients with Kaplan-Meier Plotter (http://kmplot.com/analysis/index). The overall survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1086; Expression range of the probe was 143–3881. (D) Kaplan-Meir survival analysis of C10orf10 mRNA in 3455 BC patients with Kaplan-Meier Plotter. The relapse-free survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1088; Expression range of the probe was 32–5515.
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pone-0099730-g003: Low C10orf10 expression is correlated with shorter survival in BC patients.(A) Survival analysis of C10orf10 protein expression in 100 BC patients by Kaplan-Meier survival curve. Tissue array analysis was performed in 100 cases of patients with the survival information. The patients with low expression had a poorer overall survival than those with high expression; HR, hazard ratio; low, staining negative and weak; high, staining moderate and strong. (B) Survival analysis of C10orf10 expression in 100 BC patients by multivariate Cox regression. The C10orf10 expression is an independent prognostic factor. (C) Kaplan-Meir survival analysis of C10orf10 mRNA in 1115 BC patients with Kaplan-Meier Plotter (http://kmplot.com/analysis/index). The overall survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1086; Expression range of the probe was 143–3881. (D) Kaplan-Meir survival analysis of C10orf10 mRNA in 3455 BC patients with Kaplan-Meier Plotter. The relapse-free survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1088; Expression range of the probe was 32–5515.

Mentions: Decreased expression of C10orf10 in BCs suggests that it may be associated with BC development. To investigate the correlation between C10orf10 expression and survival of BC patients, we scored the specific staining of the immunohistochemical TMA containing 100 BC patients by intensity and percentage of positive staining, combining negative with low, and mediate with high expression to obtain two groups, low and high, respectively. Survival analysis by Kaplan-Meier and log rank test demonstrated that the patients with high expression of C10orf10 protein had longer OS time than these with low expression (P = 0.003) (Figure 3A). To avoid the influence caused by univariate analysis, the C10orf10 expression as well as other parameters was examined in multivariate Cox regression analysis (adjusted for age, stage, grade, tumor size and lymph node). The C10orf10 protein expression was found to may be an independent prognostic factor (hazard ratio (HR)  = 0.418, P = 0.007) in addition to age (HR = 1.044, P = 0.012) and lymph node (HR = 1.089, P = 0.017) (Figure 3B, Table 1). When investigating the association between C10orf10 expression and clinicopathologic features of BC patients, there were no significant correlations between C10orf10 expression and age, stage, grade and tumor size (Table 2). However, there was a significant correlation between C10orf10 expression and lymph node status (P = 0.045) (Table 2).


Decreased expression of C10orf10 and its prognostic significance in human breast cancer.

Deng J, Dong Y, Li C, Zuo W, Meng G, Xu C, Li J - PLoS ONE (2014)

Low C10orf10 expression is correlated with shorter survival in BC patients.(A) Survival analysis of C10orf10 protein expression in 100 BC patients by Kaplan-Meier survival curve. Tissue array analysis was performed in 100 cases of patients with the survival information. The patients with low expression had a poorer overall survival than those with high expression; HR, hazard ratio; low, staining negative and weak; high, staining moderate and strong. (B) Survival analysis of C10orf10 expression in 100 BC patients by multivariate Cox regression. The C10orf10 expression is an independent prognostic factor. (C) Kaplan-Meir survival analysis of C10orf10 mRNA in 1115 BC patients with Kaplan-Meier Plotter (http://kmplot.com/analysis/index). The overall survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1086; Expression range of the probe was 143–3881. (D) Kaplan-Meir survival analysis of C10orf10 mRNA in 3455 BC patients with Kaplan-Meier Plotter. The relapse-free survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1088; Expression range of the probe was 32–5515.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4061027&req=5

pone-0099730-g003: Low C10orf10 expression is correlated with shorter survival in BC patients.(A) Survival analysis of C10orf10 protein expression in 100 BC patients by Kaplan-Meier survival curve. Tissue array analysis was performed in 100 cases of patients with the survival information. The patients with low expression had a poorer overall survival than those with high expression; HR, hazard ratio; low, staining negative and weak; high, staining moderate and strong. (B) Survival analysis of C10orf10 expression in 100 BC patients by multivariate Cox regression. The C10orf10 expression is an independent prognostic factor. (C) Kaplan-Meir survival analysis of C10orf10 mRNA in 1115 BC patients with Kaplan-Meier Plotter (http://kmplot.com/analysis/index). The overall survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1086; Expression range of the probe was 143–3881. (D) Kaplan-Meir survival analysis of C10orf10 mRNA in 3455 BC patients with Kaplan-Meier Plotter. The relapse-free survival was longer in the C10orf10 high expression group than in the C10orf10 low expression group. Auto select best cutoff was chosen in the analysis; Cutoff value used was 1088; Expression range of the probe was 32–5515.
Mentions: Decreased expression of C10orf10 in BCs suggests that it may be associated with BC development. To investigate the correlation between C10orf10 expression and survival of BC patients, we scored the specific staining of the immunohistochemical TMA containing 100 BC patients by intensity and percentage of positive staining, combining negative with low, and mediate with high expression to obtain two groups, low and high, respectively. Survival analysis by Kaplan-Meier and log rank test demonstrated that the patients with high expression of C10orf10 protein had longer OS time than these with low expression (P = 0.003) (Figure 3A). To avoid the influence caused by univariate analysis, the C10orf10 expression as well as other parameters was examined in multivariate Cox regression analysis (adjusted for age, stage, grade, tumor size and lymph node). The C10orf10 protein expression was found to may be an independent prognostic factor (hazard ratio (HR)  = 0.418, P = 0.007) in addition to age (HR = 1.044, P = 0.012) and lymph node (HR = 1.089, P = 0.017) (Figure 3B, Table 1). When investigating the association between C10orf10 expression and clinicopathologic features of BC patients, there were no significant correlations between C10orf10 expression and age, stage, grade and tumor size (Table 2). However, there was a significant correlation between C10orf10 expression and lymph node status (P = 0.045) (Table 2).

Bottom Line: Here, we identified C10orf10 as a novel differentially expression gene using expression microarray screening.Kaplan-Meier analysis indicated that patients with low C10orf10 expression showed a poorer prognosis both in mRNA (n = 1115, P = 0.0013) and protein (n = 100, P = 0.003) levels.Further analysis revealed that low expression of C10orf10 was an unfavorable factor for the prognosis of the patients who were luminal A, luminal B, Her2+ subtypes, at histological grade 2, lymph node negative and ER positive.

View Article: PubMed Central - PubMed

Affiliation: Department of Oncology, Southwest Hospital, Third Military Medical University, Chongqing, China; Cadre's Sanatorium, Third Military Medical University, Chongqing, China.

ABSTRACT
Breast cancer is a common malignant tumor, which severely threatens the health of women with an increasing incidence in many countries. Here, we identified C10orf10 as a novel differentially expression gene using expression microarray screening. The expression analysis indicated that C10orf10 was frequently decreased in human breast cancers compared to noncancerous breast tissues (81/95, P = 0.0063). Kaplan-Meier analysis indicated that patients with low C10orf10 expression showed a poorer prognosis both in mRNA (n = 1115, P = 0.0013) and protein (n = 100, P = 0.003) levels. Univariate and multivariate analysis showed that the C10orf10 expression was an independent prognostic factor for overall survival of breast cancer patients. Further analysis revealed that low expression of C10orf10 was an unfavorable factor for the prognosis of the patients who were luminal A, luminal B, Her2+ subtypes, at histological grade 2, lymph node negative and ER positive. Our data provided the first evidence that C10orf10 expression was frequently decreased in breast cancer tissues, and low expression of C10orf10 may be an important prognostic factor for poorer survival time of breast cancer patients.

Show MeSH
Related in: MedlinePlus