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Isolation of intraflagellar transport trains.

Mencarelli C, Mitchell A, Leoncini R, Rosenbaum J, Lupetti P - Cytoskeleton (Hoboken) (2013)

Bottom Line: Moreover, the particles forming isolated IFT trains are structurally similar to the individual particles found in the ∼17S gradient peak.Our results provide the first direct evidence that ∼17S particles do indeed compose the IFT trains.The paper also represents the first isolation of the IFT trains, and opens new possibilities for higher resolution studies on their structure and how particles are attached to each other to form the particle trains.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

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Thin sections of the gradient pellet.A. Low magnification view. IFT particles appear as electrondense particles, some of which are here indicated by white arrowheads. Particles often associate to form large aggregates (as the one containing the particles indicated by the white arrowheads), or are associated with membrane vesicles (indicated by the black arrows). B. Higher magnification views of short, double row arrays of particles, which are indicated by brackets. The arrows indicate the interconnecting links between adjacent particles. The arrowhead indicate a double row array of particles showing a more compact organization. C. In some sections, particles appear to possess a central cleft (arrows).
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fig07: Thin sections of the gradient pellet.A. Low magnification view. IFT particles appear as electrondense particles, some of which are here indicated by white arrowheads. Particles often associate to form large aggregates (as the one containing the particles indicated by the white arrowheads), or are associated with membrane vesicles (indicated by the black arrows). B. Higher magnification views of short, double row arrays of particles, which are indicated by brackets. The arrows indicate the interconnecting links between adjacent particles. The arrowhead indicate a double row array of particles showing a more compact organization. C. In some sections, particles appear to possess a central cleft (arrows).

Mentions: To confirm the results obtained by negative staining of the IFT particles recovered in the gradient pellet, this pellet was also analyzed after plastic embedding and thin-sectioning. IFT particles appear in thin sections as electrondense particles, either arranged to form aggregates of variable dimensions or associated with membrane vesicles (Fig. 7A). Double rows of particles could be observed (brackets in Fig. 7B). Within this arrangement, particles may be disposed in a quite loose array, where they appear to be connected by links (arrows) or may be closer to each other forming a compact array (arrowhead). Depending on their orientation and on the plane of sectioning, some particles appear to possess a small cleft on one side (arrowheads in Fig. 7C).


Isolation of intraflagellar transport trains.

Mencarelli C, Mitchell A, Leoncini R, Rosenbaum J, Lupetti P - Cytoskeleton (Hoboken) (2013)

Thin sections of the gradient pellet.A. Low magnification view. IFT particles appear as electrondense particles, some of which are here indicated by white arrowheads. Particles often associate to form large aggregates (as the one containing the particles indicated by the white arrowheads), or are associated with membrane vesicles (indicated by the black arrows). B. Higher magnification views of short, double row arrays of particles, which are indicated by brackets. The arrows indicate the interconnecting links between adjacent particles. The arrowhead indicate a double row array of particles showing a more compact organization. C. In some sections, particles appear to possess a central cleft (arrows).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060975&req=5

fig07: Thin sections of the gradient pellet.A. Low magnification view. IFT particles appear as electrondense particles, some of which are here indicated by white arrowheads. Particles often associate to form large aggregates (as the one containing the particles indicated by the white arrowheads), or are associated with membrane vesicles (indicated by the black arrows). B. Higher magnification views of short, double row arrays of particles, which are indicated by brackets. The arrows indicate the interconnecting links between adjacent particles. The arrowhead indicate a double row array of particles showing a more compact organization. C. In some sections, particles appear to possess a central cleft (arrows).
Mentions: To confirm the results obtained by negative staining of the IFT particles recovered in the gradient pellet, this pellet was also analyzed after plastic embedding and thin-sectioning. IFT particles appear in thin sections as electrondense particles, either arranged to form aggregates of variable dimensions or associated with membrane vesicles (Fig. 7A). Double rows of particles could be observed (brackets in Fig. 7B). Within this arrangement, particles may be disposed in a quite loose array, where they appear to be connected by links (arrows) or may be closer to each other forming a compact array (arrowhead). Depending on their orientation and on the plane of sectioning, some particles appear to possess a small cleft on one side (arrowheads in Fig. 7C).

Bottom Line: Moreover, the particles forming isolated IFT trains are structurally similar to the individual particles found in the ∼17S gradient peak.Our results provide the first direct evidence that ∼17S particles do indeed compose the IFT trains.The paper also represents the first isolation of the IFT trains, and opens new possibilities for higher resolution studies on their structure and how particles are attached to each other to form the particle trains.

View Article: PubMed Central - PubMed

Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.

Show MeSH
Related in: MedlinePlus