Isolation of intraflagellar transport trains.
Bottom Line: Moreover, the particles forming isolated IFT trains are structurally similar to the individual particles found in the ∼17S gradient peak.Our results provide the first direct evidence that ∼17S particles do indeed compose the IFT trains.The paper also represents the first isolation of the IFT trains, and opens new possibilities for higher resolution studies on their structure and how particles are attached to each other to form the particle trains.
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.Show MeSH
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Mentions: To confirm the results obtained by negative staining of the IFT particles recovered in the gradient pellet, this pellet was also analyzed after plastic embedding and thin-sectioning. IFT particles appear in thin sections as electrondense particles, either arranged to form aggregates of variable dimensions or associated with membrane vesicles (Fig. 7A). Double rows of particles could be observed (brackets in Fig. 7B). Within this arrangement, particles may be disposed in a quite loose array, where they appear to be connected by links (arrows) or may be closer to each other forming a compact array (arrowhead). Depending on their orientation and on the plane of sectioning, some particles appear to possess a small cleft on one side (arrowheads in Fig. 7C).
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.