Isolation of intraflagellar transport trains.
Bottom Line: Moreover, the particles forming isolated IFT trains are structurally similar to the individual particles found in the ∼17S gradient peak.Our results provide the first direct evidence that ∼17S particles do indeed compose the IFT trains.The paper also represents the first isolation of the IFT trains, and opens new possibilities for higher resolution studies on their structure and how particles are attached to each other to form the particle trains.
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.Show MeSH
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Mentions: Short regions showing an almost regular architecture consisting of two parallel strings of particles could be frequently observed, even within larger aggregates (see brackets in Fig. 5). Such a double arrangement of particles is a peculiar feature of the architecture previously described in anterograde IFT trains, where particles are interconnected by a double series of oblique links [Pigino et al., 2009]. Oblique links could be discerned in either linear or double strings particle arrays, as exemplified in Fig. 6, where they are compared with the tomogram produced by Pigino et al.
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.