Isolation of intraflagellar transport trains.
Bottom Line: Moreover, the particles forming isolated IFT trains are structurally similar to the individual particles found in the ∼17S gradient peak.Our results provide the first direct evidence that ∼17S particles do indeed compose the IFT trains.The paper also represents the first isolation of the IFT trains, and opens new possibilities for higher resolution studies on their structure and how particles are attached to each other to form the particle trains.
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.Show MeSH
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Mentions: IFT immunolabeling was specifically associated with the arrays of particles in the gradient pellet (Fig. 5A). When observed at a higher magnification, strings of particles appear to be labeled by both IFT139 (white arrows in Fig. 5B) and IFT46 (black arrows in Fig. 5B) antibodies. This finding clearly indicates that both complex A and complex B are present in the same string of particles.
Affiliation: Department of Life Sciences, University of Siena, Via Aldo Moro 2, 53100, Siena, Italy.