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The genome sequence of the highly acetic acid-tolerant Zygosaccharomyces bailii-derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant.

Mira NP, Münsterkötter M, Dias-Valada F, Santos J, Palma M, Roque FC, Guerreiro JF, Rodrigues F, Sousa MJ, Leão C, Güldener U, Sá-Correia I - DNA Res. (2014)

Bottom Line: Annotation of ISA1307 genome includes 4385 duplicated genes (∼ 90% of the total number of predicted genes) and 1155 predicted single-copy genes.The functional categories including a higher number of genes are 'Metabolism and generation of energy', 'Protein folding, modification and targeting' and 'Biogenesis of cellular components'.The knowledge of the genome sequence of the ISA1307 strain is expected to contribute to accelerate systems-level understanding of stress resistance mechanisms in Z. bailii and to inspire and guide novel biotechnological applications of this yeast species/strain in fermentation processes, given its high resilience to acidic stress.

View Article: PubMed Central - PubMed

Affiliation: IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Department of Bioengineering, Universidade de Lisboa, Avenida Rovisco Pais, Lisbon 1049-001, Portugal.

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Frequency of putative domains in the ISA1307 predicted proteome. The proteins predicted to be encoded by the ISA1307 genome were searched for putative conserved domains using InterProScan and domains found present in >50 predicted proteins were selected and are highlighted.
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DST058F4: Frequency of putative domains in the ISA1307 predicted proteome. The proteins predicted to be encoded by the ISA1307 genome were searched for putative conserved domains using InterProScan and domains found present in >50 predicted proteins were selected and are highlighted.

Mentions: The function of the 9931 gene loci predicted to be encoded by the genome of the ISA1307 strain was clustered according to their physiological function using the FunCatDB functional catalogue48 (Fig. 3). The highest number of genes were found in the functional classes of ‘Metabolism and generation of energy’ (35% of the total of predicted genes), ‘Protein folding, modification and targeting’ (25% of the predicted genes) and ‘Biogenesis of cellular components’ (21% of the predicted genes) (Fig. 3). The functional categorization of the ISA1307 genome is, in general, similar to the one obtained for S. cerevisiae S288c or Z. rouxii CBS732 genomes (Supplementary Fig. S1). Genes encoding transposable elements were found to be very scarce in the ISA1307 genome (Supplementary Fig. S1). The more abundant motifs found in the proteins predicted to be encoded by the ISA1307 genome were: (i) the WD40/YTVN motif, present in signal transducing G-proteins or in actin-interacting proteins, (ii) kinases-associated motifs, (iii) motifs present in NADP+-binding enzymes, (iv) the armadillo motif, found in protein phosphatases and in initiation translation factors and (v) signature motifs of transporters of the MFS (Fig. 4). These motifs were also the more abundant motifs found in S. cerevisiae S288c or Z. rouxii CBS732 proteomes (results not shown).Figure 3.


The genome sequence of the highly acetic acid-tolerant Zygosaccharomyces bailii-derived interspecies hybrid strain ISA1307, isolated from a sparkling wine plant.

Mira NP, Münsterkötter M, Dias-Valada F, Santos J, Palma M, Roque FC, Guerreiro JF, Rodrigues F, Sousa MJ, Leão C, Güldener U, Sá-Correia I - DNA Res. (2014)

Frequency of putative domains in the ISA1307 predicted proteome. The proteins predicted to be encoded by the ISA1307 genome were searched for putative conserved domains using InterProScan and domains found present in >50 predicted proteins were selected and are highlighted.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060950&req=5

DST058F4: Frequency of putative domains in the ISA1307 predicted proteome. The proteins predicted to be encoded by the ISA1307 genome were searched for putative conserved domains using InterProScan and domains found present in >50 predicted proteins were selected and are highlighted.
Mentions: The function of the 9931 gene loci predicted to be encoded by the genome of the ISA1307 strain was clustered according to their physiological function using the FunCatDB functional catalogue48 (Fig. 3). The highest number of genes were found in the functional classes of ‘Metabolism and generation of energy’ (35% of the total of predicted genes), ‘Protein folding, modification and targeting’ (25% of the predicted genes) and ‘Biogenesis of cellular components’ (21% of the predicted genes) (Fig. 3). The functional categorization of the ISA1307 genome is, in general, similar to the one obtained for S. cerevisiae S288c or Z. rouxii CBS732 genomes (Supplementary Fig. S1). Genes encoding transposable elements were found to be very scarce in the ISA1307 genome (Supplementary Fig. S1). The more abundant motifs found in the proteins predicted to be encoded by the ISA1307 genome were: (i) the WD40/YTVN motif, present in signal transducing G-proteins or in actin-interacting proteins, (ii) kinases-associated motifs, (iii) motifs present in NADP+-binding enzymes, (iv) the armadillo motif, found in protein phosphatases and in initiation translation factors and (v) signature motifs of transporters of the MFS (Fig. 4). These motifs were also the more abundant motifs found in S. cerevisiae S288c or Z. rouxii CBS732 proteomes (results not shown).Figure 3.

Bottom Line: Annotation of ISA1307 genome includes 4385 duplicated genes (∼ 90% of the total number of predicted genes) and 1155 predicted single-copy genes.The functional categories including a higher number of genes are 'Metabolism and generation of energy', 'Protein folding, modification and targeting' and 'Biogenesis of cellular components'.The knowledge of the genome sequence of the ISA1307 strain is expected to contribute to accelerate systems-level understanding of stress resistance mechanisms in Z. bailii and to inspire and guide novel biotechnological applications of this yeast species/strain in fermentation processes, given its high resilience to acidic stress.

View Article: PubMed Central - PubMed

Affiliation: IBB-Institute for Biotechnology and Bioengineering, Center for Biological and Chemical Engineering, Instituto Superior Técnico, Department of Bioengineering, Universidade de Lisboa, Avenida Rovisco Pais, Lisbon 1049-001, Portugal.

Show MeSH
Related in: MedlinePlus