Limits...
Comparative genome analysis of the closely related Synechocystis strains PCC 6714 and PCC 6803.

Kopf M, Klähn S, Pade N, Weingärtner C, Hagemann M, Voß B, Hess WR - DNA Res. (2014)

Bottom Line: PCC 6714.Genome information for Synechocystis sp.PCC 6714 has been deposited in Genbank (accession no AMZV01000000).

View Article: PubMed Central - PubMed

Affiliation: Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.

Show MeSH

Related in: MedlinePlus

Comparative genome analysis reveals the absence of the genes encoding the glucosylglycerol transport system (Ggt). (A) Genomic region encompassing the ggtA gene in Synechocystis 6803 and of the corresponding region in Synechocystis 6714. (B) Genomic region encompassing the ggtBCD operon. Apart from Ggt, both loci are well conserved (protein identity scores of ca. 90%). (C) Occurrence of mRNAs for ggtA, ggtBCD, and ggpS in salt-treated cells of both strains. For ggpS, salt-dependent expression was observed in both strains, whereas mRNAs for ggtA and ggtBCD were not detected in strain 6714.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4060947&req=5

DST055F5: Comparative genome analysis reveals the absence of the genes encoding the glucosylglycerol transport system (Ggt). (A) Genomic region encompassing the ggtA gene in Synechocystis 6803 and of the corresponding region in Synechocystis 6714. (B) Genomic region encompassing the ggtBCD operon. Apart from Ggt, both loci are well conserved (protein identity scores of ca. 90%). (C) Occurrence of mRNAs for ggtA, ggtBCD, and ggpS in salt-treated cells of both strains. For ggpS, salt-dependent expression was observed in both strains, whereas mRNAs for ggtA and ggtBCD were not detected in strain 6714.

Mentions: Another four genes, which were not found in the Synechocystis 6714 genome, are the ggtABCD genes encoding a transport system for the (re-)uptake of the compatible solute glucosylglycerol.43,44 Apart from that, the loci adjacent to ggtA or ggtBCD in Synechocystis 6803 are conserved in the genome of Synechocystis 6714 (Fig. 5A and B). To verify the absence of Ggt, Northern hybridization with 32P-labelled probes specific for ggtA or ggtBCD was performed with RNA from salt-treated cells. As expected, no mRNA was detected in salt-treated cells of strain 6714, whereas the expression level of the ggt genes correlated with the external salinity in Synechocystis 6803 (Fig. 5C). Moreover, the relative abundance of the mRNA for ggpS, the gene encoding the key enzyme of glucosylglycycerol synthesis, the main compatible solute in these two strains, was measured and revealed its salt-dependent expression in Synechocystis 6714 (Fig. 5C), similar to the well-characterized situation in Synechocystis 6803.45 These results further substantiated that, even though the 6714 genome is not completely finished, the lack of certain genes correlates to physiological differences.Figure 5.


Comparative genome analysis of the closely related Synechocystis strains PCC 6714 and PCC 6803.

Kopf M, Klähn S, Pade N, Weingärtner C, Hagemann M, Voß B, Hess WR - DNA Res. (2014)

Comparative genome analysis reveals the absence of the genes encoding the glucosylglycerol transport system (Ggt). (A) Genomic region encompassing the ggtA gene in Synechocystis 6803 and of the corresponding region in Synechocystis 6714. (B) Genomic region encompassing the ggtBCD operon. Apart from Ggt, both loci are well conserved (protein identity scores of ca. 90%). (C) Occurrence of mRNAs for ggtA, ggtBCD, and ggpS in salt-treated cells of both strains. For ggpS, salt-dependent expression was observed in both strains, whereas mRNAs for ggtA and ggtBCD were not detected in strain 6714.
© Copyright Policy - creative-commons
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060947&req=5

DST055F5: Comparative genome analysis reveals the absence of the genes encoding the glucosylglycerol transport system (Ggt). (A) Genomic region encompassing the ggtA gene in Synechocystis 6803 and of the corresponding region in Synechocystis 6714. (B) Genomic region encompassing the ggtBCD operon. Apart from Ggt, both loci are well conserved (protein identity scores of ca. 90%). (C) Occurrence of mRNAs for ggtA, ggtBCD, and ggpS in salt-treated cells of both strains. For ggpS, salt-dependent expression was observed in both strains, whereas mRNAs for ggtA and ggtBCD were not detected in strain 6714.
Mentions: Another four genes, which were not found in the Synechocystis 6714 genome, are the ggtABCD genes encoding a transport system for the (re-)uptake of the compatible solute glucosylglycerol.43,44 Apart from that, the loci adjacent to ggtA or ggtBCD in Synechocystis 6803 are conserved in the genome of Synechocystis 6714 (Fig. 5A and B). To verify the absence of Ggt, Northern hybridization with 32P-labelled probes specific for ggtA or ggtBCD was performed with RNA from salt-treated cells. As expected, no mRNA was detected in salt-treated cells of strain 6714, whereas the expression level of the ggt genes correlated with the external salinity in Synechocystis 6803 (Fig. 5C). Moreover, the relative abundance of the mRNA for ggpS, the gene encoding the key enzyme of glucosylglycycerol synthesis, the main compatible solute in these two strains, was measured and revealed its salt-dependent expression in Synechocystis 6714 (Fig. 5C), similar to the well-characterized situation in Synechocystis 6803.45 These results further substantiated that, even though the 6714 genome is not completely finished, the lack of certain genes correlates to physiological differences.Figure 5.

Bottom Line: PCC 6714.Genome information for Synechocystis sp.PCC 6714 has been deposited in Genbank (accession no AMZV01000000).

View Article: PubMed Central - PubMed

Affiliation: Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.

Show MeSH
Related in: MedlinePlus