Comparative genome analysis of the closely related Synechocystis strains PCC 6714 and PCC 6803.
Bottom Line: PCC 6714.Genome information for Synechocystis sp.PCC 6714 has been deposited in Genbank (accession no AMZV01000000).
Affiliation: Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.Show MeSH
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Mentions: Another four genes, which were not found in the Synechocystis 6714 genome, are the ggtABCD genes encoding a transport system for the (re-)uptake of the compatible solute glucosylglycerol.43,44 Apart from that, the loci adjacent to ggtA or ggtBCD in Synechocystis 6803 are conserved in the genome of Synechocystis 6714 (Fig. 5A and B). To verify the absence of Ggt, Northern hybridization with 32P-labelled probes specific for ggtA or ggtBCD was performed with RNA from salt-treated cells. As expected, no mRNA was detected in salt-treated cells of strain 6714, whereas the expression level of the ggt genes correlated with the external salinity in Synechocystis 6803 (Fig. 5C). Moreover, the relative abundance of the mRNA for ggpS, the gene encoding the key enzyme of glucosylglycycerol synthesis, the main compatible solute in these two strains, was measured and revealed its salt-dependent expression in Synechocystis 6714 (Fig. 5C), similar to the well-characterized situation in Synechocystis 6803.45 These results further substantiated that, even though the 6714 genome is not completely finished, the lack of certain genes correlates to physiological differences.Figure 5.
Affiliation: Genetics and Experimental Bioinformatics, Faculty of Biology, University of Freiburg, Schänzlestr. 1, D-79104 Freiburg, Germany.