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The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans.

Kheradmand E, Rafii F, Yazdi MH, Sepahi AA, Shahverdi AR, Oveisi MR - Daru (2014)

Bottom Line: The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles.Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. shahverd@tums.ac.ir.

ABSTRACT

Background: Lactic acid bacteria are considered important probiotics for prevention of some infections. The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.

Methods: Lactobacillus plantarum and L. johnsonii cells, grown in the presence and absence of selenium dioxide, and their cell-free spent culture media were tested for antifungal activity against C. albicans ATCC 14053 by a hole-plate diffusion method and a time-kill assay.

Results: Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles. The cell-free spent culture media, from both Lactobacillus species that had been grown with selenium dioxide for 48 h, showed enhanced antifungal activity against C. albicans. Enhanced antifungal activity of cell biomass against C. albicans was also observed in cultures grown with selenium dioxide.

Conclusions: Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

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Viability of C. albicans after incubation in culture supernatants of L. johnsonii or L. plantarum, grown with or without selenium dioxide.
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Figure 3: Viability of C. albicans after incubation in culture supernatants of L. johnsonii or L. plantarum, grown with or without selenium dioxide.

Mentions: The anti-Candida effects of the supernatants from L. plantarum and L. johnsonii, grown with and without selenium dioxide, were compared by measuring the zone of inhibition formed around each of the samples applied to the plates in the hole-plate diffusion method (Table 1). Negligible inhibition zones were observed on the plates containing culture supernatants of either of the species grown without selenium dioxide or sterile MRS broth supplemented with selenium dioxide (200 mg/l). Culture supernatants from L. plantarum and L. johnsonii grown with selenium dioxide for 48, 72, and 96 h showed potent anti-Candida activity and inhibited growth. The maximum antifungal activity was observed in 72 h cultures (Table 1). The time-kill assay, measuring the effect of culture supernatants of each strain, grown with or without selenium dioxide, on the viability of C. albicans, confirmed the increase in the antifungal activity of the strains grown with selenium dioxide (Figure 3). Whereas incubation of C. albicans with the supernatants of all cultures decreased viability, the number of surviving C. albicans cells substantially decreased when incubated with the supernatants of cultures grown for 72 h with selenium dioxide. The difference in the effect, which was observed even after 0.5 h incubation, increased with time. No viable C. albicans was present after 4 h incubation with culture supernatants of either species grown with selenium dioxide, but some viable C. albicans cells were present even after 24 h incubation with culture supernatants of species grown without selenium dioxide.


The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans.

Kheradmand E, Rafii F, Yazdi MH, Sepahi AA, Shahverdi AR, Oveisi MR - Daru (2014)

Viability of C. albicans after incubation in culture supernatants of L. johnsonii or L. plantarum, grown with or without selenium dioxide.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4060857&req=5

Figure 3: Viability of C. albicans after incubation in culture supernatants of L. johnsonii or L. plantarum, grown with or without selenium dioxide.
Mentions: The anti-Candida effects of the supernatants from L. plantarum and L. johnsonii, grown with and without selenium dioxide, were compared by measuring the zone of inhibition formed around each of the samples applied to the plates in the hole-plate diffusion method (Table 1). Negligible inhibition zones were observed on the plates containing culture supernatants of either of the species grown without selenium dioxide or sterile MRS broth supplemented with selenium dioxide (200 mg/l). Culture supernatants from L. plantarum and L. johnsonii grown with selenium dioxide for 48, 72, and 96 h showed potent anti-Candida activity and inhibited growth. The maximum antifungal activity was observed in 72 h cultures (Table 1). The time-kill assay, measuring the effect of culture supernatants of each strain, grown with or without selenium dioxide, on the viability of C. albicans, confirmed the increase in the antifungal activity of the strains grown with selenium dioxide (Figure 3). Whereas incubation of C. albicans with the supernatants of all cultures decreased viability, the number of surviving C. albicans cells substantially decreased when incubated with the supernatants of cultures grown for 72 h with selenium dioxide. The difference in the effect, which was observed even after 0.5 h incubation, increased with time. No viable C. albicans was present after 4 h incubation with culture supernatants of either species grown with selenium dioxide, but some viable C. albicans cells were present even after 24 h incubation with culture supernatants of species grown without selenium dioxide.

Bottom Line: The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles.Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. shahverd@tums.ac.ir.

ABSTRACT

Background: Lactic acid bacteria are considered important probiotics for prevention of some infections. The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.

Methods: Lactobacillus plantarum and L. johnsonii cells, grown in the presence and absence of selenium dioxide, and their cell-free spent culture media were tested for antifungal activity against C. albicans ATCC 14053 by a hole-plate diffusion method and a time-kill assay.

Results: Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles. The cell-free spent culture media, from both Lactobacillus species that had been grown with selenium dioxide for 48 h, showed enhanced antifungal activity against C. albicans. Enhanced antifungal activity of cell biomass against C. albicans was also observed in cultures grown with selenium dioxide.

Conclusions: Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

Show MeSH
Related in: MedlinePlus