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The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans.

Kheradmand E, Rafii F, Yazdi MH, Sepahi AA, Shahverdi AR, Oveisi MR - Daru (2014)

Bottom Line: The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles.Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. shahverd@tums.ac.ir.

ABSTRACT

Background: Lactic acid bacteria are considered important probiotics for prevention of some infections. The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.

Methods: Lactobacillus plantarum and L. johnsonii cells, grown in the presence and absence of selenium dioxide, and their cell-free spent culture media were tested for antifungal activity against C. albicans ATCC 14053 by a hole-plate diffusion method and a time-kill assay.

Results: Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles. The cell-free spent culture media, from both Lactobacillus species that had been grown with selenium dioxide for 48 h, showed enhanced antifungal activity against C. albicans. Enhanced antifungal activity of cell biomass against C. albicans was also observed in cultures grown with selenium dioxide.

Conclusions: Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

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Reduction of selenium dioxide concentration in cultures of L. johnsonii or L. plantarum, as determined by a spectrophotometric method.
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Figure 1: Reduction of selenium dioxide concentration in cultures of L. johnsonii or L. plantarum, as determined by a spectrophotometric method.

Mentions: After 24 h incubation with both L. plantarum and L. johnsonii, the concentrations of selenium in the supernatants were considerably reduced. Figure 1 shows the concentration of selenium remaining in the culture medium taken every 24 h. The amount of Se remaining in the supernatants of Lactobacillus cultures after 72 h was approximately 3 mg/l, indicating that 98.5% of the selenium ions were reduced in each of the Lactobacillus cultures (Figure 1). No appreciable amount of Se was present in the culture supernatant of any of the strains after 96 h of incubation. The selenium-enriched L. plantarum cells were selected for a TEM experiment (Figure 2A). Spherical SeNPs of various sizes had formed inside the L. plantarum cells (Figure 2B). Figure 2C shows a size histogram of the SeNPs inside the cells; the particle sizes ranged from 25 to 250 nm. Furthermore, EDX microanalysis of the separated NPs exhibited Se absorption peaks consisting of SeLα, SeKα and SeKβ at 1.37, 11.22 and 12.49 keV, respectively (Figure 2D) and confirmed the presence of Se in the samples.


The antimicrobial effects of selenium nanoparticle-enriched probiotics and their fermented broth against Candida albicans.

Kheradmand E, Rafii F, Yazdi MH, Sepahi AA, Shahverdi AR, Oveisi MR - Daru (2014)

Reduction of selenium dioxide concentration in cultures of L. johnsonii or L. plantarum, as determined by a spectrophotometric method.
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4060857&req=5

Figure 1: Reduction of selenium dioxide concentration in cultures of L. johnsonii or L. plantarum, as determined by a spectrophotometric method.
Mentions: After 24 h incubation with both L. plantarum and L. johnsonii, the concentrations of selenium in the supernatants were considerably reduced. Figure 1 shows the concentration of selenium remaining in the culture medium taken every 24 h. The amount of Se remaining in the supernatants of Lactobacillus cultures after 72 h was approximately 3 mg/l, indicating that 98.5% of the selenium ions were reduced in each of the Lactobacillus cultures (Figure 1). No appreciable amount of Se was present in the culture supernatant of any of the strains after 96 h of incubation. The selenium-enriched L. plantarum cells were selected for a TEM experiment (Figure 2A). Spherical SeNPs of various sizes had formed inside the L. plantarum cells (Figure 2B). Figure 2C shows a size histogram of the SeNPs inside the cells; the particle sizes ranged from 25 to 250 nm. Furthermore, EDX microanalysis of the separated NPs exhibited Se absorption peaks consisting of SeLα, SeKα and SeKβ at 1.37, 11.22 and 12.49 keV, respectively (Figure 2D) and confirmed the presence of Se in the samples.

Bottom Line: The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles.Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

View Article: PubMed Central - HTML - PubMed

Affiliation: Department of Pharmaceutical Biotechnology and Biotechnology Research Center, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran. shahverd@tums.ac.ir.

ABSTRACT

Background: Lactic acid bacteria are considered important probiotics for prevention of some infections. The aim of this work was to investigate the effect of selenium dioxide on the antifungal activity of Lactobacillus plantarum and L. johnsonii against Candida albicans.

Methods: Lactobacillus plantarum and L. johnsonii cells, grown in the presence and absence of selenium dioxide, and their cell-free spent culture media were tested for antifungal activity against C. albicans ATCC 14053 by a hole-plate diffusion method and a time-kill assay.

Results: Both L. plantarum and L. johnsonii reduced selenium dioxide to cell-associated elemental selenium nanoparticles. The cell-free spent culture media, from both Lactobacillus species that had been grown with selenium dioxide for 48 h, showed enhanced antifungal activity against C. albicans. Enhanced antifungal activity of cell biomass against C. albicans was also observed in cultures grown with selenium dioxide.

Conclusions: Selenium dioxide-treated Lactobacillus spp. or their cell-free spent broth inhibited the growth of C. albicans and should be investigated for possible use in anti-Candida probiotic formulations in future.

Show MeSH
Related in: MedlinePlus