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Exacerbated skeletal muscle inflammation and calcification in the acute phase of infection by Mexican Trypanosoma cruzi DTUI strain.

Vizcaíno-Castillo A, Jiménez-Marín A, Espinoza B - Biomed Res Int (2014)

Bottom Line: A murine model was used to study the histopathological aspects and cytokine expression levels in skeletal muscle provoked by the infection with Mexican TcI strains.In the acute phase of infection, a high parasitic load, both in blood and skeletal muscle, was detected.Interestingly, extensive calcification areas were observed in the skeletal muscle surrounded by inflammatory infiltrates.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.

ABSTRACT
A murine model was used to study the histopathological aspects and cytokine expression levels in skeletal muscle provoked by the infection with Mexican TcI strains. BALB/c mice were inoculated with the virulent Querétaro strain and the nonvirulent Ninoa strain. Parasite numbers were counted in blood and skeletal muscle at different times post-infection, and real time-PCR expression levels of the cytokines IL-12, IL-4, IL-10, IFN- γ , and TNF- α were evaluated. In the acute phase of infection, a high parasitic load, both in blood and skeletal muscle, was detected. The histopathological analyses showed an exacerbated inflammation and granulomatous-like infiltrate with the Querétaro strain. Interestingly, extensive calcification areas were observed in the skeletal muscle surrounded by inflammatory infiltrates. TNF- α and IL-10 expression exhibited a significant increase at the peak of infection. In summary, Querétaro strain, a Mexican TcI strain, is virulent enough to induce high inflammation and calcification in skeletal muscle of the hind limbs, which could be related to high expression levels of TNF- α .

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Macroscopic characteristics of a mouse infected with 10 000 trypomastigotes of Querétaro strain ((a), (c)) and a control mouse ((b), (d)). The Querétaro strain infection provokes back bristling hair and loss of mobility of their posterior extremities ((a); arrow). After sacrifice and removal of the skin, the skeletal muscle of mouse infected with Querétaro strain showed pale areas on the tissue ((c); arrowheads).
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fig1: Macroscopic characteristics of a mouse infected with 10 000 trypomastigotes of Querétaro strain ((a), (c)) and a control mouse ((b), (d)). The Querétaro strain infection provokes back bristling hair and loss of mobility of their posterior extremities ((a); arrow). After sacrifice and removal of the skin, the skeletal muscle of mouse infected with Querétaro strain showed pale areas on the tissue ((c); arrowheads).

Mentions: The animals infected with the Querétaro strain showed back bristling hair, a continuous tremor in their whole body, and loss of mobility of their rear extremities by days 13–15 pi (Figure 1(a)). These manifestations were coincident with the increasing in the parasitic load (Figure 2(a)), but they recovered some mobility of the hind legs by 90 days pi. On the contrary, neither the mice infected with Ninoa strain (not shown) nor the uninfected control mice (Figure 1(b)) showed these characteristics.


Exacerbated skeletal muscle inflammation and calcification in the acute phase of infection by Mexican Trypanosoma cruzi DTUI strain.

Vizcaíno-Castillo A, Jiménez-Marín A, Espinoza B - Biomed Res Int (2014)

Macroscopic characteristics of a mouse infected with 10 000 trypomastigotes of Querétaro strain ((a), (c)) and a control mouse ((b), (d)). The Querétaro strain infection provokes back bristling hair and loss of mobility of their posterior extremities ((a); arrow). After sacrifice and removal of the skin, the skeletal muscle of mouse infected with Querétaro strain showed pale areas on the tissue ((c); arrowheads).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060783&req=5

fig1: Macroscopic characteristics of a mouse infected with 10 000 trypomastigotes of Querétaro strain ((a), (c)) and a control mouse ((b), (d)). The Querétaro strain infection provokes back bristling hair and loss of mobility of their posterior extremities ((a); arrow). After sacrifice and removal of the skin, the skeletal muscle of mouse infected with Querétaro strain showed pale areas on the tissue ((c); arrowheads).
Mentions: The animals infected with the Querétaro strain showed back bristling hair, a continuous tremor in their whole body, and loss of mobility of their rear extremities by days 13–15 pi (Figure 1(a)). These manifestations were coincident with the increasing in the parasitic load (Figure 2(a)), but they recovered some mobility of the hind legs by 90 days pi. On the contrary, neither the mice infected with Ninoa strain (not shown) nor the uninfected control mice (Figure 1(b)) showed these characteristics.

Bottom Line: A murine model was used to study the histopathological aspects and cytokine expression levels in skeletal muscle provoked by the infection with Mexican TcI strains.In the acute phase of infection, a high parasitic load, both in blood and skeletal muscle, was detected.Interestingly, extensive calcification areas were observed in the skeletal muscle surrounded by inflammatory infiltrates.

View Article: PubMed Central - PubMed

Affiliation: Departamento de Inmunología, Instituto de Investigaciones Biomédicas, Universidad Nacional Autónoma de México, 04510 Mexico City, DF, Mexico.

ABSTRACT
A murine model was used to study the histopathological aspects and cytokine expression levels in skeletal muscle provoked by the infection with Mexican TcI strains. BALB/c mice were inoculated with the virulent Querétaro strain and the nonvirulent Ninoa strain. Parasite numbers were counted in blood and skeletal muscle at different times post-infection, and real time-PCR expression levels of the cytokines IL-12, IL-4, IL-10, IFN- γ , and TNF- α were evaluated. In the acute phase of infection, a high parasitic load, both in blood and skeletal muscle, was detected. The histopathological analyses showed an exacerbated inflammation and granulomatous-like infiltrate with the Querétaro strain. Interestingly, extensive calcification areas were observed in the skeletal muscle surrounded by inflammatory infiltrates. TNF- α and IL-10 expression exhibited a significant increase at the peak of infection. In summary, Querétaro strain, a Mexican TcI strain, is virulent enough to induce high inflammation and calcification in skeletal muscle of the hind limbs, which could be related to high expression levels of TNF- α .

Show MeSH
Related in: MedlinePlus