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Antioxidant and prophylactic effects of Delonix elata L., stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats.

Krishnappa P, Venkatarangaiah K - Biomed Res Int (2014)

Bottom Line: Bioassay-guided fractionation of DSE has resulted in the isolation and characterization of quercetin.DSE and quercetin have shown significant prophylactic effects by restoring the liver function markers (AST, ALT, ALP, serum bilirubin, and total protein) and antioxidant enzymes (SOD, CAT, GPx, and GST).These results were proved to be hepatoprotective at par with silymarin and well supported by the histological observations of liver sections with distinct hepatic cells, and mild degree of fatty change and necrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Post Graduate Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta, Karnataka 577 451, India.

ABSTRACT
Delonix elata L. (Ceasalpinaceae), is widely used by the traditional medical practitioners of Karnataka, India, to cure jaundice, and bronchial and rheumatic problems. The objective of this study was to screen the in vitro antioxidant and hepatoprotective activity of the stem bark extracts against CCl4-induced liver damage in rats. Among different stem bark extracts tested, the ethanol extract (DSE) has shown significant in vitro antioxidant property in radicals scavenging, metal chelating, and lipid peroxidation inhibition assays. HPLC analysis of the DSE revealed the presence of known antioxidant molecules, namely, gallic acid, ellagic acid, coumaric acid, quercetin, and rutin. Bioassay-guided fractionation of DSE has resulted in the isolation and characterization of quercetin. DSE and quercetin have shown significant prophylactic effects by restoring the liver function markers (AST, ALT, ALP, serum bilirubin, and total protein) and antioxidant enzymes (SOD, CAT, GPx, and GST). These results were proved to be hepatoprotective at par with silymarin and well supported by the histological observations of liver sections with distinct hepatic cells, and mild degree of fatty change and necrosis. The results indicated that the DSE and quercetin were significant for prophylactic activity against CCl4-induced liver damage in rats. This activity could be attributed to the antioxidant constituents in the DSE and hence justified the ethnomedicinal claims.

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(a) HPLC chromatogram of DSC for phenolic acids (1-gallic acid; 2-coumaric acid; UK-unknown); (b) HPLC chromatogram of DSE for phenolic acids (1-gallic acid; 2-coumaric acid; 3-ellagic acid; UK-unknown); (c) HPLC chromatogram of DSC for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown); (d) HPLC chromatogram of DSE for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown).
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fig1: (a) HPLC chromatogram of DSC for phenolic acids (1-gallic acid; 2-coumaric acid; UK-unknown); (b) HPLC chromatogram of DSE for phenolic acids (1-gallic acid; 2-coumaric acid; 3-ellagic acid; UK-unknown); (c) HPLC chromatogram of DSC for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown); (d) HPLC chromatogram of DSE for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown).

Mentions: The DSC and DSE were subjected to HPLC analysis for the characterization of phenolic acids and flavonoids (Table 2). The HPLC analysis of DSC revealed the presence of gallic acid, coumaric acid, and two unknown phenol compounds with retention time of 1.99, 3.18, 1.09, and 1.56 min (Figure 1(a)). The DSE contains gallic acid, coumaric acid, ellagic acid, and two unknowns with retention time of 1.89, 3.2, 3.97, 1.573, and 5.86 min, respectively (Figure 1(b)). Similarly, the HPLC-UV spectral peaks of DSC (Figure 1(c)) and DSE (Figure 1(d)) at 350 nm with the analysis of retention time of standard flavonoids showed the presence of flavonoid compounds, rutin, quercetin, and myricetin.


Antioxidant and prophylactic effects of Delonix elata L., stem bark extracts, and flavonoid isolated quercetin against carbon tetrachloride-induced hepatotoxicity in rats.

Krishnappa P, Venkatarangaiah K - Biomed Res Int (2014)

(a) HPLC chromatogram of DSC for phenolic acids (1-gallic acid; 2-coumaric acid; UK-unknown); (b) HPLC chromatogram of DSE for phenolic acids (1-gallic acid; 2-coumaric acid; 3-ellagic acid; UK-unknown); (c) HPLC chromatogram of DSC for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown); (d) HPLC chromatogram of DSE for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060769&req=5

fig1: (a) HPLC chromatogram of DSC for phenolic acids (1-gallic acid; 2-coumaric acid; UK-unknown); (b) HPLC chromatogram of DSE for phenolic acids (1-gallic acid; 2-coumaric acid; 3-ellagic acid; UK-unknown); (c) HPLC chromatogram of DSC for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown); (d) HPLC chromatogram of DSE for flavonoids (1-rutin; 2-quercetin; 3-myricetin; UK-unknown).
Mentions: The DSC and DSE were subjected to HPLC analysis for the characterization of phenolic acids and flavonoids (Table 2). The HPLC analysis of DSC revealed the presence of gallic acid, coumaric acid, and two unknown phenol compounds with retention time of 1.99, 3.18, 1.09, and 1.56 min (Figure 1(a)). The DSE contains gallic acid, coumaric acid, ellagic acid, and two unknowns with retention time of 1.89, 3.2, 3.97, 1.573, and 5.86 min, respectively (Figure 1(b)). Similarly, the HPLC-UV spectral peaks of DSC (Figure 1(c)) and DSE (Figure 1(d)) at 350 nm with the analysis of retention time of standard flavonoids showed the presence of flavonoid compounds, rutin, quercetin, and myricetin.

Bottom Line: Bioassay-guided fractionation of DSE has resulted in the isolation and characterization of quercetin.DSE and quercetin have shown significant prophylactic effects by restoring the liver function markers (AST, ALT, ALP, serum bilirubin, and total protein) and antioxidant enzymes (SOD, CAT, GPx, and GST).These results were proved to be hepatoprotective at par with silymarin and well supported by the histological observations of liver sections with distinct hepatic cells, and mild degree of fatty change and necrosis.

View Article: PubMed Central - PubMed

Affiliation: Department of Post Graduate Studies and Research in Biotechnology, Kuvempu University, Shankaraghatta, Karnataka 577 451, India.

ABSTRACT
Delonix elata L. (Ceasalpinaceae), is widely used by the traditional medical practitioners of Karnataka, India, to cure jaundice, and bronchial and rheumatic problems. The objective of this study was to screen the in vitro antioxidant and hepatoprotective activity of the stem bark extracts against CCl4-induced liver damage in rats. Among different stem bark extracts tested, the ethanol extract (DSE) has shown significant in vitro antioxidant property in radicals scavenging, metal chelating, and lipid peroxidation inhibition assays. HPLC analysis of the DSE revealed the presence of known antioxidant molecules, namely, gallic acid, ellagic acid, coumaric acid, quercetin, and rutin. Bioassay-guided fractionation of DSE has resulted in the isolation and characterization of quercetin. DSE and quercetin have shown significant prophylactic effects by restoring the liver function markers (AST, ALT, ALP, serum bilirubin, and total protein) and antioxidant enzymes (SOD, CAT, GPx, and GST). These results were proved to be hepatoprotective at par with silymarin and well supported by the histological observations of liver sections with distinct hepatic cells, and mild degree of fatty change and necrosis. The results indicated that the DSE and quercetin were significant for prophylactic activity against CCl4-induced liver damage in rats. This activity could be attributed to the antioxidant constituents in the DSE and hence justified the ethnomedicinal claims.

Show MeSH
Related in: MedlinePlus