Limits...
Simultaneous heterotrophic nitrification and aerobic denitrification by Chryseobacterium sp. R31 isolated from abattoir wastewater.

Kundu P, Pramanik A, Dasgupta A, Mukherjee S, Mukherjee J - Biomed Res Int (2014)

Bottom Line: From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a starting NH4 (+)-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact.Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative of Chryseobacterium haifense.This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genus Chryseobacterium and the associated kinetic coefficients.

View Article: PubMed Central - PubMed

Affiliation: Department of Civil Engineering, Jadavpur University, Kolkata 700 032, India.

ABSTRACT
A heterotrophic carbon utilizing microbe (R31) capable of simultaneous nitrification and denitrification (SND) was isolated from wastewater of an Indian slaughterhouse. From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a starting NH4 (+)-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact. The concentrations of the intermediates hydroxylamine, nitrite, and nitrate were low, thus ensuring nitrogen removal. Aerobic denitrification occurring during ammonium removal by R31 was confirmed by utilization of both nitrate and nitrite as nitrogen substrates. Glucose and succinate were superior while acetate and citrate were poor substrates for nitrogen removal. Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative of Chryseobacterium haifense. The NH4 (+)-N utilization rate and growth of strain R31 were found to be higher at C/N = 10 in comparison to those achieved with C/N ratios of 5 and 20. Monod kinetic coefficients, half saturation concentration (K s ), maximum rate of substrate utilization (k), yield coefficient, (Y) and endogenous decay coefficient (K d ) indicated potential application of R31 in large-scale SND process. This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genus Chryseobacterium and the associated kinetic coefficients.

Show MeSH

Related in: MedlinePlus

Time profile of ammonium oxidation by Chryseobacterium sp. R31. Ammonium nitrogen (filled diamonds), nitrite nitrogen (filled squares), nitrate nitrogen (filled triangles), and hydroxylamine (filled circle). Error bars represent one SD (n = 9).
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4060765&req=5

fig3: Time profile of ammonium oxidation by Chryseobacterium sp. R31. Ammonium nitrogen (filled diamonds), nitrite nitrogen (filled squares), nitrate nitrogen (filled triangles), and hydroxylamine (filled circle). Error bars represent one SD (n = 9).

Mentions: The profile of ammoniacal nitrogen utilization by isolate R31 with relation to time is also shown in Figure 3. After a contact period of 48 h, 95.87% of NH4+-N was consumed demonstrating the heterotrophic nitrification ability of the isolate. Figure 3 further depicts that the initial ammonia utilization rate was high and maximum stabilization of ammonium in solution was attained within 32 h, after which the utilization declined and ammonium was removed marginally. Exhaustion of ammonium led to reduced availability of the nitrogen substrate to the microorganism. Figure 3 also represents the corresponding nitrite nitrogen (NO2−-N) and nitrate nitrogen (NO3−-N) concentrations with respect to time. This figure shows that ammonium was substantially converted to nitrite and, after a reaction period of 24 h, the nitrite level in the reactor was maximum corresponding to 71.99% ammonium utilization. Nitrate concentration initially increased with time confirming heterotrophic nitrification by strain R31. Nitrate concentration reached its peak value at 32 h when 87.79% of ammonium was consumed. The decrease in nitrate concentration after reaching a peak value ascertained the denitrification process. Nitrate was utilized by the isolate after ammonium was exhausted. Hydroxylamine was detected in reduced amounts possibly due to the unstable nature of hydroxylamine and rapid transformation to the next intermediate, nitrite [14]. Simultaneous utilization of organic matter and ammonia degradation indicated R31 to be competent of heterotrophic nitrification as well as aerobic denitrification. Hydroxylamine, nitrite, and nitrate accumulation occurred with the decrease of ammonia nitrogen. The concentrations of the three intermediates were low, thus ensuring nitrogen removal. The profile of hydroxylamine, nitrite, and nitrate formation was similar to that observed during SND processes occurring in Agrobacterium sp. [9] as well as Klebsiella pneumonia [16]. The amount of ammonium removed (95.87% in 48 h) was higher than that striped off by Bacillus subtilis (58.4 ± 4.3% in 60 h) [13], Bacillus methylotrophicus (51.03% in 54 h) [14], and Marinobacter sp. F6 (48.62%) [7]. The ammonium utilization rate (1.15 mg/L/h) was similar to that obtained for Pseudomonas alcaligenes sp. AS-1 (1.15 mg/L/h) [10] and higher than the corresponding value of Bacillus sp. LY (0.43 mg/L/h) [10].


Simultaneous heterotrophic nitrification and aerobic denitrification by Chryseobacterium sp. R31 isolated from abattoir wastewater.

Kundu P, Pramanik A, Dasgupta A, Mukherjee S, Mukherjee J - Biomed Res Int (2014)

Time profile of ammonium oxidation by Chryseobacterium sp. R31. Ammonium nitrogen (filled diamonds), nitrite nitrogen (filled squares), nitrate nitrogen (filled triangles), and hydroxylamine (filled circle). Error bars represent one SD (n = 9).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060765&req=5

fig3: Time profile of ammonium oxidation by Chryseobacterium sp. R31. Ammonium nitrogen (filled diamonds), nitrite nitrogen (filled squares), nitrate nitrogen (filled triangles), and hydroxylamine (filled circle). Error bars represent one SD (n = 9).
Mentions: The profile of ammoniacal nitrogen utilization by isolate R31 with relation to time is also shown in Figure 3. After a contact period of 48 h, 95.87% of NH4+-N was consumed demonstrating the heterotrophic nitrification ability of the isolate. Figure 3 further depicts that the initial ammonia utilization rate was high and maximum stabilization of ammonium in solution was attained within 32 h, after which the utilization declined and ammonium was removed marginally. Exhaustion of ammonium led to reduced availability of the nitrogen substrate to the microorganism. Figure 3 also represents the corresponding nitrite nitrogen (NO2−-N) and nitrate nitrogen (NO3−-N) concentrations with respect to time. This figure shows that ammonium was substantially converted to nitrite and, after a reaction period of 24 h, the nitrite level in the reactor was maximum corresponding to 71.99% ammonium utilization. Nitrate concentration initially increased with time confirming heterotrophic nitrification by strain R31. Nitrate concentration reached its peak value at 32 h when 87.79% of ammonium was consumed. The decrease in nitrate concentration after reaching a peak value ascertained the denitrification process. Nitrate was utilized by the isolate after ammonium was exhausted. Hydroxylamine was detected in reduced amounts possibly due to the unstable nature of hydroxylamine and rapid transformation to the next intermediate, nitrite [14]. Simultaneous utilization of organic matter and ammonia degradation indicated R31 to be competent of heterotrophic nitrification as well as aerobic denitrification. Hydroxylamine, nitrite, and nitrate accumulation occurred with the decrease of ammonia nitrogen. The concentrations of the three intermediates were low, thus ensuring nitrogen removal. The profile of hydroxylamine, nitrite, and nitrate formation was similar to that observed during SND processes occurring in Agrobacterium sp. [9] as well as Klebsiella pneumonia [16]. The amount of ammonium removed (95.87% in 48 h) was higher than that striped off by Bacillus subtilis (58.4 ± 4.3% in 60 h) [13], Bacillus methylotrophicus (51.03% in 54 h) [14], and Marinobacter sp. F6 (48.62%) [7]. The ammonium utilization rate (1.15 mg/L/h) was similar to that obtained for Pseudomonas alcaligenes sp. AS-1 (1.15 mg/L/h) [10] and higher than the corresponding value of Bacillus sp. LY (0.43 mg/L/h) [10].

Bottom Line: From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a starting NH4 (+)-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact.Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative of Chryseobacterium haifense.This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genus Chryseobacterium and the associated kinetic coefficients.

View Article: PubMed Central - PubMed

Affiliation: Department of Civil Engineering, Jadavpur University, Kolkata 700 032, India.

ABSTRACT
A heterotrophic carbon utilizing microbe (R31) capable of simultaneous nitrification and denitrification (SND) was isolated from wastewater of an Indian slaughterhouse. From an initial COD value of 583.0 mg/L, 95.54% was removed whilst, from a starting NH4 (+)-N concentration of 55.7 mg/L, 95.87% was removed after 48 h contact. The concentrations of the intermediates hydroxylamine, nitrite, and nitrate were low, thus ensuring nitrogen removal. Aerobic denitrification occurring during ammonium removal by R31 was confirmed by utilization of both nitrate and nitrite as nitrogen substrates. Glucose and succinate were superior while acetate and citrate were poor substrates for nitrogen removal. Molecular phylogenetic identification, supported by chemotaxonomic and physiological properties, assigned R31 as a close relative of Chryseobacterium haifense. The NH4 (+)-N utilization rate and growth of strain R31 were found to be higher at C/N = 10 in comparison to those achieved with C/N ratios of 5 and 20. Monod kinetic coefficients, half saturation concentration (K s ), maximum rate of substrate utilization (k), yield coefficient, (Y) and endogenous decay coefficient (K d ) indicated potential application of R31 in large-scale SND process. This is the first report on concomitant carbon oxidation, nitrification, and denitrification in the genus Chryseobacterium and the associated kinetic coefficients.

Show MeSH
Related in: MedlinePlus