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Morphological changes in the suprachiasmatic nucleus of aging female marmosets (Callithrix jacchus).

Engelberth RC, Silva KD, Azevedo CV, Gavioli EC, dos Santos JR, Soares JG, Nascimento Junior ES, Cavalcante JC, Costa MS, Cavalcante JS - Biomed Res Int (2014)

Bottom Line: The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression.Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR.A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurochemical Studies, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

ABSTRACT
The suprachiasmatic nuclei (SCN) are pointed to as the mammals central circadian pacemaker. Aged animals show internal time disruption possibly caused by morphological and neurochemical changes in SCN components. Some studies reported changes of neuronal cells and neuroglia in the SCN of rats and nonhuman primates during aging. The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression. Therefore, our aim was to perform a comparative study of the morphological aspects of SCN in adult and aged female marmoset. Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR. A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

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Effects of age on the number of neurons in the suprachiasmatic nucleus (SCN) of the marmoset Callithrix jacchus: (a) number of neurons for Nissl, (b) NeuN, and (c) CB-IR (Calbindin immunoreactivity). Mann-Whitney test revealed statistically significant difference between groups (Adult and Aged) for all stained. Data are expressed as median and interquartile intervals (q3–q1). *P < 0.05 compared to adult group.
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fig2: Effects of age on the number of neurons in the suprachiasmatic nucleus (SCN) of the marmoset Callithrix jacchus: (a) number of neurons for Nissl, (b) NeuN, and (c) CB-IR (Calbindin immunoreactivity). Mann-Whitney test revealed statistically significant difference between groups (Adult and Aged) for all stained. Data are expressed as median and interquartile intervals (q3–q1). *P < 0.05 compared to adult group.

Mentions: These qualitative decreases in the number of cells in SCN sections observed in photomicrographs (Figure 1(c′) versus Figure 1(c)) were quantitatively documented. Mann-Whitney test revealed a significant decrease in the number of cells in the SCN stained by Nissl technique in aged compared to adult marmosets (adult marmoset: 110.0 (133.37–87.81); aged marmoset: 51.8 (55.62–49.64); data are represented as median (interquartile range q3–q1); P = 0.0209; Figure 2(a)) and NeuN (adult marmoset: 92.8 (108.06–75.12); aged marmoset: 37.4 (42.68–32.5); data are represented as median (interquartile ranges q3–q1); P = 0.0294; Figure 2(b)).


Morphological changes in the suprachiasmatic nucleus of aging female marmosets (Callithrix jacchus).

Engelberth RC, Silva KD, Azevedo CV, Gavioli EC, dos Santos JR, Soares JG, Nascimento Junior ES, Cavalcante JC, Costa MS, Cavalcante JS - Biomed Res Int (2014)

Effects of age on the number of neurons in the suprachiasmatic nucleus (SCN) of the marmoset Callithrix jacchus: (a) number of neurons for Nissl, (b) NeuN, and (c) CB-IR (Calbindin immunoreactivity). Mann-Whitney test revealed statistically significant difference between groups (Adult and Aged) for all stained. Data are expressed as median and interquartile intervals (q3–q1). *P < 0.05 compared to adult group.
© Copyright Policy
Related In: Results  -  Collection

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Show All Figures
getmorefigures.php?uid=PMC4060761&req=5

fig2: Effects of age on the number of neurons in the suprachiasmatic nucleus (SCN) of the marmoset Callithrix jacchus: (a) number of neurons for Nissl, (b) NeuN, and (c) CB-IR (Calbindin immunoreactivity). Mann-Whitney test revealed statistically significant difference between groups (Adult and Aged) for all stained. Data are expressed as median and interquartile intervals (q3–q1). *P < 0.05 compared to adult group.
Mentions: These qualitative decreases in the number of cells in SCN sections observed in photomicrographs (Figure 1(c′) versus Figure 1(c)) were quantitatively documented. Mann-Whitney test revealed a significant decrease in the number of cells in the SCN stained by Nissl technique in aged compared to adult marmosets (adult marmoset: 110.0 (133.37–87.81); aged marmoset: 51.8 (55.62–49.64); data are represented as median (interquartile range q3–q1); P = 0.0209; Figure 2(a)) and NeuN (adult marmoset: 92.8 (108.06–75.12); aged marmoset: 37.4 (42.68–32.5); data are represented as median (interquartile ranges q3–q1); P = 0.0294; Figure 2(b)).

Bottom Line: The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression.Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR.A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurochemical Studies, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

ABSTRACT
The suprachiasmatic nuclei (SCN) are pointed to as the mammals central circadian pacemaker. Aged animals show internal time disruption possibly caused by morphological and neurochemical changes in SCN components. Some studies reported changes of neuronal cells and neuroglia in the SCN of rats and nonhuman primates during aging. The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression. Therefore, our aim was to perform a comparative study of the morphological aspects of SCN in adult and aged female marmoset. Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR. A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

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