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Morphological changes in the suprachiasmatic nucleus of aging female marmosets (Callithrix jacchus).

Engelberth RC, Silva KD, Azevedo CV, Gavioli EC, dos Santos JR, Soares JG, Nascimento Junior ES, Cavalcante JC, Costa MS, Cavalcante JS - Biomed Res Int (2014)

Bottom Line: The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression.Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR.A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurochemical Studies, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

ABSTRACT
The suprachiasmatic nuclei (SCN) are pointed to as the mammals central circadian pacemaker. Aged animals show internal time disruption possibly caused by morphological and neurochemical changes in SCN components. Some studies reported changes of neuronal cells and neuroglia in the SCN of rats and nonhuman primates during aging. The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression. Therefore, our aim was to perform a comparative study of the morphological aspects of SCN in adult and aged female marmoset. Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR. A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

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The suprachiasmatic nucleus. Digital images of coronal sections at hypothalamic level of the marmoset brain showing SCN at Nissl stained sections in adult (a) and aged (a′) animals; ((b) and (b′)) NeuN-immunostained; and ((c) and (c′)) CB-immunostained. Asterisk (*) shows degenerative areas and the rectangle in the figure represents the count area. CB: Calbindin; 3v: third ventricle; oc: optic chiasm. Scale bar: 50 μm.
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fig1: The suprachiasmatic nucleus. Digital images of coronal sections at hypothalamic level of the marmoset brain showing SCN at Nissl stained sections in adult (a) and aged (a′) animals; ((b) and (b′)) NeuN-immunostained; and ((c) and (c′)) CB-immunostained. Asterisk (*) shows degenerative areas and the rectangle in the figure represents the count area. CB: Calbindin; 3v: third ventricle; oc: optic chiasm. Scale bar: 50 μm.

Mentions: In SCN sections Nissl-stained (Figures 1(a) and 1(a′)), or marked to NeuN-IR (Figures 1(b) and 1(b′)) and CB-IR (Figures 1(c) and 1(c′)), a decrease in the number of cells was observed in aged marmosets when compared with adult animals. In aged animals, sections stained with Nissl (Figure 1(a′)) and NeuN (Figure 1(b′)) revealed many whitish areas throughout the SCN. However, these whitish areas were not observed in adult animals, which suggest the occurrence of neurodegeneration (Figures 1(a) and 1(b)). For immunostaining to CB, it is also possible to qualitatively observe a decrease in the number of cells available in the SCN of aged marmosets (Figure 1(c′)) compared to adult animals (Figure 1(c)).


Morphological changes in the suprachiasmatic nucleus of aging female marmosets (Callithrix jacchus).

Engelberth RC, Silva KD, Azevedo CV, Gavioli EC, dos Santos JR, Soares JG, Nascimento Junior ES, Cavalcante JC, Costa MS, Cavalcante JS - Biomed Res Int (2014)

The suprachiasmatic nucleus. Digital images of coronal sections at hypothalamic level of the marmoset brain showing SCN at Nissl stained sections in adult (a) and aged (a′) animals; ((b) and (b′)) NeuN-immunostained; and ((c) and (c′)) CB-immunostained. Asterisk (*) shows degenerative areas and the rectangle in the figure represents the count area. CB: Calbindin; 3v: third ventricle; oc: optic chiasm. Scale bar: 50 μm.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060761&req=5

fig1: The suprachiasmatic nucleus. Digital images of coronal sections at hypothalamic level of the marmoset brain showing SCN at Nissl stained sections in adult (a) and aged (a′) animals; ((b) and (b′)) NeuN-immunostained; and ((c) and (c′)) CB-immunostained. Asterisk (*) shows degenerative areas and the rectangle in the figure represents the count area. CB: Calbindin; 3v: third ventricle; oc: optic chiasm. Scale bar: 50 μm.
Mentions: In SCN sections Nissl-stained (Figures 1(a) and 1(a′)), or marked to NeuN-IR (Figures 1(b) and 1(b′)) and CB-IR (Figures 1(c) and 1(c′)), a decrease in the number of cells was observed in aged marmosets when compared with adult animals. In aged animals, sections stained with Nissl (Figure 1(a′)) and NeuN (Figure 1(b′)) revealed many whitish areas throughout the SCN. However, these whitish areas were not observed in adult animals, which suggest the occurrence of neurodegeneration (Figures 1(a) and 1(b)). For immunostaining to CB, it is also possible to qualitatively observe a decrease in the number of cells available in the SCN of aged marmosets (Figure 1(c′)) compared to adult animals (Figure 1(c)).

Bottom Line: The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression.Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR.A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of Neurochemical Studies, Physiology Department, Biosciences Center, Federal University of Rio Grande do Norte, Natal, RN, Brazil.

ABSTRACT
The suprachiasmatic nuclei (SCN) are pointed to as the mammals central circadian pacemaker. Aged animals show internal time disruption possibly caused by morphological and neurochemical changes in SCN components. Some studies reported changes of neuronal cells and neuroglia in the SCN of rats and nonhuman primates during aging. The effects of senescence on morphological aspects in SCN are important for understanding some alterations in biological rhythms expression. Therefore, our aim was to perform a comparative study of the morphological aspects of SCN in adult and aged female marmoset. Morphometric analysis of SCN was performed using Nissl staining, NeuN-IR, GFAP-IR, and CB-IR. A significant decrease in the SCN cells staining with Nissl, NeuN, and CB were observed in aged female marmosets compared to adults, while a significant increase in glial cells was found in aged marmosets, thus suggesting compensatory process due to neuronal loss evoked by aging.

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