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Comparative examination of the olive mill wastewater biodegradation process by various wood-rot macrofungi.

Koutrotsios G, Zervakis GI - Biomed Res Int (2014)

Bottom Line: Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization.Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities.Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

ABSTRACT
Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapalopilus croceus, Hericium erinaceus, Irpex lacteus, Phanerochaete chrysosporium, Pleurotus djamor, and P. pulmonarius. Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization. A. biennis was the best performing strain (it decreased phenolics by 92% and color by 64%) followed by P. djamor and I. lacteus. Increase of plant seeds germination was less pronounced evidencing that phenolics are only partly responsible for OMW's phytotoxicity. Laccase production was highly correlated with all three biodegradation parameters for H. croceus, Ph. chrysosporium, and Pleurotus spp., and so were manganese-independent and manganese dependent peroxidases for A. biennis and I. lacteus. Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities. Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

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Coefficient of determination (r2) values for OMW decolorization and total phenolics reduction versus the laccase activities presented by the eight selected macrofungi as calculated from the respective data obtained at five time points (T1 to T5) during their entire growth period.
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fig3: Coefficient of determination (r2) values for OMW decolorization and total phenolics reduction versus the laccase activities presented by the eight selected macrofungi as calculated from the respective data obtained at five time points (T1 to T5) during their entire growth period.

Mentions: It is particularly noteworthy that when the experimental data from all eight fungi were assembled and evaluated vis-à-vis their total phenol reduction, decolorization, plant seed germination increase, and enzymes activity (Table 5), then biomass and germination index showed statistically significant correlations in all comparisons (r2 = 0.55–0.88 and r2 = 0.51–0.88, resp.). Laccase production was significantly correlated with total phenolics reduction and decolorization (Figure 3), and so was decolorization with MnIP (r2 = 0.46). Last, MnP, and MnIP activities were also correlated (r2 = 0.55).


Comparative examination of the olive mill wastewater biodegradation process by various wood-rot macrofungi.

Koutrotsios G, Zervakis GI - Biomed Res Int (2014)

Coefficient of determination (r2) values for OMW decolorization and total phenolics reduction versus the laccase activities presented by the eight selected macrofungi as calculated from the respective data obtained at five time points (T1 to T5) during their entire growth period.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060750&req=5

fig3: Coefficient of determination (r2) values for OMW decolorization and total phenolics reduction versus the laccase activities presented by the eight selected macrofungi as calculated from the respective data obtained at five time points (T1 to T5) during their entire growth period.
Mentions: It is particularly noteworthy that when the experimental data from all eight fungi were assembled and evaluated vis-à-vis their total phenol reduction, decolorization, plant seed germination increase, and enzymes activity (Table 5), then biomass and germination index showed statistically significant correlations in all comparisons (r2 = 0.55–0.88 and r2 = 0.51–0.88, resp.). Laccase production was significantly correlated with total phenolics reduction and decolorization (Figure 3), and so was decolorization with MnIP (r2 = 0.46). Last, MnP, and MnIP activities were also correlated (r2 = 0.55).

Bottom Line: Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization.Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities.Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

ABSTRACT
Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapalopilus croceus, Hericium erinaceus, Irpex lacteus, Phanerochaete chrysosporium, Pleurotus djamor, and P. pulmonarius. Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization. A. biennis was the best performing strain (it decreased phenolics by 92% and color by 64%) followed by P. djamor and I. lacteus. Increase of plant seeds germination was less pronounced evidencing that phenolics are only partly responsible for OMW's phytotoxicity. Laccase production was highly correlated with all three biodegradation parameters for H. croceus, Ph. chrysosporium, and Pleurotus spp., and so were manganese-independent and manganese dependent peroxidases for A. biennis and I. lacteus. Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities. Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

Show MeSH
Related in: MedlinePlus