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Comparative examination of the olive mill wastewater biodegradation process by various wood-rot macrofungi.

Koutrotsios G, Zervakis GI - Biomed Res Int (2014)

Bottom Line: Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization.Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities.Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

ABSTRACT
Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapalopilus croceus, Hericium erinaceus, Irpex lacteus, Phanerochaete chrysosporium, Pleurotus djamor, and P. pulmonarius. Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization. A. biennis was the best performing strain (it decreased phenolics by 92% and color by 64%) followed by P. djamor and I. lacteus. Increase of plant seeds germination was less pronounced evidencing that phenolics are only partly responsible for OMW's phytotoxicity. Laccase production was highly correlated with all three biodegradation parameters for H. croceus, Ph. chrysosporium, and Pleurotus spp., and so were manganese-independent and manganese dependent peroxidases for A. biennis and I. lacteus. Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities. Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

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Mycelium linear growth rates (Kr, mm day−1) on PDA as measured at different temperatures (°C) for selected indicative cases of macrofungi examined (i.e., A. biennis ABL436, H. croceus HCC522, I. lacteus ILC238, Ph. chrysosporium PHL322, and P. djamor PDC855).
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fig1: Mycelium linear growth rates (Kr, mm day−1) on PDA as measured at different temperatures (°C) for selected indicative cases of macrofungi examined (i.e., A. biennis ABL436, H. croceus HCC522, I. lacteus ILC238, Ph. chrysosporium PHL322, and P. djamor PDC855).

Mentions: Data about the optimal conditions for the production of fungal biomass are available for a rather few species of macrofungi (i.e., mainly those exploited for edible mushroom production). The effect of temperature, in particular, is of fundamental importance in evaluating the biotechnological potential of such organisms [33, 34], and hence it was the first parameter which was assessed in the present study. The results of the comparative mycelium linear growth rate examination covering a wide temperature range (12–47°C) showed that most strains presented their optima at a narrow value area of 27–32°C (Table 2). Only Hypsizygus ulmarius, Omphalotus illudens, Pholiota nameko, and Pleurotus nebrodensis exhibited significantly higher growth rates at 22°C. On the other hand, Phanerochaete chrysosporium PHC571 was the only one presenting optimal growth at 37°C. It is noteworthy that almost all Pleurotus strains showed a growth (albeit limited) at 12°C; Pleurotus was the only genus among the 28 different genera evaluated demonstrating such a property. In contrast, Hapalopilus croceus and Phanerochaete chrysosporium were the only species showing hyphal development at the other temperature extreme (42°C). Furthermore, mycelium growth rates of almost all strains presented a gradual increase from lower temperatures to the optimal one and then a sharp decrease to the temperature of no growth; in fact, this effect was more pronounced for individuals demonstrating high growth values (Figure 1).


Comparative examination of the olive mill wastewater biodegradation process by various wood-rot macrofungi.

Koutrotsios G, Zervakis GI - Biomed Res Int (2014)

Mycelium linear growth rates (Kr, mm day−1) on PDA as measured at different temperatures (°C) for selected indicative cases of macrofungi examined (i.e., A. biennis ABL436, H. croceus HCC522, I. lacteus ILC238, Ph. chrysosporium PHL322, and P. djamor PDC855).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4060750&req=5

fig1: Mycelium linear growth rates (Kr, mm day−1) on PDA as measured at different temperatures (°C) for selected indicative cases of macrofungi examined (i.e., A. biennis ABL436, H. croceus HCC522, I. lacteus ILC238, Ph. chrysosporium PHL322, and P. djamor PDC855).
Mentions: Data about the optimal conditions for the production of fungal biomass are available for a rather few species of macrofungi (i.e., mainly those exploited for edible mushroom production). The effect of temperature, in particular, is of fundamental importance in evaluating the biotechnological potential of such organisms [33, 34], and hence it was the first parameter which was assessed in the present study. The results of the comparative mycelium linear growth rate examination covering a wide temperature range (12–47°C) showed that most strains presented their optima at a narrow value area of 27–32°C (Table 2). Only Hypsizygus ulmarius, Omphalotus illudens, Pholiota nameko, and Pleurotus nebrodensis exhibited significantly higher growth rates at 22°C. On the other hand, Phanerochaete chrysosporium PHC571 was the only one presenting optimal growth at 37°C. It is noteworthy that almost all Pleurotus strains showed a growth (albeit limited) at 12°C; Pleurotus was the only genus among the 28 different genera evaluated demonstrating such a property. In contrast, Hapalopilus croceus and Phanerochaete chrysosporium were the only species showing hyphal development at the other temperature extreme (42°C). Furthermore, mycelium growth rates of almost all strains presented a gradual increase from lower temperatures to the optimal one and then a sharp decrease to the temperature of no growth; in fact, this effect was more pronounced for individuals demonstrating high growth values (Figure 1).

Bottom Line: Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization.Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities.Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

View Article: PubMed Central - PubMed

Affiliation: Laboratory of General and Agricultural Microbiology, Agricultural University of Athens, Iera Odos 75, 11855 Athens, Greece.

ABSTRACT
Olive mill wastewater (OMW) constitutes a major cause of environmental pollution in olive-oil producing regions. Sixty wood-rot macrofungi assigned in 43 species were evaluated for their efficacy to colonize solidified OMW media at initially established optimal growth temperatures. Subsequently eight strains of the following species were qualified: Abortiporus biennis, Ganoderma carnosum, Hapalopilus croceus, Hericium erinaceus, Irpex lacteus, Phanerochaete chrysosporium, Pleurotus djamor, and P. pulmonarius. Fungal growth in OMW (25%v/v in water) resulted in marked reduction of total phenolic content, which was significantly correlated with the effluent's decolorization. A. biennis was the best performing strain (it decreased phenolics by 92% and color by 64%) followed by P. djamor and I. lacteus. Increase of plant seeds germination was less pronounced evidencing that phenolics are only partly responsible for OMW's phytotoxicity. Laccase production was highly correlated with all three biodegradation parameters for H. croceus, Ph. chrysosporium, and Pleurotus spp., and so were manganese-independent and manganese dependent peroxidases for A. biennis and I. lacteus. Monitoring of enzymes with respect to biomass production indicated that Pleurotus spp., H. croceus, and Ph. chrysosporium shared common patterns for all three activities. Moreover, generation of enzymes at the early biodegradation stages enhanced the efficiency of OMW treatment.

Show MeSH
Related in: MedlinePlus