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Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Escherichia coli in Italy.

Coppo E, Del Bono V, Ventura F, Camera M, Orengo G, Viscoli C, Marchese A - BMC Microbiol. (2014)

Bottom Line: Genetic features associated with blaNDM-4 gene were investigated.MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology Unit DISC, University of Genoa, IRCCS AOU San Martino-IST, Largo R, Benzi 10, 16132 Genoa, Italy. anna.marchese@unige.it.

ABSTRACT

Background: During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated.

Results: The isolates exhibited the same PFGE profile and a multidrug-resistant (MDR) phenotype to aminoglycosides, fluoroquinolones, and β-lactams. The strains produced the NDM-4 carbapenemase and the blaNDM-4 gene was part of the variable region of a class 1 integron. MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).

Conclusions: This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.

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Schematic representation of genetic structures surrounding blaNDM4 (A) and structure of class 1 integron (B).
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Figure 3: Schematic representation of genetic structures surrounding blaNDM4 (A) and structure of class 1 integron (B).

Mentions: In the index isolate, PCR and sequencing analysis detected the presence of blaNDM-4 and of the following acquired resistance genes: blaTEM-1, blaCTX-M-15,dfrA12, aac (3)-II, aadA2. No other carbapenemase genes (OXA-48 or VIM types) were identified in these isolate. The resistance determinants dfrA12 and aadA2 were carried on gene cassette inserted into a class 1 integron (Figure 3), resulting in a cassette array identical to that previously described in E.coli GUE-NDM isolate from India (accession number JQ364967).


Identification of a New Delhi metallo-β-lactamase-4 (NDM-4)-producing Escherichia coli in Italy.

Coppo E, Del Bono V, Ventura F, Camera M, Orengo G, Viscoli C, Marchese A - BMC Microbiol. (2014)

Schematic representation of genetic structures surrounding blaNDM4 (A) and structure of class 1 integron (B).
© Copyright Policy - open-access
Related In: Results  -  Collection

License 1 - License 2
Show All Figures
getmorefigures.php?uid=PMC4060580&req=5

Figure 3: Schematic representation of genetic structures surrounding blaNDM4 (A) and structure of class 1 integron (B).
Mentions: In the index isolate, PCR and sequencing analysis detected the presence of blaNDM-4 and of the following acquired resistance genes: blaTEM-1, blaCTX-M-15,dfrA12, aac (3)-II, aadA2. No other carbapenemase genes (OXA-48 or VIM types) were identified in these isolate. The resistance determinants dfrA12 and aadA2 were carried on gene cassette inserted into a class 1 integron (Figure 3), resulting in a cassette array identical to that previously described in E.coli GUE-NDM isolate from India (accession number JQ364967).

Bottom Line: Genetic features associated with blaNDM-4 gene were investigated.MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.

View Article: PubMed Central - HTML - PubMed

Affiliation: Microbiology Unit DISC, University of Genoa, IRCCS AOU San Martino-IST, Largo R, Benzi 10, 16132 Genoa, Italy. anna.marchese@unige.it.

ABSTRACT

Background: During June-July 2012, six imipenem-resistant Escherichia coli isolates were isolated from two patients hospitalized in a ward of one large tertiary-care hospital in Genoa, Italy. Genetic features associated with blaNDM-4 gene were investigated.

Results: The isolates exhibited the same PFGE profile and a multidrug-resistant (MDR) phenotype to aminoglycosides, fluoroquinolones, and β-lactams. The strains produced the NDM-4 carbapenemase and the blaNDM-4 gene was part of the variable region of a class 1 integron. MLST analysis revealed that all isolates belonged to sequence type 405 (ST405).

Conclusions: This is the first report on the emergence of an MDR strain of E.coli producing the NDM-4 MBL in Italy.

Show MeSH
Related in: MedlinePlus