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Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Study of PLGA scaffold degradation in PBS at 37°C up to 28 days. (A–E) SEM micrographs of PLGA group weekly observed. Degradation behavior of PLGAH001FN samples up to (F) 7, (G) 14, and (H) 21 days. (J i–iii) Details of fiber agglutination (black arrows), appeared pores (white arrows), and pore dimensions of PLGAH001FN samples up to 21 days of PBS immersion. Scale bar: (A–E) 100 μm; (F–H) 1 mm; (J i–iii) 20 μm. (I) Changes of remaining weight and (K) variations of pH values during in vitro degradation up to 28 days.
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f5: Study of PLGA scaffold degradation in PBS at 37°C up to 28 days. (A–E) SEM micrographs of PLGA group weekly observed. Degradation behavior of PLGAH001FN samples up to (F) 7, (G) 14, and (H) 21 days. (J i–iii) Details of fiber agglutination (black arrows), appeared pores (white arrows), and pore dimensions of PLGAH001FN samples up to 21 days of PBS immersion. Scale bar: (A–E) 100 μm; (F–H) 1 mm; (J i–iii) 20 μm. (I) Changes of remaining weight and (K) variations of pH values during in vitro degradation up to 28 days.

Mentions: Loss of fiber alignment, fiber scissions, and loss of porosity during the hydrolysis degradation process were observed during in vitro degradation assay up to 28 days (Fig. 5). PLGA scaffold groups suffered a loss of mass and strong structural modifications. The mass of untreated PLGA scaffolds was stable up to 28 days (Fig. 5I). In general, the profiles from treated PLGA scaffolds presented an increase in scaffold mass during the first week, followed by a decrease in mass behavior (Fig. 5I). No important mass variations in measurements were observed for PLGAH001 and PLGAH001FN samples. A weekly morphological evolution of untreated PLGA scaffolds is shown in Figure 5A–E. More hydrophilic PLGAH001FN samples have shown typically and more significant shrinkage behavior with loss of porosity during scaffold degradation (Fig. 5F–H). In detail, these hydrolyzed, coated fibers became collapsed (Fig. 5J-i, black arrows), with the presence of heterogeneous pores (from 497.1 nm to 3.279 μm in diameter) on the PLGA fiber surface up to 21 days (Fig. 5 J-ii, iii). The presence of pores was intensified during degradation assay progression (data not shown). From the pH value variation, the results showed no drastic changes in the pH values for any of the groups (Fig. 5K).


Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Study of PLGA scaffold degradation in PBS at 37°C up to 28 days. (A–E) SEM micrographs of PLGA group weekly observed. Degradation behavior of PLGAH001FN samples up to (F) 7, (G) 14, and (H) 21 days. (J i–iii) Details of fiber agglutination (black arrows), appeared pores (white arrows), and pore dimensions of PLGAH001FN samples up to 21 days of PBS immersion. Scale bar: (A–E) 100 μm; (F–H) 1 mm; (J i–iii) 20 μm. (I) Changes of remaining weight and (K) variations of pH values during in vitro degradation up to 28 days.
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Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4048976&req=5

f5: Study of PLGA scaffold degradation in PBS at 37°C up to 28 days. (A–E) SEM micrographs of PLGA group weekly observed. Degradation behavior of PLGAH001FN samples up to (F) 7, (G) 14, and (H) 21 days. (J i–iii) Details of fiber agglutination (black arrows), appeared pores (white arrows), and pore dimensions of PLGAH001FN samples up to 21 days of PBS immersion. Scale bar: (A–E) 100 μm; (F–H) 1 mm; (J i–iii) 20 μm. (I) Changes of remaining weight and (K) variations of pH values during in vitro degradation up to 28 days.
Mentions: Loss of fiber alignment, fiber scissions, and loss of porosity during the hydrolysis degradation process were observed during in vitro degradation assay up to 28 days (Fig. 5). PLGA scaffold groups suffered a loss of mass and strong structural modifications. The mass of untreated PLGA scaffolds was stable up to 28 days (Fig. 5I). In general, the profiles from treated PLGA scaffolds presented an increase in scaffold mass during the first week, followed by a decrease in mass behavior (Fig. 5I). No important mass variations in measurements were observed for PLGAH001 and PLGAH001FN samples. A weekly morphological evolution of untreated PLGA scaffolds is shown in Figure 5A–E. More hydrophilic PLGAH001FN samples have shown typically and more significant shrinkage behavior with loss of porosity during scaffold degradation (Fig. 5F–H). In detail, these hydrolyzed, coated fibers became collapsed (Fig. 5J-i, black arrows), with the presence of heterogeneous pores (from 497.1 nm to 3.279 μm in diameter) on the PLGA fiber surface up to 21 days (Fig. 5 J-ii, iii). The presence of pores was intensified during degradation assay progression (data not shown). From the pH value variation, the results showed no drastic changes in the pH values for any of the groups (Fig. 5K).

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus