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Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Mechanical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds: (A) Tensile stress (MPa) versus strain (%) curves and (B) tensile modulus (MPa). Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
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f4: Mechanical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds: (A) Tensile stress (MPa) versus strain (%) curves and (B) tensile modulus (MPa). Data are expressed as mean±SD. *Significant difference between groups (p<0.05).

Mentions: The results of the mechanical properties are summarized in Figure 4. Compared with untreated PLGA scaffolds, hydrolysis treatment caused a significant decrease in the tensile modulus from 75±14 MPa for PLGA group to 45±22 MPa for PLGAH01 and to 65±19 MPa for PLGAH001. The presence of FN coating was significant to decrease mechanical properties: to 13.2±7.8 MPa for PLGAH01FN and to 15.9±3.4 MPa for PLGAH001FN (Fig. 4).


Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Mechanical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds: (A) Tensile stress (MPa) versus strain (%) curves and (B) tensile modulus (MPa). Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4048976&req=5

f4: Mechanical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds: (A) Tensile stress (MPa) versus strain (%) curves and (B) tensile modulus (MPa). Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
Mentions: The results of the mechanical properties are summarized in Figure 4. Compared with untreated PLGA scaffolds, hydrolysis treatment caused a significant decrease in the tensile modulus from 75±14 MPa for PLGA group to 45±22 MPa for PLGAH01 and to 65±19 MPa for PLGAH001. The presence of FN coating was significant to decrease mechanical properties: to 13.2±7.8 MPa for PLGAH01FN and to 15.9±3.4 MPa for PLGAH001FN (Fig. 4).

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus