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Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus

Chemical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds by (A) ATR-FT-IR and (B) XPS spectra. Control groups: PLGA scaffold immersed in PBS (PLGA PBS) and in fibronectin solution (PLGA FN) for 24 h. ATR-FT-IR, attenuated total reflectance Fourier transform infrared; XPS, X-ray photoelectron spectroscopy.
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f3: Chemical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds by (A) ATR-FT-IR and (B) XPS spectra. Control groups: PLGA scaffold immersed in PBS (PLGA PBS) and in fibronectin solution (PLGA FN) for 24 h. ATR-FT-IR, attenuated total reflectance Fourier transform infrared; XPS, X-ray photoelectron spectroscopy.

Mentions: Hydrolysis may induce chemical modifications, and the preservation of PLGA chains was verified by ATR-FT-IR and XPS. Typical spectra of PLGA polymer-based material were found from ATR-FT-IR and XPS analysis for all analyzed groups (Fig. 3 A,B). By FT-IR, a strong ether carbonyl stretch (C=O) absorption band of the PLGA group was found at 1747 cm−1; ether group stretching (C–O–C) at 1083 cm−1 and methyl stretching (C–H) and (C–CH3) groups at 1450 and 1043 cm−1, respectively, were also found. These bands could also be observed in the all FT-IR spectra from the hydrolyzed, coated scaffolds without other significant changes. From XPS results, all spectra were similar between groups with C–C and C–H of C1s at 285 eV. The N1s pic corresponding to N–C organic bindings was detected at 399.7 eV. From both of the hydrolyzed, FN-coated samples' spectra, a pic at 401.7 eV was visualized and corresponds to N=O bindings. The presence of the P2p pic in phosphate form was detected at 133.5 eV, and it may be related to PBS. Similarly, the spectrum of PLGA PBS presented pics of Na and Cl elements, probably from the PBS. The elementary composition of PLGA chains was also evidenced by the similar O/C values (Table 3). Even if a lower O/C value visualized for PLGAH001FN groups was because of the lower presence of oxygen at the sample surface, higher values of carbon and nitrogen percentage were observed: 61.1% and 3.3%, respectively (Table 3).


Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Chemical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds by (A) ATR-FT-IR and (B) XPS spectra. Control groups: PLGA scaffold immersed in PBS (PLGA PBS) and in fibronectin solution (PLGA FN) for 24 h. ATR-FT-IR, attenuated total reflectance Fourier transform infrared; XPS, X-ray photoelectron spectroscopy.
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4048976&req=5

f3: Chemical characterization of untreated, hydrolyzed, and hydrolyzed-and-coated PLGA scaffolds by (A) ATR-FT-IR and (B) XPS spectra. Control groups: PLGA scaffold immersed in PBS (PLGA PBS) and in fibronectin solution (PLGA FN) for 24 h. ATR-FT-IR, attenuated total reflectance Fourier transform infrared; XPS, X-ray photoelectron spectroscopy.
Mentions: Hydrolysis may induce chemical modifications, and the preservation of PLGA chains was verified by ATR-FT-IR and XPS. Typical spectra of PLGA polymer-based material were found from ATR-FT-IR and XPS analysis for all analyzed groups (Fig. 3 A,B). By FT-IR, a strong ether carbonyl stretch (C=O) absorption band of the PLGA group was found at 1747 cm−1; ether group stretching (C–O–C) at 1083 cm−1 and methyl stretching (C–H) and (C–CH3) groups at 1450 and 1043 cm−1, respectively, were also found. These bands could also be observed in the all FT-IR spectra from the hydrolyzed, coated scaffolds without other significant changes. From XPS results, all spectra were similar between groups with C–C and C–H of C1s at 285 eV. The N1s pic corresponding to N–C organic bindings was detected at 399.7 eV. From both of the hydrolyzed, FN-coated samples' spectra, a pic at 401.7 eV was visualized and corresponds to N=O bindings. The presence of the P2p pic in phosphate form was detected at 133.5 eV, and it may be related to PBS. Similarly, the spectrum of PLGA PBS presented pics of Na and Cl elements, probably from the PBS. The elementary composition of PLGA chains was also evidenced by the similar O/C values (Table 3). Even if a lower O/C value visualized for PLGAH001FN groups was because of the lower presence of oxygen at the sample surface, higher values of carbon and nitrogen percentage were observed: 61.1% and 3.3%, respectively (Table 3).

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus