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Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

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Related in: MedlinePlus

(A–F) SEM micrographs, contact angle water drop images, and fiber orientation (arrows) of electrospun PLGA groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. SEM scale bar, 500 μm. (G) Fiber diameter and (H) scaffold porosity of untreated and treated PLGA fibers measured by image treatment. Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
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f2: (A–F) SEM micrographs, contact angle water drop images, and fiber orientation (arrows) of electrospun PLGA groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. SEM scale bar, 500 μm. (G) Fiber diameter and (H) scaffold porosity of untreated and treated PLGA fibers measured by image treatment. Data are expressed as mean±SD. *Significant difference between groups (p<0.05).

Mentions: Structural modifications led to loss of preferential fiber orientation (Fig. 2). Irregular alignment was evident after scaffold hydration, hydrolysis, and FN functionalization (Fig. 2B–F). For the PLGAH01 and PLGAH01FN, porosity results (Fig. 2H) indicated that the space between the fibers became significantly larger, thus generating overlapping sheets on heterogeneous regions of surface (Fig. 2C). Despite of collapsed morphology, no significant difference in fiber diameter was found between PLGAH01 groups and untreated fibers (Fig. 2G). PLGA H001FN showed fibers with a significantly larger diameter compared with both of PLGAH01 groups (Fig. 2G).


Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

(A–F) SEM micrographs, contact angle water drop images, and fiber orientation (arrows) of electrospun PLGA groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. SEM scale bar, 500 μm. (G) Fiber diameter and (H) scaffold porosity of untreated and treated PLGA fibers measured by image treatment. Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
© Copyright Policy
Related In: Results  -  Collection

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getmorefigures.php?uid=PMC4048976&req=5

f2: (A–F) SEM micrographs, contact angle water drop images, and fiber orientation (arrows) of electrospun PLGA groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. SEM scale bar, 500 μm. (G) Fiber diameter and (H) scaffold porosity of untreated and treated PLGA fibers measured by image treatment. Data are expressed as mean±SD. *Significant difference between groups (p<0.05).
Mentions: Structural modifications led to loss of preferential fiber orientation (Fig. 2). Irregular alignment was evident after scaffold hydration, hydrolysis, and FN functionalization (Fig. 2B–F). For the PLGAH01 and PLGAH01FN, porosity results (Fig. 2H) indicated that the space between the fibers became significantly larger, thus generating overlapping sheets on heterogeneous regions of surface (Fig. 2C). Despite of collapsed morphology, no significant difference in fiber diameter was found between PLGAH01 groups and untreated fibers (Fig. 2G). PLGA H001FN showed fibers with a significantly larger diameter compared with both of PLGAH01 groups (Fig. 2G).

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus