Limits...
Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus

SEM micrographs of electrospun PLGA scaffolds and treated groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. Scale bar, 50 μm. Asterisk (*) corresponds to hydrolyzed collapsed fibers; black arrows correspond to deposited structures between PLGA fibers. FN, fibronectin; PBS, phosphate buffered saline; PLGA, poly(d,l-lactide-co-glycolide); SEM, scanning electron microscopy.
© Copyright Policy
Related In: Results  -  Collection


getmorefigures.php?uid=PMC4048976&req=5

f1: SEM micrographs of electrospun PLGA scaffolds and treated groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. Scale bar, 50 μm. Asterisk (*) corresponds to hydrolyzed collapsed fibers; black arrows correspond to deposited structures between PLGA fibers. FN, fibronectin; PBS, phosphate buffered saline; PLGA, poly(d,l-lactide-co-glycolide); SEM, scanning electron microscopy.

Mentions: To propose an optimal framework to PDL regeneration, we performed a co-electrospinning of PLGA solution. PLGA electrospun scaffolds (317±5 μm of thickness) presented highly preferential oriented fibers (Fig. 1A). Structural modification was observed after scaffold hydration in PBS for 24 h at 37°C (Fig. 1B). The morphology of the hydrolyzed fibers differed from the untreated. When treated with higher NaOH concentration (PLGAH01), the fibers became collapsed and broken (Fig. 1C). Samples treated with lower NaOH concentration (PLGAH001) exhibited similar morphology of hydrated nonhydrolyzed scaffolds with relative fiber orientation loss (Fig. 1D). After FN coating, fibers mainly maintained their initial morphology (Fig. 1E,F). It was possible to visualize some thin structures between the PLGA fibers in the PLGAH001FN group (Fig. 1F, black arrows).


Surface Entrapment of Fibronectin on Electrospun PLGA Scaffolds for Periodontal Tissue Engineering.

Campos DM, Gritsch K, Salles V, Attik GN, Grosgogeat B - Biores Open Access (2014)

SEM micrographs of electrospun PLGA scaffolds and treated groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. Scale bar, 50 μm. Asterisk (*) corresponds to hydrolyzed collapsed fibers; black arrows correspond to deposited structures between PLGA fibers. FN, fibronectin; PBS, phosphate buffered saline; PLGA, poly(d,l-lactide-co-glycolide); SEM, scanning electron microscopy.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4048976&req=5

f1: SEM micrographs of electrospun PLGA scaffolds and treated groups: (A) PLGA, (B) PLGA PBS, (C) PLGAH01, (D) PLGAH001, (E) PLGAH01FN, and (F) PLGAH001FN. Scale bar, 50 μm. Asterisk (*) corresponds to hydrolyzed collapsed fibers; black arrows correspond to deposited structures between PLGA fibers. FN, fibronectin; PBS, phosphate buffered saline; PLGA, poly(d,l-lactide-co-glycolide); SEM, scanning electron microscopy.
Mentions: To propose an optimal framework to PDL regeneration, we performed a co-electrospinning of PLGA solution. PLGA electrospun scaffolds (317±5 μm of thickness) presented highly preferential oriented fibers (Fig. 1A). Structural modification was observed after scaffold hydration in PBS for 24 h at 37°C (Fig. 1B). The morphology of the hydrolyzed fibers differed from the untreated. When treated with higher NaOH concentration (PLGAH01), the fibers became collapsed and broken (Fig. 1C). Samples treated with lower NaOH concentration (PLGAH001) exhibited similar morphology of hydrated nonhydrolyzed scaffolds with relative fiber orientation loss (Fig. 1D). After FN coating, fibers mainly maintained their initial morphology (Fig. 1E,F). It was possible to visualize some thin structures between the PLGA fibers in the PLGAH001FN group (Fig. 1F, black arrows).

Bottom Line: Suitable degradation behavior without pH variations was observed for all samples up to 28 days.All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers.However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds.

View Article: PubMed Central - PubMed

Affiliation: Laboratoire des Multimatériaux et Interfaces CNRS UMR 5615, Université Lyon 1 , Villeurbanne, France . ; UFR d'odontologie, Université Lyon 1 , Villeurbanne, France .

ABSTRACT
Nowadays, the challenge in the tissue engineering field consists in the development of biomaterials designed to regenerate ad integrum damaged tissues. Despite the current use of bioresorbable polyesters such as poly(l-lactide) (PLA), poly(d,l-lactide-co-glycolide) (PLGA), and poly-ɛ-caprolactone in soft tissue regeneration researches, their hydrophobic properties negatively influence the cell adhesion. Here, to overcome it, we have developed a fibronectin (FN)-functionalized electrospun PLGA scaffold for periodontal ligament regeneration. Functionalization of electrospun PLGA scaffolds was performed by alkaline hydrolysis (0.1 or 0.01 M NaOH). Then, hydrolyzed scaffolds were coated by simple deposition of an FN layer (10 μg/mL). FN coating was evidenced by X-ray photoelectron analysis. A decrease of contact angle and greater cell adhesion to hydrolyzed, FN-coated PLGA scaffolds were noticed. Suitable degradation behavior without pH variations was observed for all samples up to 28 days. All treated materials presented strong shrinkage, fiber orientation loss, and collapsed fibers. However, functionalization process using 0.01 M NaOH concentration resulted in unchanged scaffold porosity, preserved chemical composition, and similar mechanical properties compared with untreated scaffolds. The proposed simplified method to functionalize electrospun PLGA fibers is an efficient route to make polyester scaffolds more biocompatible and shows potential for tissue engineering.

No MeSH data available.


Related in: MedlinePlus