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Spiral ganglion stem cells can be propagated and differentiated into neurons and glia.

Diensthuber M, Zecha V, Wagenblast J, Arnhold S, Edge AS, Stöver T - Biores Open Access (2014)

Bottom Line: Importantly, spiral ganglion sphere cells maintain their major stem cell characteristics after repeated propagation, which enables the culture of spheres for an extended period of time.In this work, we also demonstrate that differentiated sphere-derived cell populations not only adopt the immunophenotype of mature spiral ganglion cells but also develop distinct ultrastructural features of neurons and glial cells.Thus, our work provides further evidence that self-renewing spiral ganglion stem cells might serve as a promising source for the regeneration of lost auditory neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Frankfurt am Main , Goethe University, Frankfurt am Main, Germany . ; Department of Otology and Laryngology, Harvard Medical School , Boston, Massachusetts. ; Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary , Boston, Massachusetts.

ABSTRACT
The spiral ganglion is an essential functional component of the peripheral auditory system. Most types of hearing loss are associated with spiral ganglion cell degeneration which is irreversible due to the inner ear's lack of regenerative capacity. Recent studies revealed the existence of stem cells in the postnatal spiral ganglion, which gives rise to the hope that these cells might be useful for regenerative inner ear therapies. Here, we provide an in-depth analysis of sphere-forming stem cells isolated from the spiral ganglion of postnatal mice. We show that spiral ganglion spheres have characteristics similar to neurospheres isolated from the brain. Importantly, spiral ganglion sphere cells maintain their major stem cell characteristics after repeated propagation, which enables the culture of spheres for an extended period of time. In this work, we also demonstrate that differentiated sphere-derived cell populations not only adopt the immunophenotype of mature spiral ganglion cells but also develop distinct ultrastructural features of neurons and glial cells. Thus, our work provides further evidence that self-renewing spiral ganglion stem cells might serve as a promising source for the regeneration of lost auditory neurons.

No MeSH data available.


Related in: MedlinePlus

Primary spheres (A) and propagated spheres (B, C) show similar morphology and growth characteristics. (D) Analysis of the size (maximum diameter), the cell number, and the volume of primary, secondary, and tertiary spheres during a 7-day culture period using the neurosphere assay. After DIV 5 tertiary spheres showed a reduced ability to attach to coated culture dishes, which was required for cell counting. The sphere cell number was therefore only analyzed until DIV 5. Data are the mean±SD from three independent experiments. Scale bar=100 μm.
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f5: Primary spheres (A) and propagated spheres (B, C) show similar morphology and growth characteristics. (D) Analysis of the size (maximum diameter), the cell number, and the volume of primary, secondary, and tertiary spheres during a 7-day culture period using the neurosphere assay. After DIV 5 tertiary spheres showed a reduced ability to attach to coated culture dishes, which was required for cell counting. The sphere cell number was therefore only analyzed until DIV 5. Data are the mean±SD from three independent experiments. Scale bar=100 μm.

Mentions: Analysis of primary sphere diameter, cell number, and volume revealed that small cell colonies appear already 1 day after plating the single cells and that these clusters grow continuously throughout the 7-day culture period (Fig. 5A,D). After propagation, the second and third sphere generations showed very similar growth characteristics (Fig. 5B–D).


Spiral ganglion stem cells can be propagated and differentiated into neurons and glia.

Diensthuber M, Zecha V, Wagenblast J, Arnhold S, Edge AS, Stöver T - Biores Open Access (2014)

Primary spheres (A) and propagated spheres (B, C) show similar morphology and growth characteristics. (D) Analysis of the size (maximum diameter), the cell number, and the volume of primary, secondary, and tertiary spheres during a 7-day culture period using the neurosphere assay. After DIV 5 tertiary spheres showed a reduced ability to attach to coated culture dishes, which was required for cell counting. The sphere cell number was therefore only analyzed until DIV 5. Data are the mean±SD from three independent experiments. Scale bar=100 μm.
© Copyright Policy
Related In: Results  -  Collection

Show All Figures
getmorefigures.php?uid=PMC4048968&req=5

f5: Primary spheres (A) and propagated spheres (B, C) show similar morphology and growth characteristics. (D) Analysis of the size (maximum diameter), the cell number, and the volume of primary, secondary, and tertiary spheres during a 7-day culture period using the neurosphere assay. After DIV 5 tertiary spheres showed a reduced ability to attach to coated culture dishes, which was required for cell counting. The sphere cell number was therefore only analyzed until DIV 5. Data are the mean±SD from three independent experiments. Scale bar=100 μm.
Mentions: Analysis of primary sphere diameter, cell number, and volume revealed that small cell colonies appear already 1 day after plating the single cells and that these clusters grow continuously throughout the 7-day culture period (Fig. 5A,D). After propagation, the second and third sphere generations showed very similar growth characteristics (Fig. 5B–D).

Bottom Line: Importantly, spiral ganglion sphere cells maintain their major stem cell characteristics after repeated propagation, which enables the culture of spheres for an extended period of time.In this work, we also demonstrate that differentiated sphere-derived cell populations not only adopt the immunophenotype of mature spiral ganglion cells but also develop distinct ultrastructural features of neurons and glial cells.Thus, our work provides further evidence that self-renewing spiral ganglion stem cells might serve as a promising source for the regeneration of lost auditory neurons.

View Article: PubMed Central - PubMed

Affiliation: Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Frankfurt am Main , Goethe University, Frankfurt am Main, Germany . ; Department of Otology and Laryngology, Harvard Medical School , Boston, Massachusetts. ; Eaton-Peabody Laboratory, Massachusetts Eye and Ear Infirmary , Boston, Massachusetts.

ABSTRACT
The spiral ganglion is an essential functional component of the peripheral auditory system. Most types of hearing loss are associated with spiral ganglion cell degeneration which is irreversible due to the inner ear's lack of regenerative capacity. Recent studies revealed the existence of stem cells in the postnatal spiral ganglion, which gives rise to the hope that these cells might be useful for regenerative inner ear therapies. Here, we provide an in-depth analysis of sphere-forming stem cells isolated from the spiral ganglion of postnatal mice. We show that spiral ganglion spheres have characteristics similar to neurospheres isolated from the brain. Importantly, spiral ganglion sphere cells maintain their major stem cell characteristics after repeated propagation, which enables the culture of spheres for an extended period of time. In this work, we also demonstrate that differentiated sphere-derived cell populations not only adopt the immunophenotype of mature spiral ganglion cells but also develop distinct ultrastructural features of neurons and glial cells. Thus, our work provides further evidence that self-renewing spiral ganglion stem cells might serve as a promising source for the regeneration of lost auditory neurons.

No MeSH data available.


Related in: MedlinePlus