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Damage of photoreceptor-derived cells in culture induced by light emitting diode-derived blue light.

Kuse Y, Ogawa K, Tsuruma K, Shimazawa M, Hara H - Sci Rep (2014)

Bottom Line: Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light.VDTs are equipped with televisions, personal computers, and smart phones.Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)).

View Article: PubMed Central - PubMed

Affiliation: Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

ABSTRACT
Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light. VDTs are equipped with televisions, personal computers, and smart phones. The present study aims to clarify the mechanism underlying blue LED light-induced photoreceptor cell damage. Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)). In the present study, blue LED light increased reactive oxygen species (ROS) production, altered the protein expression level, induced the aggregation of short-wavelength opsins (S-opsin), resulting in severe cell damage. While, blue LED light damaged the primary retinal cells and the damage was photoreceptor specific. N-Acetylcysteine (NAC), an antioxidant, protected against the cellular damage induced by blue LED light. Overall, the LED light induced cell damage was wavelength-, but not energy-dependent and may cause more severe retinal photoreceptor cell damage than the other LED light.

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Related in: MedlinePlus

The putative pathway of blue LED light-induced retinal photoreceptor-derived cell damage.In 661 W cells, blue LED light induces ROS production and S-opsin aggregation. The rapid ROS increase leads to mitochondrial damage and the MAPK activation or the nuclear translocation of NF-κB. Activated MAPK and NF-κB induces the activation of caspase and leads to apoptotic cell death. Active NF-κB also activates autophagy, and excessive autophagy leads to cell death. While, S-opsin aggregation causes endoplasmic reticulum (ER) stress. Blue LED light-induced retinal photoreceptor-derived cell death may be associated with both oxidative stress and ER stress.
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f8: The putative pathway of blue LED light-induced retinal photoreceptor-derived cell damage.In 661 W cells, blue LED light induces ROS production and S-opsin aggregation. The rapid ROS increase leads to mitochondrial damage and the MAPK activation or the nuclear translocation of NF-κB. Activated MAPK and NF-κB induces the activation of caspase and leads to apoptotic cell death. Active NF-κB also activates autophagy, and excessive autophagy leads to cell death. While, S-opsin aggregation causes endoplasmic reticulum (ER) stress. Blue LED light-induced retinal photoreceptor-derived cell death may be associated with both oxidative stress and ER stress.

Mentions: In the present study, we demonstrated that the in vitro exposure to blue LED light damaged the 661 W cells more severely compared to white or green LED light. In primary retinal cell culture, blue LED light damaged retinal photoreceptor cells. Blue LED light-induced 661 W cell damage was associated with rapid ROS increase, NF-κB activation, p38 activation, ERK 1/2 inactivation, S-opsin aggregation, and activated caspase-3/7 and autophagy (Figure 8).


Damage of photoreceptor-derived cells in culture induced by light emitting diode-derived blue light.

Kuse Y, Ogawa K, Tsuruma K, Shimazawa M, Hara H - Sci Rep (2014)

The putative pathway of blue LED light-induced retinal photoreceptor-derived cell damage.In 661 W cells, blue LED light induces ROS production and S-opsin aggregation. The rapid ROS increase leads to mitochondrial damage and the MAPK activation or the nuclear translocation of NF-κB. Activated MAPK and NF-κB induces the activation of caspase and leads to apoptotic cell death. Active NF-κB also activates autophagy, and excessive autophagy leads to cell death. While, S-opsin aggregation causes endoplasmic reticulum (ER) stress. Blue LED light-induced retinal photoreceptor-derived cell death may be associated with both oxidative stress and ER stress.
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048889&req=5

f8: The putative pathway of blue LED light-induced retinal photoreceptor-derived cell damage.In 661 W cells, blue LED light induces ROS production and S-opsin aggregation. The rapid ROS increase leads to mitochondrial damage and the MAPK activation or the nuclear translocation of NF-κB. Activated MAPK and NF-κB induces the activation of caspase and leads to apoptotic cell death. Active NF-κB also activates autophagy, and excessive autophagy leads to cell death. While, S-opsin aggregation causes endoplasmic reticulum (ER) stress. Blue LED light-induced retinal photoreceptor-derived cell death may be associated with both oxidative stress and ER stress.
Mentions: In the present study, we demonstrated that the in vitro exposure to blue LED light damaged the 661 W cells more severely compared to white or green LED light. In primary retinal cell culture, blue LED light damaged retinal photoreceptor cells. Blue LED light-induced 661 W cell damage was associated with rapid ROS increase, NF-κB activation, p38 activation, ERK 1/2 inactivation, S-opsin aggregation, and activated caspase-3/7 and autophagy (Figure 8).

Bottom Line: Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light.VDTs are equipped with televisions, personal computers, and smart phones.Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)).

View Article: PubMed Central - PubMed

Affiliation: Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

ABSTRACT
Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light. VDTs are equipped with televisions, personal computers, and smart phones. The present study aims to clarify the mechanism underlying blue LED light-induced photoreceptor cell damage. Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)). In the present study, blue LED light increased reactive oxygen species (ROS) production, altered the protein expression level, induced the aggregation of short-wavelength opsins (S-opsin), resulting in severe cell damage. While, blue LED light damaged the primary retinal cells and the damage was photoreceptor specific. N-Acetylcysteine (NAC), an antioxidant, protected against the cellular damage induced by blue LED light. Overall, the LED light induced cell damage was wavelength-, but not energy-dependent and may cause more severe retinal photoreceptor cell damage than the other LED light.

Show MeSH
Related in: MedlinePlus