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Damage of photoreceptor-derived cells in culture induced by light emitting diode-derived blue light.

Kuse Y, Ogawa K, Tsuruma K, Shimazawa M, Hara H - Sci Rep (2014)

Bottom Line: Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light.VDTs are equipped with televisions, personal computers, and smart phones.Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)).

View Article: PubMed Central - PubMed

Affiliation: Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

ABSTRACT
Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light. VDTs are equipped with televisions, personal computers, and smart phones. The present study aims to clarify the mechanism underlying blue LED light-induced photoreceptor cell damage. Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)). In the present study, blue LED light increased reactive oxygen species (ROS) production, altered the protein expression level, induced the aggregation of short-wavelength opsins (S-opsin), resulting in severe cell damage. While, blue LED light damaged the primary retinal cells and the damage was photoreceptor specific. N-Acetylcysteine (NAC), an antioxidant, protected against the cellular damage induced by blue LED light. Overall, the LED light induced cell damage was wavelength-, but not energy-dependent and may cause more severe retinal photoreceptor cell damage than the other LED light.

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Related in: MedlinePlus

The effects of blue, white, and green LED lights on the cell viability.(A) The exposure of blue, white, and green LED light to cells cultured in a 96-well plate. (B) The observation of cell morphology using bright field microscopy, showing blue LED light caused the morphological changes compared with the control. Green LED light did not change the cells. (C–E) The quantitative evaluation of cell viability by the CCK-8 assay. This result is consistent with the observed change in cell morphology. Cell viability was reduced by blue and white LED light exposure, but not green LED light. The scale bar represents 50 μm. Data are expressed as mean ± SEM (n = 6). ## indicates p < 0.01 vs. control (ANOVA).
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f1: The effects of blue, white, and green LED lights on the cell viability.(A) The exposure of blue, white, and green LED light to cells cultured in a 96-well plate. (B) The observation of cell morphology using bright field microscopy, showing blue LED light caused the morphological changes compared with the control. Green LED light did not change the cells. (C–E) The quantitative evaluation of cell viability by the CCK-8 assay. This result is consistent with the observed change in cell morphology. Cell viability was reduced by blue and white LED light exposure, but not green LED light. The scale bar represents 50 μm. Data are expressed as mean ± SEM (n = 6). ## indicates p < 0.01 vs. control (ANOVA).

Mentions: We first examined the relationship between the photoreceptor-derived cell damage and the difference in the color of the LED lights, under the same illuminance of 2,500 lux. Our results suggested that the blue LED light damaged the photoreceptor-derived cells more severely than white and green LED light (see Supplementary Fig. S1 online). Next, we investigated the effects of LED light on the cells under unified energy (0.38 mW/cm2). This energy equals to 2,500 lux of green LED light. The photograph illustrates 96 well plates exposed to each LED light (Figure 1A). A representative photomicrograph of cell morphology was taken using bright field microscopy. The photoreceptor-derived cells were changed by blue and white LED light (Figure 1B). Green LED light did not change the cells. Quantitative data showed blue and white LED lights significantly reduced cell viability, but green LED light did not affect it (Figure 1C–E).


Damage of photoreceptor-derived cells in culture induced by light emitting diode-derived blue light.

Kuse Y, Ogawa K, Tsuruma K, Shimazawa M, Hara H - Sci Rep (2014)

The effects of blue, white, and green LED lights on the cell viability.(A) The exposure of blue, white, and green LED light to cells cultured in a 96-well plate. (B) The observation of cell morphology using bright field microscopy, showing blue LED light caused the morphological changes compared with the control. Green LED light did not change the cells. (C–E) The quantitative evaluation of cell viability by the CCK-8 assay. This result is consistent with the observed change in cell morphology. Cell viability was reduced by blue and white LED light exposure, but not green LED light. The scale bar represents 50 μm. Data are expressed as mean ± SEM (n = 6). ## indicates p < 0.01 vs. control (ANOVA).
© Copyright Policy - open-access
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048889&req=5

f1: The effects of blue, white, and green LED lights on the cell viability.(A) The exposure of blue, white, and green LED light to cells cultured in a 96-well plate. (B) The observation of cell morphology using bright field microscopy, showing blue LED light caused the morphological changes compared with the control. Green LED light did not change the cells. (C–E) The quantitative evaluation of cell viability by the CCK-8 assay. This result is consistent with the observed change in cell morphology. Cell viability was reduced by blue and white LED light exposure, but not green LED light. The scale bar represents 50 μm. Data are expressed as mean ± SEM (n = 6). ## indicates p < 0.01 vs. control (ANOVA).
Mentions: We first examined the relationship between the photoreceptor-derived cell damage and the difference in the color of the LED lights, under the same illuminance of 2,500 lux. Our results suggested that the blue LED light damaged the photoreceptor-derived cells more severely than white and green LED light (see Supplementary Fig. S1 online). Next, we investigated the effects of LED light on the cells under unified energy (0.38 mW/cm2). This energy equals to 2,500 lux of green LED light. The photograph illustrates 96 well plates exposed to each LED light (Figure 1A). A representative photomicrograph of cell morphology was taken using bright field microscopy. The photoreceptor-derived cells were changed by blue and white LED light (Figure 1B). Green LED light did not change the cells. Quantitative data showed blue and white LED lights significantly reduced cell viability, but green LED light did not affect it (Figure 1C–E).

Bottom Line: Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light.VDTs are equipped with televisions, personal computers, and smart phones.Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)).

View Article: PubMed Central - PubMed

Affiliation: Molecular Pharmacology, Department of Biofunctional Evaluation, Gifu Pharmaceutical University, 1-25-4 Daigaku-nishi, Gifu 501-1196, Japan.

ABSTRACT
Our eyes are increasingly exposed to light from the emitting diode (LED) light of video display terminals (VDT) which contain much blue light. VDTs are equipped with televisions, personal computers, and smart phones. The present study aims to clarify the mechanism underlying blue LED light-induced photoreceptor cell damage. Murine cone photoreceptor-derived cells (661 W) were exposed to blue, white, or green LED light (0.38 mW/cm(2)). In the present study, blue LED light increased reactive oxygen species (ROS) production, altered the protein expression level, induced the aggregation of short-wavelength opsins (S-opsin), resulting in severe cell damage. While, blue LED light damaged the primary retinal cells and the damage was photoreceptor specific. N-Acetylcysteine (NAC), an antioxidant, protected against the cellular damage induced by blue LED light. Overall, the LED light induced cell damage was wavelength-, but not energy-dependent and may cause more severe retinal photoreceptor cell damage than the other LED light.

Show MeSH
Related in: MedlinePlus