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Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice.

Nakatsuji H, Kishida K, Sekimoto R, Funahashi T, Shimomura I - FEBS Open Bio (2014)

Bottom Line: Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis.In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7.In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.

ABSTRACT
Adiponectin is exclusively synthesized by adipocytes and exhibits anti-diabetic, anti-atherosclerotic and anti-inflammatory properties. Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis. However, the mechanisms responsible for hypoadiponectinemia remain unclear. Here, we investigated adiponectin movement using hetero parabiosis model of wild type (WT) and adiponectin-deficient (KO) mice. WT mice were parabiosed with WT mice (WT-WT) or KO mice (WT-KO) and adiponectin levels were measured serially up to 63 days after surgery. In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7. Circulating adiponectin levels decreased further to the detection limit of assay, and remained low up to day 63. However, adiponectin protein was detected in the adipose tissues of not only the WT partner but also WT-KO mice. In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins. The use of parabiosis experiments shed light on movement of native adiponectin among different tissues such as the state of hypoadiponectinemia in obesity.

No MeSH data available.


Related in: MedlinePlus

Accumulation of adiponectin in SVF of obese KO mice. (A) WAT tissue weight in parabiosis mice. (B) Hematoxylin and eosin-stained sections and adipocyte cell size distribution. Scale bar: 100 μm. (C) Adiponectin levels in MAF and SVF of adipose tissues. Western blot analysis (top). Quantitative data measured using adiponectin ELISA kit (n = 4–6, each group, bottom). (D) Gene expression of adiponectin and its binding molecules and receptors in MAF and SVF (n = 4–6). Data are mean ± SEM. Each experiment was repeated at least three times.
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f0020: Accumulation of adiponectin in SVF of obese KO mice. (A) WAT tissue weight in parabiosis mice. (B) Hematoxylin and eosin-stained sections and adipocyte cell size distribution. Scale bar: 100 μm. (C) Adiponectin levels in MAF and SVF of adipose tissues. Western blot analysis (top). Quantitative data measured using adiponectin ELISA kit (n = 4–6, each group, bottom). (D) Gene expression of adiponectin and its binding molecules and receptors in MAF and SVF (n = 4–6). Data are mean ± SEM. Each experiment was repeated at least three times.

Mentions: Movement of circulating adiponectin levels in HF/HS-fed mice was similar to that in NC-fed mice. At day 63, tissue weights of WATsub and WATmes of mice fed HF/HS diet was significantly higher than of mice fed NC diet (Fig. 4A). There was no significant difference of tissue weight of WAT between WT and KO partners fed both diets (Fig. 4A). Histological examination showed that adipocytes in WATsub and WATmes of HF/HS-fed mice were larger than those of NC-fed mice (Fig. 4B).


Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice.

Nakatsuji H, Kishida K, Sekimoto R, Funahashi T, Shimomura I - FEBS Open Bio (2014)

Accumulation of adiponectin in SVF of obese KO mice. (A) WAT tissue weight in parabiosis mice. (B) Hematoxylin and eosin-stained sections and adipocyte cell size distribution. Scale bar: 100 μm. (C) Adiponectin levels in MAF and SVF of adipose tissues. Western blot analysis (top). Quantitative data measured using adiponectin ELISA kit (n = 4–6, each group, bottom). (D) Gene expression of adiponectin and its binding molecules and receptors in MAF and SVF (n = 4–6). Data are mean ± SEM. Each experiment was repeated at least three times.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048846&req=5

f0020: Accumulation of adiponectin in SVF of obese KO mice. (A) WAT tissue weight in parabiosis mice. (B) Hematoxylin and eosin-stained sections and adipocyte cell size distribution. Scale bar: 100 μm. (C) Adiponectin levels in MAF and SVF of adipose tissues. Western blot analysis (top). Quantitative data measured using adiponectin ELISA kit (n = 4–6, each group, bottom). (D) Gene expression of adiponectin and its binding molecules and receptors in MAF and SVF (n = 4–6). Data are mean ± SEM. Each experiment was repeated at least three times.
Mentions: Movement of circulating adiponectin levels in HF/HS-fed mice was similar to that in NC-fed mice. At day 63, tissue weights of WATsub and WATmes of mice fed HF/HS diet was significantly higher than of mice fed NC diet (Fig. 4A). There was no significant difference of tissue weight of WAT between WT and KO partners fed both diets (Fig. 4A). Histological examination showed that adipocytes in WATsub and WATmes of HF/HS-fed mice were larger than those of NC-fed mice (Fig. 4B).

Bottom Line: Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis.In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7.In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.

ABSTRACT
Adiponectin is exclusively synthesized by adipocytes and exhibits anti-diabetic, anti-atherosclerotic and anti-inflammatory properties. Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis. However, the mechanisms responsible for hypoadiponectinemia remain unclear. Here, we investigated adiponectin movement using hetero parabiosis model of wild type (WT) and adiponectin-deficient (KO) mice. WT mice were parabiosed with WT mice (WT-WT) or KO mice (WT-KO) and adiponectin levels were measured serially up to 63 days after surgery. In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7. Circulating adiponectin levels decreased further to the detection limit of assay, and remained low up to day 63. However, adiponectin protein was detected in the adipose tissues of not only the WT partner but also WT-KO mice. In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins. The use of parabiosis experiments shed light on movement of native adiponectin among different tissues such as the state of hypoadiponectinemia in obesity.

No MeSH data available.


Related in: MedlinePlus