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Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice.

Nakatsuji H, Kishida K, Sekimoto R, Funahashi T, Shimomura I - FEBS Open Bio (2014)

Bottom Line: Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis.In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7.In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.

ABSTRACT
Adiponectin is exclusively synthesized by adipocytes and exhibits anti-diabetic, anti-atherosclerotic and anti-inflammatory properties. Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis. However, the mechanisms responsible for hypoadiponectinemia remain unclear. Here, we investigated adiponectin movement using hetero parabiosis model of wild type (WT) and adiponectin-deficient (KO) mice. WT mice were parabiosed with WT mice (WT-WT) or KO mice (WT-KO) and adiponectin levels were measured serially up to 63 days after surgery. In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7. Circulating adiponectin levels decreased further to the detection limit of assay, and remained low up to day 63. However, adiponectin protein was detected in the adipose tissues of not only the WT partner but also WT-KO mice. In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins. The use of parabiosis experiments shed light on movement of native adiponectin among different tissues such as the state of hypoadiponectinemia in obesity.

No MeSH data available.


Related in: MedlinePlus

Accumulation of adiponectin in WAT of parabiosis mice. (A) WAT tissue weight in parabiosis mice. WATmes; mesenteric white adipose tissue, WATsub; subcutaneous white adipose tissue. n.s.; not significant. (B) Adiponectin protein levels in various tissues. Western blot analysis (top). Quantitative data measured by ELISA (bottom). Data of KO (WT–KO) are also shown in enlarged view. (C) Gene expression levels of adiponectin and its binding molecules or receptors in WAT. (n = 4–6, each group). ND; not detected. Data are mean ± SEM. Each experiment was repeated at least three times.
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f0010: Accumulation of adiponectin in WAT of parabiosis mice. (A) WAT tissue weight in parabiosis mice. WATmes; mesenteric white adipose tissue, WATsub; subcutaneous white adipose tissue. n.s.; not significant. (B) Adiponectin protein levels in various tissues. Western blot analysis (top). Quantitative data measured by ELISA (bottom). Data of KO (WT–KO) are also shown in enlarged view. (C) Gene expression levels of adiponectin and its binding molecules or receptors in WAT. (n = 4–6, each group). ND; not detected. Data are mean ± SEM. Each experiment was repeated at least three times.

Mentions: At day 63 after surgical parabiosis, each pair mice was sacrificed and analyzed. There was no significant difference in tissue weight of WATsub and WATmes between WT (WT–WT), WT (WT–KO) and KO (WT–KO) (Fig. 2A). Next, we examined the tissue distribution of adiponectin protein in these mice. Both western blotting and tissue ELISA assay showed abundant adiponectin protein levels in WATsub, WATmes and aorta of WT (WT–KO), similar to WT (WT–WT) (Fig. 2B). However, lower adiponectin protein levels were detected in WATsub, WATmes and aorta of KO (WT–KO) (Fig. 2B), which lacks adiponectin mRNA expression (Fig. 2C).


Tracing the movement of adiponectin in a parabiosis model of wild-type and adiponectin-knockout mice.

Nakatsuji H, Kishida K, Sekimoto R, Funahashi T, Shimomura I - FEBS Open Bio (2014)

Accumulation of adiponectin in WAT of parabiosis mice. (A) WAT tissue weight in parabiosis mice. WATmes; mesenteric white adipose tissue, WATsub; subcutaneous white adipose tissue. n.s.; not significant. (B) Adiponectin protein levels in various tissues. Western blot analysis (top). Quantitative data measured by ELISA (bottom). Data of KO (WT–KO) are also shown in enlarged view. (C) Gene expression levels of adiponectin and its binding molecules or receptors in WAT. (n = 4–6, each group). ND; not detected. Data are mean ± SEM. Each experiment was repeated at least three times.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048846&req=5

f0010: Accumulation of adiponectin in WAT of parabiosis mice. (A) WAT tissue weight in parabiosis mice. WATmes; mesenteric white adipose tissue, WATsub; subcutaneous white adipose tissue. n.s.; not significant. (B) Adiponectin protein levels in various tissues. Western blot analysis (top). Quantitative data measured by ELISA (bottom). Data of KO (WT–KO) are also shown in enlarged view. (C) Gene expression levels of adiponectin and its binding molecules or receptors in WAT. (n = 4–6, each group). ND; not detected. Data are mean ± SEM. Each experiment was repeated at least three times.
Mentions: At day 63 after surgical parabiosis, each pair mice was sacrificed and analyzed. There was no significant difference in tissue weight of WATsub and WATmes between WT (WT–WT), WT (WT–KO) and KO (WT–KO) (Fig. 2A). Next, we examined the tissue distribution of adiponectin protein in these mice. Both western blotting and tissue ELISA assay showed abundant adiponectin protein levels in WATsub, WATmes and aorta of WT (WT–KO), similar to WT (WT–WT) (Fig. 2B). However, lower adiponectin protein levels were detected in WATsub, WATmes and aorta of KO (WT–KO) (Fig. 2B), which lacks adiponectin mRNA expression (Fig. 2C).

Bottom Line: Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis.In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7.In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins.

View Article: PubMed Central - PubMed

Affiliation: Department of Metabolic Medicine, Graduate School of Medicine, Osaka University, Osaka, Japan.

ABSTRACT
Adiponectin is exclusively synthesized by adipocytes and exhibits anti-diabetic, anti-atherosclerotic and anti-inflammatory properties. Hypoadiponectinemia is associated in obese individuals with insulin resistance and atherosclerosis. However, the mechanisms responsible for hypoadiponectinemia remain unclear. Here, we investigated adiponectin movement using hetero parabiosis model of wild type (WT) and adiponectin-deficient (KO) mice. WT mice were parabiosed with WT mice (WT-WT) or KO mice (WT-KO) and adiponectin levels were measured serially up to 63 days after surgery. In the WT-KO parabiosis model, circulating adiponectin levels of the WT partners decreased rapidly, on the other hand, those of KO partners increased, and then these reached comparable levels each other at day 7. Circulating adiponectin levels decreased further to the detection limit of assay, and remained low up to day 63. However, adiponectin protein was detected in the adipose tissues of not only the WT partner but also WT-KO mice. In the diet-induced obesity model, high adiponectin protein levels were detected in adipose stromal vascular fraction of diet-induced obese KO partner, without changes in its binding proteins. The use of parabiosis experiments shed light on movement of native adiponectin among different tissues such as the state of hypoadiponectinemia in obesity.

No MeSH data available.


Related in: MedlinePlus