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Nonlinear photoacoustic signal amplification from single targets in absorption background.

Sarimollaoglu M, Nedosekin DA, Menyaev YA, Juratli MA, Zharov VP - Photoacoustics (2014)

Bottom Line: This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo.Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background.Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

View Article: PubMed Central - PubMed

Affiliation: Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR USA 72205.

ABSTRACT
Photoacoustic (PA) detection of single absorbing targets such as nanoparticles or cells can be limited by absorption background. We show here that this problem can be overcome by using the nonlinear photoacoustics based on the differences in PA signal dependences on the laser energy from targets and background. Among different nonlinear phenomena, we focused on laser generation of nanobubbles as more efficient PA signal amplifiers from strongly absorbing, highly localized targets in the presence of spatially homogenous absorption background generating linear signals only. This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo. Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background. Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

No MeSH data available.


Related in: MedlinePlus

PA signal amplitudes from single melanoma cells as function of laser pulse number, at laser energy fluence of 415 mJ/cm2 (a) and 805 mJ/cm2 (b). Bars are mean ± SEM.
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fig0020: PA signal amplitudes from single melanoma cells as function of laser pulse number, at laser energy fluence of 415 mJ/cm2 (a) and 805 mJ/cm2 (b). Bars are mean ± SEM.

Mentions: For the cells in PBS, PA signal amplitudes were consistent from pulse to pulse at fluence of 415 mJ/cm2 and below (Fig. 4a). However, at higher fluence of 805 mJ/cm2, PA signals quickly diminished (Fig. 4b) due to PT-based bleaching. This effect was more profound for high-pigmented cells. In particular, PA signals from high-pigmented cells became even lower than the less pigmented cells after 20 laser pulses.


Nonlinear photoacoustic signal amplification from single targets in absorption background.

Sarimollaoglu M, Nedosekin DA, Menyaev YA, Juratli MA, Zharov VP - Photoacoustics (2014)

PA signal amplitudes from single melanoma cells as function of laser pulse number, at laser energy fluence of 415 mJ/cm2 (a) and 805 mJ/cm2 (b). Bars are mean ± SEM.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048727&req=5

fig0020: PA signal amplitudes from single melanoma cells as function of laser pulse number, at laser energy fluence of 415 mJ/cm2 (a) and 805 mJ/cm2 (b). Bars are mean ± SEM.
Mentions: For the cells in PBS, PA signal amplitudes were consistent from pulse to pulse at fluence of 415 mJ/cm2 and below (Fig. 4a). However, at higher fluence of 805 mJ/cm2, PA signals quickly diminished (Fig. 4b) due to PT-based bleaching. This effect was more profound for high-pigmented cells. In particular, PA signals from high-pigmented cells became even lower than the less pigmented cells after 20 laser pulses.

Bottom Line: This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo.Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background.Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

View Article: PubMed Central - PubMed

Affiliation: Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR USA 72205.

ABSTRACT
Photoacoustic (PA) detection of single absorbing targets such as nanoparticles or cells can be limited by absorption background. We show here that this problem can be overcome by using the nonlinear photoacoustics based on the differences in PA signal dependences on the laser energy from targets and background. Among different nonlinear phenomena, we focused on laser generation of nanobubbles as more efficient PA signal amplifiers from strongly absorbing, highly localized targets in the presence of spatially homogenous absorption background generating linear signals only. This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo. Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background. Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

No MeSH data available.


Related in: MedlinePlus