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Nonlinear photoacoustic signal amplification from single targets in absorption background.

Sarimollaoglu M, Nedosekin DA, Menyaev YA, Juratli MA, Zharov VP - Photoacoustics (2014)

Bottom Line: This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo.Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background.Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

View Article: PubMed Central - PubMed

Affiliation: Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR USA 72205.

ABSTRACT
Photoacoustic (PA) detection of single absorbing targets such as nanoparticles or cells can be limited by absorption background. We show here that this problem can be overcome by using the nonlinear photoacoustics based on the differences in PA signal dependences on the laser energy from targets and background. Among different nonlinear phenomena, we focused on laser generation of nanobubbles as more efficient PA signal amplifiers from strongly absorbing, highly localized targets in the presence of spatially homogenous absorption background generating linear signals only. This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo. Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background. Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

No MeSH data available.


Related in: MedlinePlus

B16F10 mouse melanoma cells at various resolutions. (a and b) Cells with various melanin content suspended in PBS. (c) Blood spiked with melanoma cells. PT images and signals from single melanoma cells with low (d and f) and high (e and g) melanin content. (h) TEM image of a melanoma cell. (i) Closer view of a small melanosome. Amplitude, time scale, laser wavelength, and fluence: (f) 20 mV/div, 4 μs/div, 580 nm, and 80 mJ/cm2; (g) 50 mV/div, 4 μs/div, 580 nm, and 0.2 J/cm2.
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fig0010: B16F10 mouse melanoma cells at various resolutions. (a and b) Cells with various melanin content suspended in PBS. (c) Blood spiked with melanoma cells. PT images and signals from single melanoma cells with low (d and f) and high (e and g) melanin content. (h) TEM image of a melanoma cell. (i) Closer view of a small melanosome. Amplitude, time scale, laser wavelength, and fluence: (f) 20 mV/div, 4 μs/div, 580 nm, and 80 mJ/cm2; (g) 50 mV/div, 4 μs/div, 580 nm, and 0.2 J/cm2.

Mentions: The amount of melanin in melanoma cells widely varies (Fig. 2a–c) depending on several factors (cell line, growing methods, culturing time, etc.). Before proceeding with further study of nonlinear photoacoustics, the melanin-related pigmentation was evaluated at single cell level. We applied PT/PA methods to measure PT/PA signal amplitudes at the same energy as indicator of melanin content. Specifically, the PT thermal lens signal amplitude and PT image structure in individual live cells were used as indicators of average melanin content (through average cell absorption) and melanin cellular spatial distribution, respectively.


Nonlinear photoacoustic signal amplification from single targets in absorption background.

Sarimollaoglu M, Nedosekin DA, Menyaev YA, Juratli MA, Zharov VP - Photoacoustics (2014)

B16F10 mouse melanoma cells at various resolutions. (a and b) Cells with various melanin content suspended in PBS. (c) Blood spiked with melanoma cells. PT images and signals from single melanoma cells with low (d and f) and high (e and g) melanin content. (h) TEM image of a melanoma cell. (i) Closer view of a small melanosome. Amplitude, time scale, laser wavelength, and fluence: (f) 20 mV/div, 4 μs/div, 580 nm, and 80 mJ/cm2; (g) 50 mV/div, 4 μs/div, 580 nm, and 0.2 J/cm2.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

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fig0010: B16F10 mouse melanoma cells at various resolutions. (a and b) Cells with various melanin content suspended in PBS. (c) Blood spiked with melanoma cells. PT images and signals from single melanoma cells with low (d and f) and high (e and g) melanin content. (h) TEM image of a melanoma cell. (i) Closer view of a small melanosome. Amplitude, time scale, laser wavelength, and fluence: (f) 20 mV/div, 4 μs/div, 580 nm, and 80 mJ/cm2; (g) 50 mV/div, 4 μs/div, 580 nm, and 0.2 J/cm2.
Mentions: The amount of melanin in melanoma cells widely varies (Fig. 2a–c) depending on several factors (cell line, growing methods, culturing time, etc.). Before proceeding with further study of nonlinear photoacoustics, the melanin-related pigmentation was evaluated at single cell level. We applied PT/PA methods to measure PT/PA signal amplitudes at the same energy as indicator of melanin content. Specifically, the PT thermal lens signal amplitude and PT image structure in individual live cells were used as indicators of average melanin content (through average cell absorption) and melanin cellular spatial distribution, respectively.

Bottom Line: This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo.Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background.Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

View Article: PubMed Central - PubMed

Affiliation: Phillips Classic Laser and Nanomedicine Laboratories, Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, 4301 W. Markham St., Little Rock, AR USA 72205.

ABSTRACT
Photoacoustic (PA) detection of single absorbing targets such as nanoparticles or cells can be limited by absorption background. We show here that this problem can be overcome by using the nonlinear photoacoustics based on the differences in PA signal dependences on the laser energy from targets and background. Among different nonlinear phenomena, we focused on laser generation of nanobubbles as more efficient PA signal amplifiers from strongly absorbing, highly localized targets in the presence of spatially homogenous absorption background generating linear signals only. This approach was demonstrated by using nonlinear PA flow cytometry platform for label-free detection of circulating melanoma cells in blood background in vitro and in vivo. Nonlinearly amplified PA signals from overheated melanin nanoclusters in melanoma cells became detectable above still linear blood background. Nonlinear nanobubble-based photoacoustics provide new opportunities to significantly (5-20-fold) increase PA contrast of single nanoparticles, cells, viruses and bacteria in complex biological environments.

No MeSH data available.


Related in: MedlinePlus