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Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography.

Satzer P, Wellhoefer M, Jungbauer A - J Chromatogr A (2014)

Bottom Line: In the case of beta casein where no multimers are present we achieved 89% purity and 90% recovery of loaded nanoparticles in the Raffinate and an extract free of particles (92% purity).Using a tangential flow filtration unit with 5kDa cutoff membrane we proved that the extract can be concentrated for recycling of protein and buffer.The calculated space-time-yield for loaded nanoparticles was 0.25g of loaded nanoparticles per hour and liter of used resin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.

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Timetrace of (●) Retentate protein concentration and (○) Permeate protein concentration of TFF concentration of SEC–SMB extract.
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fig0035: Timetrace of (●) Retentate protein concentration and (○) Permeate protein concentration of TFF concentration of SEC–SMB extract.

Mentions: To prove the applicability of TFF for concentration of protein and reuse of buffer we collected the extract of the SMB–SEC separation of 70 nm silica nanoparticles and beta-casein and concentrated it over the course of 80 min (Fig. 7). We achieved a total concentration factor of 2.8, which allows this solution to be used again for loading of nanoparticles, reaching a yield of 96%. We also proved that the resulting buffer is free of protein and ready to be used in the SMB system again (Fig. 7).


Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography.

Satzer P, Wellhoefer M, Jungbauer A - J Chromatogr A (2014)

Timetrace of (●) Retentate protein concentration and (○) Permeate protein concentration of TFF concentration of SEC–SMB extract.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048465&req=5

fig0035: Timetrace of (●) Retentate protein concentration and (○) Permeate protein concentration of TFF concentration of SEC–SMB extract.
Mentions: To prove the applicability of TFF for concentration of protein and reuse of buffer we collected the extract of the SMB–SEC separation of 70 nm silica nanoparticles and beta-casein and concentrated it over the course of 80 min (Fig. 7). We achieved a total concentration factor of 2.8, which allows this solution to be used again for loading of nanoparticles, reaching a yield of 96%. We also proved that the resulting buffer is free of protein and ready to be used in the SMB system again (Fig. 7).

Bottom Line: In the case of beta casein where no multimers are present we achieved 89% purity and 90% recovery of loaded nanoparticles in the Raffinate and an extract free of particles (92% purity).Using a tangential flow filtration unit with 5kDa cutoff membrane we proved that the extract can be concentrated for recycling of protein and buffer.The calculated space-time-yield for loaded nanoparticles was 0.25g of loaded nanoparticles per hour and liter of used resin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.

Show MeSH
Related in: MedlinePlus