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Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography.

Satzer P, Wellhoefer M, Jungbauer A - J Chromatogr A (2014)

Bottom Line: In the case of beta casein where no multimers are present we achieved 89% purity and 90% recovery of loaded nanoparticles in the Raffinate and an extract free of particles (92% purity).Using a tangential flow filtration unit with 5kDa cutoff membrane we proved that the extract can be concentrated for recycling of protein and buffer.The calculated space-time-yield for loaded nanoparticles was 0.25g of loaded nanoparticles per hour and liter of used resin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.

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m2/m3 plane under the assumption of linear isotherms.
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fig0010: m2/m3 plane under the assumption of linear isotherms.

Mentions: To find optimal parameters Mazotti et al. suggested to fix the values of m1 and m4 and to explore the m2/m3 plane to find optimal parameters, reducing the complex problem of interconnected parameters to a 2D plot. A representation of the restrictions found by Eqs. (2)–(5) is the triangle (hence the name triangle theory) build up by the m2/m3 plane (Fig. 2).


Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography.

Satzer P, Wellhoefer M, Jungbauer A - J Chromatogr A (2014)

m2/m3 plane under the assumption of linear isotherms.
© Copyright Policy - CC BY-NC-ND
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048465&req=5

fig0010: m2/m3 plane under the assumption of linear isotherms.
Mentions: To find optimal parameters Mazotti et al. suggested to fix the values of m1 and m4 and to explore the m2/m3 plane to find optimal parameters, reducing the complex problem of interconnected parameters to a 2D plot. A representation of the restrictions found by Eqs. (2)–(5) is the triangle (hence the name triangle theory) build up by the m2/m3 plane (Fig. 2).

Bottom Line: In the case of beta casein where no multimers are present we achieved 89% purity and 90% recovery of loaded nanoparticles in the Raffinate and an extract free of particles (92% purity).Using a tangential flow filtration unit with 5kDa cutoff membrane we proved that the extract can be concentrated for recycling of protein and buffer.The calculated space-time-yield for loaded nanoparticles was 0.25g of loaded nanoparticles per hour and liter of used resin.

View Article: PubMed Central - PubMed

Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.

Show MeSH
Related in: MedlinePlus