Continuous separation of protein loaded nanoparticles by simulated moving bed chromatography.
Bottom Line: In the case of beta casein where no multimers are present we achieved 89% purity and 90% recovery of loaded nanoparticles in the Raffinate and an extract free of particles (92% purity).Using a tangential flow filtration unit with 5kDa cutoff membrane we proved that the extract can be concentrated for recycling of protein and buffer.The calculated space-time-yield for loaded nanoparticles was 0.25g of loaded nanoparticles per hour and liter of used resin.
Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.Show MeSH
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Mentions: Optimal operation conditions for an SMB system can be hard to determine empirically because of many adjustable and interconnected parameters. Different methods have been proposed to estimate suitable conditions for SMB. Ruthven and Ching  proposed a method later refined by Guiochon  which assumes linear isotherms but has to apply a safety margin on the modelled parameters to account for inaccuracy. An alternative process to find suitable parameters using equilibrium constants was presented by Storti and Mazzotti  and was termed triangle theory. For this model they also assumed linear adsorption isotherms, but also made the assumption that axial dispersion and mass transfer resistance are negligible. Later on, the triangle theory was refined by Mazotti et al.  and we based our parameters on the equation in this paper. The triangle theory works by defining a ratio for each of the four sections in the SMB, assigning different tasks to each section (Fig. 1). Zone 1 regenerates the chromatographic resin and section 4 regenerates the solvent, where section 2 and 3 are separating 2 species in a binary mixture.
Affiliation: Department of Biotechnology, University of Natural Resources and Life Sciences, Vienna (BOKU), Muthgasse 18, 1190 Vienna, Austria.