Quantitative temporal viromics: an approach to investigate host-pathogen interaction.
Bottom Line: QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets.Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined.QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: email@example.com.Show MeSH
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Mentions: Most prior studies of the temporal expression of canonical HCMV proteins have employed immunoblots at only one or a few time points. Comparison between different reports has been complicated by lack of specific antibodies and study-to-study variation in the HCMV strain used, with laboratory-adapted strains AD169 and Towne (which lack a large number of ORFs as a result of extensive passage in vitro) often used in preference to isolates containing a complete genome (Ma et al., 2012). We were able to quantify the temporal expression of the majority (139/171) of the canonical HCMV proteins as well as 14 noncanonical ORFs; most of these proteins were quantified in a single experiment (Figure 5, plots for all quantified viral proteins in Data S1).
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: firstname.lastname@example.org.