Quantitative temporal viromics: an approach to investigate host-pathogen interaction.
Bottom Line: QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets.Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined.QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: email@example.com.Show MeSH
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Mentions: The activity of a signaling pathway can be modulated either by posttranslational modification, or regulation of expression of a pathway member. Changes in protein expression can be quantified by QTV. We have shown that after an initial activation, the expression of ISG is rapidly reduced during HCMV infection, but how is this achieved? We quantified 13/15 key members of IFN induction and signaling pathways (Figure 3A, reviewed in Amsler et al., 2013). We confirmed known effects of HCMV infection on Jak1, STAT2, and IRF9 (Le et al., 2008) and demonstrated that, apart from STAT1, expression of the final effectors in both interferon induction and response pathways were all progressively diminished during infection (Figure 3A). An effect of HCMV infection on IRF3 has not previously been reported.
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: firstname.lastname@example.org.