Quantitative temporal viromics: an approach to investigate host-pathogen interaction.
Bottom Line: QTV predicted natural killer and T cell ligands, as well as 29 viral proteins present at the cell surface, potential therapeutic targets.Temporal profiles of >80% of HCMV canonical genes and 14 noncanonical HCMV open reading frames were defined.QTV is a powerful method that can yield important insights into viral infection and is applicable to any virus with a robust in vitro model.
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: firstname.lastname@example.org.Show MeSH
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Mentions: In general, we observed a striking correlation between the PM2 and WCL2 temporal profiles of all 29 high-confidence proteins. For the subset of known virion envelope glycoproteins (Varnum et al., 2004), protein expression at the PM was significantly later than in WCL, confirmed by analysis of the same proteins from experiments PM1 and WCL1 (Figures 6B and S6). This may reflect assembly of the HCMV virion within the viral cytoplasmic assembly compartment prior to viral egress (Alwine, 2012) and demonstrates that QTV can additionally provide insights into aspects of the viral life cycle. PM appearance of UL119 was similarly delayed, suggesting that this known virion glycoprotein may be a component of the virion envelope. The sensitivity of MS3 TMT-based mass spectrometry is suggested by our apparent quantitation of binding or fusion of viral envelope with the plasma membrane; there was a small early peak in PM presence of most virion envelope glycoproteins at 6 hr of infection that disappeared by 12 hr (Figure 6B).
Affiliation: Department of Cell Biology, Harvard Medical School, 240 Longwood Avenue, Boston, MA 02115, USA; Cambridge Institute for Medical Research, University of Cambridge, Hills Road, Cambridge CB2 0XY, UK. Electronic address: email@example.com.