Limits...
The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

Show MeSH

Related in: MedlinePlus

Ovarian morphology of rats from three groups (×100).A: PF. A lot of preantral follicles (P) and atretic follicles (AF) with enlargement of stroma (arrow) and cystic follicles (C) are shown in the section. B: PO. A lot of preantral follicles (P), atretic follicles (AF) and cystic follicles(C) are shown in the section. C: CO. Various stages of development follicles and some corpora lutea (CL) are shown. D: PF-SF. A lot of preantral follicles (P) and atretic follicles (AF), and cystic follicles(C) are shown in the section. E: PF-EF. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section. F: PF-EO. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section.
© Copyright Policy
Related In: Results  -  Collection

License
getmorefigures.php?uid=PMC4048306&req=5

pone-0099155-g003: Ovarian morphology of rats from three groups (×100).A: PF. A lot of preantral follicles (P) and atretic follicles (AF) with enlargement of stroma (arrow) and cystic follicles (C) are shown in the section. B: PO. A lot of preantral follicles (P), atretic follicles (AF) and cystic follicles(C) are shown in the section. C: CO. Various stages of development follicles and some corpora lutea (CL) are shown. D: PF-SF. A lot of preantral follicles (P) and atretic follicles (AF), and cystic follicles(C) are shown in the section. E: PF-EF. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section. F: PF-EO. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section.

Mentions: Ovarian morphological parameters were measured on the 28th day of experiment in three groups of CO, PO and PF (Fig. 3). Ovary organ index and ovarian areas were significant lower in PF than in CO. Corpora lutea in PF and PO were not observed. In comparison with CO group, thickness of theca layer, numbers of preantral follicle, numbers of atretic follicle, total numbers of follicle and ratios of numbers of atretic follicle to total numbers of follicles significantly increased in PF and PO, while thickness of granular layer significantly decreased in PF and PO (Table 2).


The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

Ovarian morphology of rats from three groups (×100).A: PF. A lot of preantral follicles (P) and atretic follicles (AF) with enlargement of stroma (arrow) and cystic follicles (C) are shown in the section. B: PO. A lot of preantral follicles (P), atretic follicles (AF) and cystic follicles(C) are shown in the section. C: CO. Various stages of development follicles and some corpora lutea (CL) are shown. D: PF-SF. A lot of preantral follicles (P) and atretic follicles (AF), and cystic follicles(C) are shown in the section. E: PF-EF. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section. F: PF-EO. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section.
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048306&req=5

pone-0099155-g003: Ovarian morphology of rats from three groups (×100).A: PF. A lot of preantral follicles (P) and atretic follicles (AF) with enlargement of stroma (arrow) and cystic follicles (C) are shown in the section. B: PO. A lot of preantral follicles (P), atretic follicles (AF) and cystic follicles(C) are shown in the section. C: CO. Various stages of development follicles and some corpora lutea (CL) are shown. D: PF-SF. A lot of preantral follicles (P) and atretic follicles (AF), and cystic follicles(C) are shown in the section. E: PF-EF. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section. F: PF-EO. A lot of antral follicles (A) and a bit of corpora lutea (CL) are shown in the section.
Mentions: Ovarian morphological parameters were measured on the 28th day of experiment in three groups of CO, PO and PF (Fig. 3). Ovary organ index and ovarian areas were significant lower in PF than in CO. Corpora lutea in PF and PO were not observed. In comparison with CO group, thickness of theca layer, numbers of preantral follicle, numbers of atretic follicle, total numbers of follicle and ratios of numbers of atretic follicle to total numbers of follicles significantly increased in PF and PO, while thickness of granular layer significantly decreased in PF and PO (Table 2).

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

Show MeSH
Related in: MedlinePlus