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The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

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Figures and cell types of vaginal smears.A: preoestrus, loaded mainly with epithelial cell; B: estrus, loaded mainly with keratinocytes; C:metoestrus,displaying epithelial cell, keratinocytes, and leukocytes; D:diestrus, displaying full of leukocytes (magnification ×100).
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pone-0099155-g002: Figures and cell types of vaginal smears.A: preoestrus, loaded mainly with epithelial cell; B: estrus, loaded mainly with keratinocytes; C:metoestrus,displaying epithelial cell, keratinocytes, and leukocytes; D:diestrus, displaying full of leukocytes (magnification ×100).

Mentions: The vaginal smears were monitored during whole experiment. The vaginas in all rats mostly opened at 40 days old. The regular estrous cycle of 4–5 days was observed in control (Fig. 2). However, there were many white cells, less epithelial cell, and keratinocytein vaginal smears of PO and PF rats, indicating these rats persisted in diestrus and no ovulation happened. After 2 weeks, the rats in PF-EF and PF-EO restored to normal estrous cycles, while the rats in PF-SF still showed persistent diestrus.


The characterization of obese polycystic ovary syndrome rat model suitable for exercise intervention.

Wu C, Lin F, Qiu S, Jiang Z - PLoS ONE (2014)

Figures and cell types of vaginal smears.A: preoestrus, loaded mainly with epithelial cell; B: estrus, loaded mainly with keratinocytes; C:metoestrus,displaying epithelial cell, keratinocytes, and leukocytes; D:diestrus, displaying full of leukocytes (magnification ×100).
© Copyright Policy
Related In: Results  -  Collection

License
Show All Figures
getmorefigures.php?uid=PMC4048306&req=5

pone-0099155-g002: Figures and cell types of vaginal smears.A: preoestrus, loaded mainly with epithelial cell; B: estrus, loaded mainly with keratinocytes; C:metoestrus,displaying epithelial cell, keratinocytes, and leukocytes; D:diestrus, displaying full of leukocytes (magnification ×100).
Mentions: The vaginal smears were monitored during whole experiment. The vaginas in all rats mostly opened at 40 days old. The regular estrous cycle of 4–5 days was observed in control (Fig. 2). However, there were many white cells, less epithelial cell, and keratinocytein vaginal smears of PO and PF rats, indicating these rats persisted in diestrus and no ovulation happened. After 2 weeks, the rats in PF-EF and PF-EO restored to normal estrous cycles, while the rats in PF-SF still showed persistent diestrus.

Bottom Line: Body weight, Lee index, FINS increased significantly in PF rat model.Serum levels of E2 and T were significantly higher in PF and PO than in CO.Ovary organ index and ovarian areas were significant lower in PF than in CO.

View Article: PubMed Central - PubMed

Affiliation: Department of Rehabilitation Medicine, The First Affiliated Hospital of Nanjing Medical University, Nanjing, China.

ABSTRACT

Objective: To develop a new polycystic ovary syndrome (PCOS) rat model suitable for exercise intervention.

Method: Thirty six rats were randomly divided into three experimental groups: PCOS rats with high-fat diet (PF, n = 24), PCOS rats with ordinary diet (PO, n = 6), and control rats with ordinary diet (CO, n = 6). Two kinds of PCOS rat model were made by adjustment diet structure and testosterone injection for 28 days. After a successful animal model, PF model rats were randomly assigned to three groups: exercise with a continuation of high-fat diet (PF-EF, n = 6), sedentary with a continuation of high-fat diet (PF-SF, n = 6), exercise with an ordinary diet (PF-EO, n = 6). Fasting blood glucose (FBG) and insulin (FINS), estrogen (E2), progesterone (P), and testosterone (T) in serum were determined by RIA, and ovarian morphology was evaluated by Image-Pro plus 6.0.

Results: Body weight, Lee index, FINS increased significantly in PF rat model. Serum levels of E2 and T were significantly higher in PF and PO than in CO. Ovary organ index and ovarian areas were significant lower in PF than in CO. After intervention for 2 weeks, the levels of 1 h postprandial blood glucose (PBG1), 2 h postprandial blood glucose (PBG2), FINS and the serum levels of T decreased significantly in PF-EF rats and PF-EO rats. The ratio of FBG/FINS was significant higher in PF-EO rats than in PF-SF rats. Ovarian morphology showed that the numbers of preantral follicles and atretic follicles decreased significantly, and the numbers of antral follicles and corpora lutea increased significantly in the rats of PF-EF and PF-EO.

Conclusion: By combination of high-fat diet and testosterone injection, the obese PCOS rat model is conformable with the lifestyle habits of fatty foods and insufficient exercise, and has metabolic and reproductive characteristics of human PCOS. This model can be applied to study exercise intervention.

Show MeSH
Related in: MedlinePlus